Alignments for a candidate for metA in Shewanella oneidensis MR-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for metA in Shewanella oneidensis MR-1

Align Homoserine O-succinyltransferase; HST; Homoserine transsuccinylase; HTS; EC 2.3.1.46 (characterized)
to candidate 200841 SO1676 homoserine O-succinyltransferase (NCBI ptt file)

Query= SwissProt::A6WLE9
         (313 letters)



>FitnessBrowser__MR1:200841
          Length = 313

 Score =  625 bits (1612), Expect = 0.0
 Identities = 299/313 (95%), Positives = 307/313 (98%)

Query: 1   MPVRIPDHLPAAEVLESENIFVMSETRAANQDIRPMKVLILNLMPNKIETETQLLRLLGN 60
           MPV+IPDHLPAA +LESENIFVMSETRAANQDIRPMKVLILNLMPNKIETETQLLRLLGN
Sbjct: 1   MPVKIPDHLPAAGILESENIFVMSETRAANQDIRPMKVLILNLMPNKIETETQLLRLLGN 60

Query: 61  TPLQVDVDLLRIHDKESKHTSIDHMNTFYRDFEAVRHKNYDGLIITGAPLGQIDFEDVVY 120
           TPLQVDVDLLRIHDKESKHTSIDHMNTFYRDFE VRHKNYDGLIITGAPLGQIDFEDV Y
Sbjct: 61  TPLQVDVDLLRIHDKESKHTSIDHMNTFYRDFEDVRHKNYDGLIITGAPLGQIDFEDVTY 120

Query: 121 WDHIREIIDWSQEHVTSVLFLCWAAHAGLYHLYGLNRKILQQKRSGVFVHRRTSQHFPLL 180
           WDHIREIIDWSQ+HVTSVLFLCWAAHAGLYHLYGLNRKIL+QKRSGVFVHRRT QHFPLL
Sbjct: 121 WDHIREIIDWSQQHVTSVLFLCWAAHAGLYHLYGLNRKILEQKRSGVFVHRRTCQHFPLL 180

Query: 181 RGFDDEFFAPHSRFAEMDVEEIRQHPQLQLLAESDEAGAYLVLSRNNRNLFVMGHPEYQK 240
           RGFDDEFFAPHSRFAEMDVEEIRQHPQLQLLAESDEAGAYLVLSRNNRNLFVMGHPEYQK
Sbjct: 181 RGFDDEFFAPHSRFAEMDVEEIRQHPQLQLLAESDEAGAYLVLSRNNRNLFVMGHPEYQK 240

Query: 241 STLNEEYQRDLSQGLDPNVPQNYYRNDDPKADAIARWHSHGSLLVSNWLNYYVYQLTPYD 300
           STLN+EY RDL+Q L+PNVPQNYYRNDDPKADAIARWHSHGSLLVSNWLNYYVYQLTPYD
Sbjct: 241 STLNDEYHRDLAQSLNPNVPQNYYRNDDPKADAIARWHSHGSLLVSNWLNYYVYQLTPYD 300

Query: 301 LSDMTAMTPWESR 313
           LSDMTAMTPWES+
Sbjct: 301 LSDMTAMTPWESQ 313


Lambda     K      H
   0.321    0.136    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 507
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 313
Length of database: 313
Length adjustment: 27
Effective length of query: 286
Effective length of database: 286
Effective search space:    81796
Effective search space used:    81796
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate 200841 SO1676 (homoserine O-succinyltransferase (NCBI ptt file))
to HMM TIGR01001 (metA: homoserine O-succinyltransferase (EC 2.3.1.46))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01001.hmm
# target sequence database:        /tmp/gapView.7136.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01001  [M=301]
Accession:   TIGR01001
Description: metA: homoserine O-succinyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                       -----------
   1.7e-144  466.9   0.5   1.9e-144  466.8   0.5    1.0  1  lcl|FitnessBrowser__MR1:200841  SO1676 homoserine O-succinyltran


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__MR1:200841  SO1676 homoserine O-succinyltransferase (NCBI ptt file)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  466.8   0.5  1.9e-144  1.9e-144       1     300 [.       1     301 [.       1     302 [. 0.99

  Alignments for each domain:
  == domain 1  score: 466.8 bits;  conditional E-value: 1.9e-144
                       TIGR01001   1 mpirvpdelpavellkeenifvmtekrashqdirplevlilnlmpkkietenqllrllsnsplqvditllridsrkskn 79 
                                     mp+++pd+lpa  +l+ enifvm+e ra +qdirp++vlilnlmp+kiete+qllrll+n+plqvd++llri++++sk+
  lcl|FitnessBrowser__MR1:200841   1 MPVKIPDHLPAAGILESENIFVMSETRAANQDIRPMKVLILNLMPNKIETETQLLRLLGNTPLQVDVDLLRIHDKESKH 79 
                                     9****************************************************************************** PP

                       TIGR01001  80 tpiehlekfykeleevkdrkfdGlivtGapvellefedvayweelkeilewskenvtstlyicwaaqaalkllygipkr 158
                                     t i+h+++fy+++e+v+++++dGli+tGap+++++fedv yw++++ei++ws+++vts l++cwaa+a+l+ lyg++++
  lcl|FitnessBrowser__MR1:200841  80 TSIDHMNTFYRDFEDVRHKNYDGLIITGAPLGQIDFEDVTYWDHIREIIDWSQQHVTSVLFLCWAAHAGLYHLYGLNRK 158
                                     ******************************************************************************* PP

                       TIGR01001 159 tleeklsGvykhdiv.kedlllrgfddkflaphsryadldeeliaeltdleilaesdeagvylaaskdernifvtGhpe 236
                                      le+k sGv+ h+ + ++ +llrgfdd+f+aphsr+a++d e+i+++++l++laesdeag+yl++s+++rn+fv+Ghpe
  lcl|FitnessBrowser__MR1:200841 159 ILEQKRSGVFVHRRTcQHFPLLRGFDDEFFAPHSRFAEMDVEEIRQHPQLQLLAESDEAGAYLVLSRNNRNLFVMGHPE 237
                                     *************9978999*********************************************************** PP

                       TIGR01001 237 ydketlrqeyvrdvgeglkvdipknyypkddpektpiaswrshanllfanwlnyavyqktpydl 300
                                     y+k tl++ey rd+ ++l++++p+nyy +ddp+   ia w+sh+ ll +nwlny+vyq tpydl
  lcl|FitnessBrowser__MR1:200841 238 YQKSTLNDEYHRDLAQSLNPNVPQNYYRNDDPKADAIARWHSHGSLLVSNWLNYYVYQLTPYDL 301
                                     **************************************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (301 nodes)
Target sequences:                          1  (313 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.24
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis