GapMind for Amino acid biosynthesis

 

Aligments for a candidate for aroA in Desulfovibrio vulgaris Miyazaki F

Align 3-phosphoshikimate 1-carboxyvinyltransferase; EC 2.5.1.19 (characterized, see rationale)
to candidate 8501011 DvMF_1748 3-phosphoshikimate 1-carboxyvinyltransferase (RefSeq)

Query= uniprot:Q72EV5_DESVH
         (439 letters)



>lcl|FitnessBrowser__Miya:8501011 DvMF_1748 3-phosphoshikimate
           1-carboxyvinyltransferase (RefSeq)
          Length = 450

 Score =  620 bits (1599), Expect = 0.0
 Identities = 315/445 (70%), Positives = 349/445 (78%), Gaps = 7/445 (1%)

Query: 2   EPVVVTAPPSKSLSHRALIGAALAAGESTVEHVLESRDLTCTADILRAAGAHIERRAPGS 61
           E V V APPSKS+SHR LIGAALAAG+S VEHVLESRD+  TADILRAAGA IER+  G 
Sbjct: 6   ETVTVAAPPSKSVSHRMLIGAALAAGDSVVEHVLESRDIERTADILRAAGARIERQGDGR 65

Query: 62  YLVRGVAGTPAGGFEAPVSCDVHESGTTCRLLTAVLAAGMGRFRIHGAPRMHERPIGELT 121
           + V GVAGTPAGGF+APVSCDVHESGTTCRLLTAVLAAG G+FRIHGAPRMHERPIGEL 
Sbjct: 66  FAVSGVAGTPAGGFDAPVSCDVHESGTTCRLLTAVLAAGKGQFRIHGAPRMHERPIGELV 125

Query: 122 DALASLGVQIAFEARAGFPPLVIDAAGMRGGEVSIGMDESSQYLSGLLLAAPLTQGMTVN 181
           D L   GV++ +E R G PPL+ID+ GM GG  +IG+ ESSQYLSGLLLAAPLT G+T+ 
Sbjct: 126 DVLRGRGVRVTYEGREGCPPLLIDSDGMSGGSTAIGLGESSQYLSGLLLAAPLTSGLTIE 185

Query: 182 VAGKSVVSWPYVGLTLQTLEDFGIDFTVETRDAVDAPWGAVDWRSLHEAVPGLVRFRVRP 241
           V+G  VVSWPYV LTLQ LEDFGI F VETR+     W A DWR L E  PGLVRF V P
Sbjct: 186 VSGDKVVSWPYVALTLQALEDFGIGFRVETRETPRGKWQADDWRMLREVRPGLVRFVVSP 245

Query: 242 GMYRAGSYRVEGDWSGASYFLAAGAVGPRPVRVEGLRVDSLQGDRALLDILTAMGADIRP 301
           G+YRAG+YRVEGDWS ASYFLAAGAVGPRPVRV GLRVDSLQGDRA+LDIL  MGA    
Sbjct: 246 GVYRAGNYRVEGDWSNASYFLAAGAVGPRPVRVAGLRVDSLQGDRAMLDILGRMGARFER 305

Query: 302 GEGHVVVHPAPLHGVEVDMGHCPDLVPTVAAVAAFATGRTVVRNVAHLRIKESDRIAAPV 361
            +  VVV P+ L GVEVDMGHCPDLVPTVAA AAFA G T +RNVAHLRIKE DR++AP 
Sbjct: 306 ADNGVVVAPSSLTGVEVDMGHCPDLVPTVAATAAFAQGVTTIRNVAHLRIKECDRLSAPA 365

Query: 362 GELRRAGVNAEERADGLVVQGGALATEPGTL-------FSAHGDHRMAMSLALLGLGGVD 414
            ELR+AGV  EE  DGL+V G   +  P  +       F ++GDHRMAMSLALLG  GV 
Sbjct: 366 AELRKAGVRVEELDDGLIVHGSLRSGGPAPVIDEKVMPFLSYGDHRMAMSLALLGFAGVH 425

Query: 415 VRLDDPSCVSKSFPHFWELWEKVRA 439
           V LDDP+CV+KSFPHFW  WEKVRA
Sbjct: 426 VVLDDPACVAKSFPHFWNEWEKVRA 450


Lambda     K      H
   0.320    0.137    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 715
Number of extensions: 30
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 439
Length of database: 450
Length adjustment: 32
Effective length of query: 407
Effective length of database: 418
Effective search space:   170126
Effective search space used:   170126
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory