GapMind for Amino acid biosynthesis

 

Aligments for a candidate for cmutase in Desulfovibrio vulgaris Miyazaki F

Align Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 (characterized)
to candidate 8501012 DvMF_1749 prephenate dehydratase (RefSeq)

Query= SwissProt::P27603
         (365 letters)



>lcl|FitnessBrowser__Miya:8501012 DvMF_1749 prephenate dehydratase
           (RefSeq)
          Length = 418

 Score =  259 bits (663), Expect = 7e-74
 Identities = 150/361 (41%), Positives = 212/361 (58%), Gaps = 12/361 (3%)

Query: 4   ADQLKALRVRIDSLDERILDLISERARCAQEVARVKTASWPKAEEAVFYRPEREAWVLKH 63
           A +L  +R  ID LD  +L+L++ RA  + EV R+K       +  + ++P RE  VL++
Sbjct: 57  AGRLGQIRHEIDGLDSDLLNLLNRRASLSLEVGRIKAD-----DAGIVFKPFREREVLEN 111

Query: 64  IMELNKGPLDNEEMARLFREIMSSCLALEQPLRVAYLGPEGTFSQAAALKHFGHSVISKP 123
           +M  N GPL NE +  ++REI+SS  +L++P +VAYLGPEGTFS  A ++  G +V   P
Sbjct: 112 LMAANGGPLPNEHLRSIWREILSSSRSLQRPQKVAYLGPEGTFSYFAGVEFLGKAVEYMP 171

Query: 124 MAAIDEVFREVVAGAVNFGVVPVENSTEGAVNHTLDSFLEHDIVICGEVELRIHHHLLVG 183
              +D VFR V       GVVP+ENS  G V  +LD FL H++ I  E+  RI H LL  
Sbjct: 172 QKDLDGVFRAVHDRQCELGVVPLENSLHGTVGQSLDLFLSHEVFIQSELFCRISHCLLTT 231

Query: 184 ETTKTDRITRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNADAAKRVKSEWNSAAIAGDM 243
           ET+  D +T +YSH Q LAQC  WL    P    +   S A AA+RV  E  +AAI    
Sbjct: 232 ETSLAD-VTTVYSHPQPLAQCGGWLRQALPGARIIPADSTASAARRVGGEKGAAAIGHRS 290

Query: 244 AAQLYGLSKLAEKIEDRPVNSTRFLIIGSQEVPPTGDDKTSIIVSMRNKPGALHELLMPF 303
            A L GL+ LA  IED+P N TRF++IG       G DKTS++ S+ ++PGAL E+L   
Sbjct: 291 LAALLGLNILARGIEDQPDNWTRFVVIGPAPAGQPGTDKTSMLFSVPDRPGALAEVLNLL 350

Query: 304 HSNGIDLTRIETRPSRSGKWTYVFFIDC---MGHHQDPLIKNVLEKIGHEAVALKVLGSY 360
              GI++ ++E+RP R  KW YVFF+D    +G+     + + L ++ H    L++LGSY
Sbjct: 351 AREGINMKKLESRPLRGEKWKYVFFVDVECDLGNEDYGRVVHELRRLCH---TLRILGSY 407

Query: 361 P 361
           P
Sbjct: 408 P 408


Lambda     K      H
   0.319    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 343
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 365
Length of database: 418
Length adjustment: 31
Effective length of query: 334
Effective length of database: 387
Effective search space:   129258
Effective search space used:   129258
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

Align candidate 8501012 DvMF_1749 (prephenate dehydratase (RefSeq))
to HMM PF01817 (CM_2)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/PF01817.21.hmm
# target sequence database:        /tmp/gapView.8254.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       CM_2  [M=75]
Accession:   PF01817.21
Description: Chorismate mutase type II
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
    2.1e-24   72.0   0.0    4.2e-24   71.1   0.0    1.5  1  lcl|FitnessBrowser__Miya:8501012  DvMF_1749 prephenate dehydratase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Miya:8501012  DvMF_1749 prephenate dehydratase (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   71.1   0.0   4.2e-24   4.2e-24       1      74 [.      64     140 ..      64     141 .. 0.97

  Alignments for each domain:
  == domain 1  score: 71.1 bits;  conditional E-value: 4.2e-24
                                       HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCTSGSS-HHHHHHHHHHHHH...HHS-HHHHHHHHHHHHHHHHHHH CS
                              CM_2   1 RkeIdeiDdeileLLaeRaelakevgeyKkeeglpvydpeReaevlerlee...galdeeaierifreiisasralq 74 
                                       R+eId +D+++l+LL++Ra l+ evg++K++ +  v+ p Re+evle+l++   g+l++e +++i+rei+s sr+lq
  lcl|FitnessBrowser__Miya:8501012  64 RHEIDGLDSDLLNLLNRRASLSLEVGRIKADDAGIVFKPFREREVLENLMAangGPLPNEHLRSIWREILSSSRSLQ 140
                                       99***************************999989****************98888********************9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (75 nodes)
Target sequences:                          1  (418 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 10.02
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory