Align diaminopimelate decarboxylase (EC 4.1.1.20) (characterized)
to candidate GFF248 HP15_247 diaminopimelate decarboxylase
Query= BRENDA::Q9KVL7 (417 letters) >FitnessBrowser__Marino:GFF248 Length = 416 Score = 600 bits (1546), Expect = e-176 Identities = 299/418 (71%), Positives = 353/418 (84%), Gaps = 3/418 (0%) Query: 1 MDYFNYQEDGQLWAEQVPLADLANQYGTPLYVYSRATLERHWHAFDKSVGDYPHLICYAV 60 MD+FNY+ DG+L+AE +P++ +A+++GTP YVYSRATLERH+ A+D ++ D+PHL+CYAV Sbjct: 1 MDHFNYR-DGELYAEDIPVSAIADRFGTPAYVYSRATLERHYRAYDDALRDHPHLVCYAV 59 Query: 61 KANSNLGVLNTLARLGSGFDIVSVGELERVLAAGGDPSKVVFSGVGKTEAEMKRALQLKI 120 KANSNL VLN LARLG+GFDIVS GELERVL AGGDPS+VVFSGVGK E EMKRAL++ + Sbjct: 60 KANSNLAVLNVLARLGAGFDIVSAGELERVLRAGGDPSRVVFSGVGKQEWEMKRALEVGV 119 Query: 121 KCFNVESEPELQRLNKVAGELGVKAPISLRINPDVDAKTHPYISTGLRDNKFGITFDRAA 180 +CFNVES+ EL RLN VAGELGVKAPISLR+NPDVDA THPYISTGL++NKFGI A+ Sbjct: 120 RCFNVESDTELDRLNAVAGELGVKAPISLRVNPDVDAGTHPYISTGLKENKFGIDIAEAS 179 Query: 181 QVYRLAHSLPNLDVHGIDCHIGSQLTALAPFIDATDRLLALIDSLKAEGIHIRHLDVGGG 240 QVY A +PNLDV G+DCHIGSQLT+++PF+DA DR+LALIDSL +GIHI HLD+GGG Sbjct: 180 QVYARAAGMPNLDVKGVDCHIGSQLTSVSPFLDALDRVLALIDSLADQGIHIHHLDMGGG 239 Query: 241 LGVVYRDELPPQPSEYAKALLDRLERHRDLELIFEPGRAIAANAGVLVTKVEFLKHTEHK 300 LGV Y E PPQPS+Y KAL +RL R LELI EPGR+IAANAG+LVT+VEFLK TEH+ Sbjct: 240 LGVTYNQEQPPQPSDYVKALAERL-GDRKLELILEPGRSIAANAGILVTRVEFLKCTEHR 298 Query: 301 NFAIIDAAMNDLIRPALYQAWQDIIPLRPRQ-GEAQTYDLVGPVCETSDFLGKDRDLVLQ 359 NFAIIDAAMNDLIRPALY AWQ IIP++P Q GE + +DLVGPVCET DFLGKDR L LQ Sbjct: 299 NFAIIDAAMNDLIRPALYSAWQAIIPVKPHQDGEEKAWDLVGPVCETGDFLGKDRHLRLQ 358 Query: 360 EGDLLAVRSSGAYGFTMSSNYNTRPRVAEVMVDGNKTYLVRQREELSSLWALESVLPE 417 GDLLAVRS+GAYGF MSSNYNTR R E+MVDG++ ++VR+RE L A ES LPE Sbjct: 359 AGDLLAVRSAGAYGFVMSSNYNTRNRPPELMVDGDQVHVVRRRETLEDQLAPESCLPE 416 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 572 Number of extensions: 19 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 417 Length of database: 416 Length adjustment: 31 Effective length of query: 386 Effective length of database: 385 Effective search space: 148610 Effective search space used: 148610 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate GFF248 HP15_247 (diaminopimelate decarboxylase)
to HMM TIGR01048 (lysA: diaminopimelate decarboxylase (EC 4.1.1.20))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01048.hmm # target sequence database: /tmp/gapView.12926.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01048 [M=417] Accession: TIGR01048 Description: lysA: diaminopimelate decarboxylase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-167 542.3 0.0 3.7e-167 542.0 0.0 1.0 1 lcl|FitnessBrowser__Marino:GFF248 HP15_247 diaminopimelate decarbo Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Marino:GFF248 HP15_247 diaminopimelate decarboxylase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 542.0 0.0 3.7e-167 3.7e-167 5 416 .. 7 411 .. 4 412 .. 0.99 Alignments for each domain: == domain 1 score: 542.0 bits; conditional E-value: 3.7e-167 TIGR01048 5 kdgeleiegvdlkelaeefgtPlYvydeetlrerlealkeafkaeeslvlYAvKAnsnlavlrllaeeGlgldvvs 80 +dgel++e++++ ++a++fgtP Yvy+++tl+++++a++ a +++ +lv+YAvKAnsnlavl++la++G+g+d+vs lcl|FitnessBrowser__Marino:GFF248 7 RDGELYAEDIPVSAIADRFGTPAYVYSRATLERHYRAYDDALRDHPHLVCYAVKANSNLAVLNVLARLGAGFDIVS 82 789************************************************************************* PP TIGR01048 81 gGEleralaAgvkaekivfsgngkseeeleaaleleiklinvdsveelelleeiakelgkkarvllRvnpdvdakt 156 GEler+l Ag +++++vfsg+gk+e e+++ale++++++nv+s +el++l+++a+elg ka+++lRvnpdvda t lcl|FitnessBrowser__Marino:GFF248 83 AGELERVLRAGGDPSRVVFSGVGKQEWEMKRALEVGVRCFNVESDTELDRLNAVAGELGVKAPISLRVNPDVDAGT 158 **************************************************************************** PP TIGR01048 157 heyisTGlkesKFGieveeaeeayelalkleslelvGihvHIGSqildlepfveaaekvvklleelkeegieleel 232 h+yisTGlke+KFGi+++ea ++y +a+ +++l++ G+++HIGSq++ ++pf +a+++v+ l+++l+++gi++++l lcl|FitnessBrowser__Marino:GFF248 159 HPYISTGLKENKFGIDIAEASQVYARAAGMPNLDVKGVDCHIGSQLTSVSPFLDALDRVLALIDSLADQGIHIHHL 234 **************************************************************************** PP TIGR01048 233 dlGGGlgisyeeeeeapdleeyaeklleklekeaelglklklilEpGRslvanagvlltrVesvKevesrkfvlvD 308 d+GGGlg++y++e+ +p++++y+++l+e+l + +kl+lilEpGRs++anag+l+trVe++K +e+r+f+++D lcl|FitnessBrowser__Marino:GFF248 235 DMGGGLGVTYNQEQ-PPQPSDYVKALAERLGD-----RKLELILEPGRSIAANAGILVTRVEFLKCTEHRNFAIID 304 *************9.**************999.....69************************************* PP TIGR01048 309 agmndliRpalYeayheiaalkrleeeetetvdvvGplCEsgDvlakdrelpeveeGdllavasaGAYgasmssnY 384 a+mndliRpalY+a++ i+++k ++ e++ +d+vGp+CE+gD+l+kdr+l + +Gdllav+saGAYg+ mssnY lcl|FitnessBrowser__Marino:GFF248 305 AAMNDLIRPALYSAWQAIIPVKPHQDGEEKAWDLVGPVCETGDFLGKDRHLRLQ-AGDLLAVRSAGAYGFVMSSNY 379 ***************************************************865.5******************** PP TIGR01048 385 nsrprpaevlveegkarlirrretledllale 416 n+r+rp e++v++++++++rrretled+la e lcl|FitnessBrowser__Marino:GFF248 380 NTRNRPPELMVDGDQVHVVRRRETLEDQLAPE 411 ****************************9976 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (417 nodes) Target sequences: 1 (416 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.23 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory