GapMind for Amino acid biosynthesis

 

Alignments for a candidate for tyrB in Marinobacter adhaerens HP15

Align Aromatic-amino-acid transaminase (EC 2.6.1.57) (characterized)
to candidate GFF1396 HP15_1363 histidinol-phosphate aminotransferase

Query= reanno::BFirm:BPHYT_RS14905
         (370 letters)



>FitnessBrowser__Marino:GFF1396
          Length = 374

 Score =  402 bits (1032), Expect = e-116
 Identities = 205/361 (56%), Positives = 264/361 (73%), Gaps = 3/361 (0%)

Query: 10  VRAIAPYIAGKPISEVAREFGLDEATIVKLASNENPLGMPESAQRAMAQAASELGRYPDA 69
           V+A++PY  GKPI E+ARE GL+ A I+KLASNENPLG  E A  A  +A SEL  YPD 
Sbjct: 13  VQALSPYQPGKPIEELARELGLNPAEIIKLASNENPLGPSEKALSAARRALSELCLYPDG 72

Query: 70  NAFELKAALSERYGVPADWVTLGNGSNDILEIAAHAFVEKGQSIVYAQYSFAVYALATQG 129
           N F LK ALS R  V  D +TLGNGSND+LE+    F +    +V++QY+FAVY L TQ 
Sbjct: 73  NGFNLKQALSTRLNVGMDQITLGNGSNDVLEVITRCFADADSEVVFSQYAFAVYPLVTQA 132

Query: 130 LGARAIVVPAVKYGHDLDAMLAAVSDDTRLIFVANPNNPTGTFIEGPKLEAFLDKVPRHV 189
           +GAR + VPA ++GHDLDAM AAV+D TRL+FVANPNNPTGT      +EAFL K+P +V
Sbjct: 133 IGARGVSVPAREWGHDLDAMAAAVTDRTRLVFVANPNNPTGTVHTAEAIEAFLKKIPENV 192

Query: 190 VVVLDEAYTEYLPQEKRYDSIAWVRRYPNLLVSRTFSKAFGLAGLRVGFAIAQPELTDLL 249
           +VVLDEAY EYL  ++  D +  + R+PNL+V RTFSKA+GLA LRVG++I+ P + D+L
Sbjct: 193 LVVLDEAYCEYLTGDQYPDGVELLARFPNLIVCRTFSKAWGLAALRVGYSISSPAIADIL 252

Query: 250 NRVRQPFNVNTLAQAAAIAALNDKAFLEKSAALNAQGYRRLTEAFDKLGLEYVPSDGNFV 309
           NRVRQPFNV+T+A +AA A L+D+ +L++S  +N  G R+L EAFD++GL Y+PS GNFV
Sbjct: 253 NRVRQPFNVDTVALSAATAVLDDEDYLQRSRDVNTAGLRQLQEAFDRMGLGYIPSAGNFV 312

Query: 310 LVRVGNDDAAGNRVNLELLKQGVIVRPVGNYGLPQWLRITIGLPEENEAFIAALERTLAA 369
            V VG + A G  V   LL  GVIVRP+  YG+P+ LR+++GLPEENE FI AL + L A
Sbjct: 313 AVEVG-EQAMG--VYQALLAHGVIVRPIAGYGMPRHLRVSVGLPEENERFIEALAQALTA 369

Query: 370 A 370
           +
Sbjct: 370 S 370


Lambda     K      H
   0.318    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 407
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 370
Length of database: 374
Length adjustment: 30
Effective length of query: 340
Effective length of database: 344
Effective search space:   116960
Effective search space used:   116960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory