Align homoserine kinase; EC 2.7.1.39 (characterized)
to candidate 202521 SO3414 homoserine kinase (NCBI ptt file)
Query= CharProtDB::CH_002113 (310 letters) >FitnessBrowser__MR1:202521 Length = 312 Score = 342 bits (877), Expect = 7e-99 Identities = 170/305 (55%), Positives = 219/305 (71%), Gaps = 2/305 (0%) Query: 4 VYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAET-FSLNNLGRFADKLPSEPRE 62 VYAPAS N+ VGFD+LGAA+ P+DG+LLGD VT+ AA+ SL +G +A KLPS P + Sbjct: 5 VYAPASMGNVGVGFDLLGAALAPIDGSLLGDRVTIAAADGGVSLTQVGGWAHKLPSNPED 64 Query: 63 NIVYQCWERFCQELGKQIP-VAMTLEKNMPIGSGLGSSACSVVAALMAMNEHCGKPLNDT 121 NIV+QC F + LGK+ VA+TLEKN+P+GSGLGSSA SVVAAL A+NEH G P + Sbjct: 65 NIVHQCAVFFLKALGKENRGVALTLEKNLPVGSGLGSSASSVVAALYALNEHFGCPYDKQ 124 Query: 122 RLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDIISQQVPGFDEWLWVLAYPGIK 181 LL LMGE EG+ISGS+HYDNVAPC+LGGMQLM+ I + +P F +W WV+AYPGI Sbjct: 125 ALLELMGEFEGKISGSVHYDNVAPCYLGGMQLMLNVPGAICEAIPSFKQWYWVVAYPGIS 184 Query: 182 VSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQPELAAKLMKDVIAEPYRERLLPG 241 +STA R ++P QY + I GR+L+ F+HA Y + +LA +++KDV+AEPYR +PG Sbjct: 185 LSTAMMRKLMPEQYDKSVVIDFGRYLSAFVHASYQQNAQLAIQVLKDVLAEPYRAAAIPG 244 Query: 242 FRQARQAVAEIGAVASGISGSGPTLFALCDKPETAQRVADWLGKNYLQNQEGFVHICRLD 301 + AR A+AE+G + +GISGSGPTLF++ D TA WL NYL + GF H+CRLD Sbjct: 245 YDNARAALAELGMLTTGISGSGPTLFSVTDDLATANAAKAWLEANYLTDAGGFAHVCRLD 304 Query: 302 TAGAR 306 G R Sbjct: 305 EQGTR 309 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 291 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 310 Length of database: 312 Length adjustment: 27 Effective length of query: 283 Effective length of database: 285 Effective search space: 80655 Effective search space used: 80655 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
Align candidate 202521 SO3414 (homoserine kinase (NCBI ptt file))
to HMM TIGR00191 (thrB: homoserine kinase (EC 2.7.1.39))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00191.hmm # target sequence database: /tmp/gapView.14387.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00191 [M=304] Accession: TIGR00191 Description: thrB: homoserine kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.4e-91 291.7 0.0 2.7e-91 291.5 0.0 1.0 1 lcl|FitnessBrowser__MR1:202521 SO3414 homoserine kinase (NCBI p Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:202521 SO3414 homoserine kinase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 291.5 0.0 2.7e-91 2.7e-91 1 300 [. 3 309 .. 3 312 .] 0.98 Alignments for each domain: == domain 1 score: 291.5 bits; conditional E-value: 2.7e-91 TIGR00191 1 lkvkvPassANlgpGfDvlGlalslvlellvtedvaqeskdk..sleaegegvekipkesdkNliyqvakkvlkklgkr 77 l+v++Pas N+g+GfD lG+al +++++l+++ v+++++d sl+++g ++k+p+++++N++ q+a+ +lk lgk+ lcl|FitnessBrowser__MR1:202521 3 LTVYAPASMGNVGVGFDLLGAALAPIDGSLLGDRVTIAAADGgvSLTQVGGWAHKLPSNPEDNIVHQCAVFFLKALGKE 81 68*********************************999999899*********************************** PP TIGR00191 78 vkpvkltvekeiplgrGLGSSaaaivaaviaanelaglklskeelldlalllEg......HpDNvapallGGlqlavke 150 +v+lt+ek++p+g+GLGSSa+++vaa++a+ne++g +++k+ ll+l+++ Eg H+DNvap++lGG+ql+++ lcl|FitnessBrowser__MR1:202521 82 NRGVALTLEKNLPVGSGLGSSASSVVAALYALNEHFGCPYDKQALLELMGEFEGkisgsvHYDNVAPCYLGGMQLMLNV 160 ******************************************************************************* PP TIGR00191 151 ddllevlkvPsgsklkvvlviPnievsTaeaRavLPkaysrqdlvfnlshlavlvtAlvskdkadllaiamkDrvhqpy 229 +++ +Ps++++++v+++P+i++sTa R+++P++y+ + ++ ++ l+ +v+A +++ +a+l+ ++kD++++py lcl|FitnessBrowser__MR1:202521 161 PG-AICEAIPSFKQWYWVVAYPGISLSTAMMRKLMPEQYDKSVVIDFGRYLSAFVHASYQQ-NAQLAIQVLKDVLAEPY 237 99.**********************************************************.***************** PP TIGR00191 230 RekliPelteikqaakekgalgitlSGaGptilalaeeek.eekaqelleklakegieltvkvleldtdgae 300 R+ iP++++ + a +e g+l++++SG+Gpt+++++++ ++ a+ +le ++ ++ +v++ld +g++ lcl|FitnessBrowser__MR1:202521 238 RAAAIPGYDNARAALAELGMLTTGISGSGPTLFSVTDDLAtANAAKAWLEANYLTDAGGFAHVCRLDEQGTR 309 ***************************************99999*************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (304 nodes) Target sequences: 1 (312 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 11.20 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory