Align Putative acetolactate synthase large subunit IlvX; ALS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; AHAS (uncharacterized)
to candidate GFF974 HP15_953 protein containing thiamine pyrophosphate enzyme, C-terminal TPP-binding / Thiamine pyrophosphate enzyme, N-terminal TPP binding region
Query= curated2:O53554 (515 letters) >FitnessBrowser__Marino:GFF974 Length = 552 Score = 523 bits (1348), Expect = e-153 Identities = 282/518 (54%), Positives = 356/518 (68%), Gaps = 11/518 (2%) Query: 2 NGAQALINTLVDGGVDVCFANPGTSEMHFVAALDAVPRMRGMLTLFEGVATGAADGYARI 61 NGAQAL+ TLVD GV+VCF+NPGTSEMHFVAALD P+MR +L LFEGVATGAADGYAR+ Sbjct: 3 NGAQALMKTLVDAGVEVCFSNPGTSEMHFVAALDDEPKMRAVLALFEGVATGAADGYARM 62 Query: 62 AGRPAAVLLHLGPGLGNGLANLHNARRARVPMVVVVGDHATYHKKYDAPLESDIDAVAGT 121 A +PAA LLHLG GLGNGLANLHNAR+ +VP++ +VGDHATYH KYDA L+SDI+ VA Sbjct: 63 ADKPAATLLHLGCGLGNGLANLHNARKGKVPVLNIVGDHATYHVKYDAQLQSDIETVARN 122 Query: 122 VS-GWVRRTEAAADVGADAEAAIAASRSG-SQIATLILPADVCWSDGAHAAAGV--PAQA 177 VS G+VR ++ + DA AIAA+R+ Q+ATLILPADV W +G +A + P Sbjct: 123 VSPGFVRTAKSTETLCQDAAEAIAAARTAPGQVATLILPADVSWGEGGVPSAPLAPPTPE 182 Query: 178 AAAPVDVGPVAGVLRSGEPAMMLIGGDATRGPGLTAAARIVQATGARWLCETFPTCLERG 237 A V +A +RSG+ +L+GG + R P + AAA++ +G L ETFPT +ERG Sbjct: 183 PADDATVEAIAKAIRSGKKTALLMGGHSLREPSMLAAAKLAAHSGVTLLAETFPTRMERG 242 Query: 238 AGIPAVERLAYFAEGAAAQLDGVKHLVLAGARSPVSFFAYPGMPSDLVPAGCEVHVLAEP 297 AG+P +ER+AY AE A QL ++ L+L +++PVSFFAYPG S LVP C+VH LA P Sbjct: 243 AGLPYIERIAYLAELATVQLTDIEQLILVDSKAPVSFFAYPGKKSYLVPDTCQVHTLAAP 302 Query: 298 GGAADALAA---LADEVAPGTVAP-VAGASRPQLPTGDLTSVSAADVVGALLPERAIVVD 353 D LA+ L D V P + RP P G LT+ VG L+PE AI+VD Sbjct: 303 D--QDILASLNKLNDAVGASQAQPKLQPEKRPGRPRGKLTAEKVCKAVGELMPENAIIVD 360 Query: 354 ESNTCGVLLPQATAGAPAHDWLTLTGGAIGYGIPAAVGAAVAAPDRPVLCLESDGSAMYT 413 E T ++L TAGAP HD +TLTGGAIG G+P AVGAAVA PDRPV+ L DG+AMYT Sbjct: 361 EGITSSLMLSVMTAGAPRHDMITLTGGAIGQGLPNAVGAAVACPDRPVIALIGDGTAMYT 420 Query: 414 ISGLWSQARENLDVTTVIYNNGAYDILRIELQRVGAGSDPGPKALDLLDISRPTMDFVKI 473 I LW+ ARE L+VT++I+NN +Y +L IEL+RVGA + G KA LD+ P ++F ++ Sbjct: 421 IQALWTMAREQLNVTSIIFNNASYSVLNIELERVGA-EEAGEKAKSQLDLRGPVINFAEM 479 Query: 474 AEGMGVPARRVTTCEEFADALRAAFAEPGPHLIDVVVP 511 A GMGV RV T EE A AL A PGPH+I+ ++P Sbjct: 480 ANGMGVHGVRVHTAEEMAKALEYAQRMPGPHVIEAMIP 517 Lambda K H 0.318 0.134 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 651 Number of extensions: 19 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 515 Length of database: 552 Length adjustment: 35 Effective length of query: 480 Effective length of database: 517 Effective search space: 248160 Effective search space used: 248160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory