Align Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 (characterized)
to candidate 203140 SO4056 cystathionine gamma-synthase (NCBI ptt file)
Query= SwissProt::P00935 (386 letters) >FitnessBrowser__MR1:203140 Length = 393 Score = 462 bits (1188), Expect = e-135 Identities = 235/380 (61%), Positives = 286/380 (75%), Gaps = 1/380 (0%) Query: 3 RKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAHDYSRRGNPTRDVVQRALA 62 R+ AT+AVR G+ D QYG VVPPI+LS+ Y F G PR DYSR GNPTR ++ ALA Sbjct: 10 RQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRSILGDALA 69 Query: 63 ELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYGGSYRLFDSLAKRGCYRVLFV 122 +LE GA V+T TGM+AI LVTT+ L P DLLV PHDCYGGSYRLF +LAK+G +++L V Sbjct: 70 KLEKGATGVVTCTGMAAITLVTTL-LGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVV 128 Query: 123 DQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKICHLAREVGAVSVVDNTFLSPALQ 182 DQ D QAL A+A++PK+V +E+PSNPLLRVVDI I + VGA+ VVDNTFLSP LQ Sbjct: 129 DQTDNQALAQAIAQQPKMVWIETPSNPLLRVVDIEAIAKASHGVGALVVVDNTFLSPILQ 188 Query: 183 NPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDVVTELAWWANNIGVTGGAFDSYLLL 242 PL LGAD+V+HS TKY+NGHSDVV G VIAKDP + L WW+N +G+TG AFDSY L Sbjct: 189 QPLLLGADIVIHSTTKYINGHSDVVGGAVIAKDPQIGETLHWWSNTLGLTGSAFDSYQTL 248 Query: 243 RGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLYHPSLPENQGHEIAARQQKGFGAMLSF 302 RGLRTL R+ QRNAQ IV+ L P+V K+Y+P L ++ GH IAA+QQKGFGAMLSF Sbjct: 249 RGLRTLAVRIREHQRNAQRIVELLSNSPVVSKVYYPGLADHPGHAIAAKQQKGFGAMLSF 308 Query: 303 ELDGDEQTLRRFLGGLSLFTLAESLGGVESLISHAATMTHAGMAPEARAAAGISETLLRI 362 EL G E + FL LSLF++AESLGGVESL++ ATMTH M P+AR AGI +TLLR+ Sbjct: 309 ELKGGEAEVVAFLDALSLFSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLLRL 368 Query: 363 STGIEDGEDLIADLENGFRA 382 S GIED +DL+AD++ G A Sbjct: 369 SVGIEDADDLVADIQAGLAA 388 Lambda K H 0.319 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 456 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 386 Length of database: 393 Length adjustment: 30 Effective length of query: 356 Effective length of database: 363 Effective search space: 129228 Effective search space used: 129228 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate 203140 SO4056 (cystathionine gamma-synthase (NCBI ptt file))
to HMM TIGR02080 (metB: O-succinylhomoserine (thiol)-lyase (EC 2.5.1.48))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR02080.hmm # target sequence database: /tmp/gapView.24411.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02080 [M=382] Accession: TIGR02080 Description: O_succ_thio_ly: O-succinylhomoserine (thiol)-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-212 691.2 0.0 1.8e-212 690.9 0.0 1.0 1 lcl|FitnessBrowser__MR1:203140 SO4056 cystathionine gamma-synth Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:203140 SO4056 cystathionine gamma-synthase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 690.9 0.0 1.8e-212 1.8e-212 2 382 .] 10 389 .. 9 389 .. 0.99 Alignments for each domain: == domain 1 score: 690.9 bits; conditional E-value: 1.8e-212 TIGR02080 2 rkkatiavrsGlesdkqygavvpPiylsttyefagfnepraydysrsgnPtrdllekalaelekGadavvtssGmsaie 80 r+ at+avr+G+esd+qygavvpPiylst+y+f+g+++pr++dysrsgnPtr++l +ala+lekGa++vvt++Gm+ai+ lcl|FitnessBrowser__MR1:203140 10 RQLATLAVRQGIESDTQYGAVVPPIYLSTNYAFDGHKNPREFDYSRSGNPTRSILGDALAKLEKGATGVVTCTGMAAIT 88 6889*************************************************************************** PP TIGR02080 81 llviallkpddllvaPhdcyGGtyrllkalakkgklkvqlvdqsdeealekalaekpklvlietPsnPllrvvdiaklc 159 l++ +ll+pddllv+PhdcyGG+yrl+++lakkg++k+++vdq+d++al++a+a++pk+v+ietPsnPllrvvdi++++ lcl|FitnessBrowser__MR1:203140 89 LVT-TLLGPDDLLVVPHDCYGGSYRLFTNLAKKGQFKLLVVDQTDNQALAQAIAQQPKMVWIETPSNPLLRVVDIEAIA 166 *99.9************************************************************************** PP TIGR02080 160 klakaagavvvvdntflsPilqkPlalGadlvlhsatkylnGhsdviaGaviakdkqlaeelawwanalGvtgaafdsy 238 k+++ +ga+vvvdntflsPilq+Pl lGad+v+hs+tky+nGhsdv++Gaviakd+q+ e+l+ww+n+lG+tg+afdsy lcl|FitnessBrowser__MR1:203140 167 KASHGVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVIAKDPQIGETLHWWSNTLGLTGSAFDSY 245 ******************************************************************************* PP TIGR02080 239 lllrGlrtlaarvreqernakkiveylqkqplvkkvyypglpdhagheiaakqqkGfGallsfelkGgeeevkkflkkl 317 ++lrGlrtla+r+re++rna++ive+l+++p+v+kvyypgl+dh+gh+iaakqqkGfGa+lsfelkGge+ev +fl++l lcl|FitnessBrowser__MR1:203140 246 QTLRGLRTLAVRIREHQRNAQRIVELLSNSPVVSKVYYPGLADHPGHAIAAKQQKGFGAMLSFELKGGEAEVVAFLDAL 324 ******************************************************************************* PP TIGR02080 318 klftlaeslGGvesliahpatmthaamekeareeaGikdellrlsvGledaddliadleqalaav 382 +lf++aeslGGvesl+a+patmth+ame++ar+eaGikd+llrlsvG+edaddl+ad++++laav lcl|FitnessBrowser__MR1:203140 325 SLFSVAESLGGVESLVAVPATMTHRAMEPQARFEAGIKDTLLRLSVGIEDADDLVADIQAGLAAV 389 **************************************************************985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (382 nodes) Target sequences: 1 (393 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.44 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory