GapMind for Amino acid biosynthesis

 

Protein 8501587 in Desulfovibrio vulgaris Miyazaki F

Annotation: FitnessBrowser__Miya:8501587

Length: 1078 amino acids

Source: Miya in FitnessBrowser

Candidate for 1 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine biosynthesis carB hi Carbamoyl-phosphate synthase large chain, chloroplastic; Carbamoyl-phosphate synthetase ammonia chain; Protein VENOSA 6; EC 6.3.5.5 (characterized) 59% 91% 1253
L-arginine biosynthesis carB hi carB: carbamoyl-phosphate synthase, large subunit (EC 6.3.5.5) (TIGR01369) 100% 1529.8

Sequence Analysis Tools

View 8501587 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MPKRTDLKRIMVIGSGPIVIGQACEFDYSGTQAVKALKEEGYEVVLVNSNPATIMTDPGL
ADRTYIEPIEPETVAAIIRKERPDALLPTLGGQTGLNTALALAAMGVLEECGVDLIGANK
QVIEKAESRELFREAMANIGLKVPASGIARTMEDVRRLGTEMPFPLIIRPAFTMGGTGGG
IAYNMEDLEEIAARGLAASIKHEVMIEQSVLGWKEFEMEVMRDKADNCVIICSIENFDPM
GVHTGDSITVAPAQTLTDVEYQMMRDASIAIMREIGVETGGSNVQFGINPQNGDMVVIEM
NPRVSRSSALASKATGFPIAKIAAKLAVGYTLDEIPNDITRETMASFEPSIDYCVTKIPR
FTFEKFPGAKDELTTSMKSVGEAMSIGRTFKESLQKGLRSLEVGAPGLGSAFRCKGPERE
DIMRKLRTPNSRRIFYVRHAMLDGMTVEEIHEATAIDPWFLRQMKDLVDMEAELRDFALG
NAMTVDNAELVALMRRAKEYGFSDRQLAEMWKRPESDVRTLRKQMGIAPTYYLVDTCASE
FEAYTPYYYSTYETGHEVAVEDRKKVIILGGGPNRIGQGIEFDYCCCHASFALKEMGVQA
IMVNSNPETVSTDYDTSDRLYFEPLTFEDVMNIIETEKPDGVVVQFGGQTPLNLAVPLMR
AGVPILGTSPDSIDRAEDRERFQALLQKLGLRQPANATVMSLPEARSAAARIGYPTVVRP
SYVLGGRAMEIVYDEEQLAEYFANSVGEKPKHPILIDKFLESAIEVDVDALSDGTDVYVA
GIMEHIEEAGIHSGDSACVIPPHTLPEAIVNEIARQTVELARELRVVGLMNIQFAVKDGL
IYILEVNPRASRTAPFVSKATAVPLPRLATQIMLGATLKELDPWSMRRTGYFSVKESVFP
FNRFPGVDILLGPEMRSTGEVMGIASSFEEAYLKGQLAGGQRLPQSGKIFISVNDRDKLL
ITEVASMFADLGFEVLATSGTAKLLREGGVPATSVHKVYEGRPNIVDFIKNGDIALVLNT
ASGKRTVQDSKSIRQATLMYGVPYSTTVSGAKAIAQAIRASRCCGVNVKSLQEYYGTN

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory