Alignments for a candidate for aspS2 in Marinobacter adhaerens HP15

GapMind for Amino acid biosynthesis

Alignments for a candidate for aspS2 in Marinobacter adhaerens HP15

Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate GFF2090 HP15_2044 aspartyl-tRNA synthetase

Query= SwissProt::Q51422
         (591 letters)



>FitnessBrowser__Marino:GFF2090
          Length = 602

 Score =  894 bits (2311), Expect = 0.0
 Identities = 433/588 (73%), Positives = 499/588 (84%)

Query: 2   MRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAKA 61
           MRSHYCG +NES   QEVTLCGWVHRRRDHGGVIFLD+RDR+G++QVV DPD  E+FA A
Sbjct: 1   MRSHYCGGINESHIDQEVTLCGWVHRRRDHGGVIFLDLRDRDGMSQVVVDPDTPESFALA 60

Query: 62  DRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVGE 121
           ++VRSEFV+K+TG+VR RP G  N NM +G +E+LG EL +LN A TPPFPLDE+ DVGE
Sbjct: 61  EKVRSEFVIKVTGRVRRRPAGTENNNMPTGQVELLGKELTILNAAATPPFPLDEHVDVGE 120

Query: 122 ETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYLV 181
           + RLRYRF+DLRRPEM  +L+ R+R+TS IR YLD NGF+DVETPIL R TPEGARDYLV
Sbjct: 121 DVRLRYRFVDLRRPEMINRLRFRSRVTSYIRNYLDSNGFMDVETPILTRATPEGARDYLV 180

Query: 182 PSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETSF 241
           PSRT+ G FFALPQSPQLFKQLLMV+G DRYYQIAKCFRDEDLRADRQPEFTQ+DIE SF
Sbjct: 181 PSRTHEGSFFALPQSPQLFKQLLMVSGVDRYYQIAKCFRDEDLRADRQPEFTQVDIEASF 240

Query: 242 LDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVADQ 301
           +DE  ++ + E M+R LFK+VL+VE   FP MP+ EAM+RYGSDKPDLRIPLEL+DV D 
Sbjct: 241 IDEETLMKLNEDMIRALFKDVLEVELPSFPQMPYAEAMQRYGSDKPDLRIPLELLDVNDL 300

Query: 302 LKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNERA 361
           ++ V+FKVF+GPA DPKGRVAALRVP    + R QIDDYTKFVGIYGAKGLAYIKVN+ +
Sbjct: 301 VEGVDFKVFAGPAKDPKGRVAALRVPKGGELTRKQIDDYTKFVGIYGAKGLAYIKVNDLS 360

Query: 362 KGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDLK 421
           KGVEGLQSPI+KF+ +     I++RVGA DGDIVFFGADK  +V +ALGALRIK+GHDL 
Sbjct: 361 KGVEGLQSPIIKFLGDDVALAIMERVGAEDGDIVFFGADKTNVVNEALGALRIKIGHDLD 420

Query: 422 LLTREWAPMWVVDFPMFEENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMVLNG 481
           +LT EWAP+WVVDFPMFEE  DG L+A+HHPFT+P C+P +L A+P  ALSRAYDMVLNG
Sbjct: 421 MLTSEWAPLWVVDFPMFEETPDGGLTAIHHPFTAPSCSPEDLAADPANALSRAYDMVLNG 480

Query: 482 TELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLVM 541
           TELGGGSIRIHD+ MQ+AVFR+LGI E E   KFGFLLDALK+G PPHGGLAFGLDRLVM
Sbjct: 481 TELGGGSIRIHDEKMQEAVFRILGIGEEEARAKFGFLLDALKFGCPPHGGLAFGLDRLVM 540

Query: 542 LMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPK 589
           LMTGASSIR+VIAFPKTQSA  +MTQAPG VD K LRELHIRLR+  K
Sbjct: 541 LMTGASSIRDVIAFPKTQSATCLMTQAPGEVDEKQLRELHIRLRKPSK 588


Lambda     K      H
   0.321    0.140    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1258
Number of extensions: 51
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 591
Length of database: 602
Length adjustment: 37
Effective length of query: 554
Effective length of database: 565
Effective search space:   313010
Effective search space used:   313010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis