Align threonine synthase (EC 4.2.3.1) (characterized)
to candidate 202520 SO3413 threonine synthase (NCBI ptt file)
Query= BRENDA::P00934 (428 letters) >FitnessBrowser__MR1:202520 Length = 427 Score = 478 bits (1230), Expect = e-139 Identities = 248/421 (58%), Positives = 304/421 (72%), Gaps = 2/421 (0%) Query: 1 MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEIDEMLKLDFVTRSAKILS 60 M+LYNLK ++ VSF +AV GLGK++GLFFP +P L +I+ +L + FV RS K+L Sbjct: 1 MELYNLKHPSQTVSFKEAVQLGLGKDRGLFFPTQIPV--LHDIEALLAMPFVERSKKVLG 58 Query: 61 AFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGPTLAFKDFGGRFMAQMLTH 120 A++ E+ Q+++++ V AF F P+ V+ CLELFHGPTLAFKDFG RFMAQ + Sbjct: 59 AWLASELGQDMVDQLVERAFNFDLPLVAVDEQRACLELFHGPTLAFKDFGARFMAQCINV 118 Query: 121 IAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRGKISPLQEKLFCTLGGNIETV 180 A D +TILTATSGDTGAAVA AFYGL V VV+LYP+GKIS LQEK+F TLG NI TV Sbjct: 119 FAQDSRLTILTATSGDTGAAVADAFYGLEKVNVVVLYPKGKISELQEKMFTTLGKNIHTV 178 Query: 181 AIDGDFDACQALVKQAFDDEELKVALGLNSANSINISRLLAQICYYFEAVAQLPQETRNQ 240 A+ DFDACQ LVKQAF+D +++ L LNSANSINISRLLAQICYYFEAVAQ Q Sbjct: 179 AVASDFDACQHLVKQAFEDSDVRDGLHLNSANSINISRLLAQICYYFEAVAQSKQRHEAD 238 Query: 241 LVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVNDTVPRFLHDGQWSPKATQATLSNA 300 V++VPSGNFG+LTAGL AK++GLPVKRFIAATN NDTVPR+L G W P TQAT+SNA Sbjct: 239 PVIAVPSGNFGNLTAGLFAKAMGLPVKRFIAATNANDTVPRYLDSGHWQPNPTQATMSNA 298 Query: 301 MDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDETTQQTMRELKELGYTSEPHAAVAYRA 360 MDVS+P+NWPRVE + + W L +L + + T + + +L GY SEPHAA+A +A Sbjct: 299 MDVSEPSNWPRVEAICEKLGWPLADLVGICLSEAQTSEALVDLYAKGYVSEPHAAIAAKA 358 Query: 361 LRDQLNPGEYGLFLGTAHPAKFKESVEAILGETLDLPKELAERADLPLLSHNLPADFAAL 420 L L P E G+FLGTAHPAKFK+ V+ L L LP EL A P+LS L ADFA L Sbjct: 359 LTLNLVPDEVGIFLGTAHPAKFKDVVDRELALNLPLPPELKAVAHKPILSAELAADFAQL 418 Query: 421 R 421 + Sbjct: 419 K 419 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 509 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 428 Length of database: 427 Length adjustment: 32 Effective length of query: 396 Effective length of database: 395 Effective search space: 156420 Effective search space used: 156420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate 202520 SO3413 (threonine synthase (NCBI ptt file))
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.28079.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.3e-101 323.5 0.0 1e-100 323.2 0.0 1.0 1 lcl|FitnessBrowser__MR1:202520 SO3413 threonine synthase (NCBI Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:202520 SO3413 threonine synthase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 323.2 0.0 1e-100 1e-100 12 329 .. 64 385 .. 50 392 .. 0.90 Alignments for each domain: == domain 1 score: 323.2 bits; conditional E-value: 1e-100 TIGR00260 12 ekdlvdlaegstelfrspklaeevgaenlyvkelfhgPtlaFKDrglqfvavlltkalelgnetvlcAtsGdtgaaaae 90 e + +++ + ++f+++ + v + + ++elfhgPtlaFKD+g +f+a+ ++ ++++ + t+l+AtsGdtgaa+a lcl|FitnessBrowser__MR1:202520 64 ELGQDMVDQLVERAFNFDLPLVAVDE-QRACLELFHGPTLAFKDFGARFMAQCINVFAQDSRLTILTATSGDTGAAVAD 141 45555666777788888888888887.6689************************************************ PP TIGR00260 91 alagkanvkvvvLyPkgkispvkeklvtalaenakvlaikGdFDdaqdlvkeifedke...klklnsvNsinparieaq 166 a++g + v+vvvLyPkgkis ++ek++t+l +n++++a+ dFD++q+lvk++fed+ l lns+Nsin++r++aq lcl|FitnessBrowser__MR1:202520 142 AFYGLEKVNVVVLYPKGKISELQEKMFTTLGKNIHTVAVASDFDACQHLVKQAFEDSDvrdGLHLNSANSINISRLLAQ 220 *******************************************************7777789***************** PP TIGR00260 167 ktyafeiveqlgkespdkvvvpvpsgnfgailkGflekkelglpieklaiaaegaadivrrflksgdlepkedkeTlst 245 ++y+fe+v+q +++++ v++vpsgnfg++++G ++k ++ lp k++iaa++a+d+v+r+l sg+++p +++T+s+ lcl|FitnessBrowser__MR1:202520 221 ICYYFEAVAQSKQRHEADPVIAVPSGNFGNLTAGLFAKAMG-LP-VKRFIAATNANDTVPRYLDSGHWQPNPTQATMSN 297 **********************************9999998.**.99******************************** PP TIGR00260 246 Amdignpsnveralelarrslgnledlkesv.sdeeileaikklaeeegyllephtavavaalkklvekg.vs...ata 319 Amd+++psn++r++++ ++ ++ l dl + s+++++ea l++ +gy+ eph+a+a +al + + v+ +ta lcl|FitnessBrowser__MR1:202520 298 AMDVSEPSNWPRVEAICEKLGWPLADLVGIClSEAQTSEALVDLYA-KGYVSEPHAAIAAKALTLNLVPDeVGiflGTA 375 **********************999986655155666667666665.8**************97666666555778*** PP TIGR00260 320 dpaKFeevve 329 +paKF++vv lcl|FitnessBrowser__MR1:202520 376 HPAKFKDVVD 385 ********96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (427 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01 # Mc/sec: 14.17 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory