GapMind for Amino acid biosynthesis

 

Alignments for a candidate for aroD in Marinobacter adhaerens HP15

Align 3-dehydroquinate dehydratase (EC 4.2.1.10) (characterized)
to candidate GFF3278 HP15_3220 dehydroquinase, class II

Query= BRENDA::O30557
         (147 letters)



>FitnessBrowser__Marino:GFF3278
          Length = 133

 Score =  203 bits (516), Expect = 9e-58
 Identities = 99/133 (74%), Positives = 111/133 (83%)

Query: 14  LLGTREPGTYGSTTLGQINQDLERRAREAGHHLLHLQSNAEYELIDRIHAARDEGVDFII 73
           +LGTREP  YG  TL  I+  L   A E GHHLLHLQSNAEYELI+R+H AR EGVDFII
Sbjct: 1   MLGTREPEVYGYETLADIDDRLRSLAAEQGHHLLHLQSNAEYELIERVHEARAEGVDFII 60

Query: 74  INPAAFTHTSVALRDALLAVSIPFIEVHLSNVHKREPFRHHSYFSDVAVGVICGLGATGY 133
           INPAAFTHTSVALRDA+LA  IPFIEVHLSNVH REPFRHHSYFSD+A GVICGLG+ GY
Sbjct: 61  INPAAFTHTSVALRDAMLASGIPFIEVHLSNVHAREPFRHHSYFSDIAEGVICGLGSQGY 120

Query: 134 RLALESALEQLQR 146
            LAL++AL+++ R
Sbjct: 121 DLALQAALKRIHR 133


Lambda     K      H
   0.322    0.138    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 122
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 147
Length of database: 133
Length adjustment: 15
Effective length of query: 132
Effective length of database: 118
Effective search space:    15576
Effective search space used:    15576
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 42 (20.8 bits)

Align candidate GFF3278 HP15_3220 (dehydroquinase, class II)
to HMM TIGR01088 (aroQ: 3-dehydroquinate dehydratase, type II (EC 4.2.1.10))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01088.hmm
# target sequence database:        /tmp/gapView.21878.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01088  [M=141]
Accession:   TIGR01088
Description: aroQ: 3-dehydroquinate dehydratase, type II
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                           Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                           -----------
    7.2e-57  177.1   0.1    8.1e-57  177.0   0.1    1.0  1  lcl|FitnessBrowser__Marino:GFF3278  HP15_3220 dehydroquinase, class 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Marino:GFF3278  HP15_3220 dehydroquinase, class II
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  177.0   0.1   8.1e-57   8.1e-57      12     139 ..       1     129 [.       1     131 [. 0.98

  Alignments for each domain:
  == domain 1  score: 177.0 bits;  conditional E-value: 8.1e-57
                           TIGR01088  12 lLGkrepkvyGsltleeieelleeaakelevevevfqsnsegelidkihealeq.vdgivinpaalthtsvalrD 85 
                                         +LG+rep+vyG  tl +i++ l++ a+e++ ++ ++qsn e+eli+++hea  + vd+i+inpaa+thtsvalrD
  lcl|FitnessBrowser__Marino:GFF3278   1 MLGTREPEVYGYETLADIDDRLRSLAAEQGHHLLHLQSNAEYELIERVHEARAEgVDFIIINPAAFTHTSVALRD 75 
                                         8**************************************************9888******************** PP

                           TIGR01088  86 alaavslPvvevhlsnvhareefrkksvlaevakGvivGlGakgyklalealve 139
                                         a++a  +P++evhlsnvhare+fr++s+++++a+Gvi+GlG +gy lal+a+++
  lcl|FitnessBrowser__Marino:GFF3278  76 AMLASGIPFIEVHLSNVHAREPFRHHSYFSDIAEGVICGLGSQGYDLALQAALK 129
                                         *************************************************99876 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (141 nodes)
Target sequences:                          1  (133 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 3.98
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory