Alignments for a candidate for argJ in Desulfovibrio vulgaris Miyazaki F

GapMind for Amino acid biosynthesis

Alignments for a candidate for argJ in Desulfovibrio vulgaris Miyazaki F

Align Glutamate N-acetyltransferase (EC 2.3.1.35) (characterized)
to candidate 8502162 DvMF_2872 bifunctional ornithine acetyltransferase/N-acetylglutamate synthase protein (RefSeq)

Query= reanno::DvH:206250
         (393 letters)



>FitnessBrowser__Miya:8502162
          Length = 412

 Score =  567 bits (1462), Expect = e-166
 Identities = 286/391 (73%), Positives = 318/391 (81%)

Query: 3   ASPKGFRFATVSAGFRKEARPDLALIVSDTPATAAGVFTTNRFQAAPVVVARENLSARPV 62
           A+PKGFRFAT  AGFRK  R DLAL+VSDTPA AA VFT N FQAAPV+VAR+ L+  P 
Sbjct: 22  AAPKGFRFATAGAGFRKAGRDDLALVVSDTPAVAAAVFTKNLFQAAPVLVARDTLAHAPK 81

Query: 63  ARAVVINSGQANACTGDEGMTNCRTTLDLVGKACGIPAAEVLPASTGVIGAQLHMDKWRE 122
           ARAV+INSGQANACTGDEG+ NCR TL+LV  A G+   ++LPASTGVIGAQL MDKW  
Sbjct: 82  ARAVLINSGQANACTGDEGLANCRATLELVAAATGLAPQDILPASTGVIGAQLAMDKWAA 141

Query: 123 AAPRLAAALGQNTHHDFARAIMTTDAFPKVAERELAIAGTTVRLVGMAKGAGMICPNMAT 182
           A P LAA LG     DFA+AIMTTDAFPK A R + ++G TV LVGMAKGAGMICPNMAT
Sbjct: 142 AVPALAADLGNKGPEDFAKAIMTTDAFPKFASRTVELSGGTVTLVGMAKGAGMICPNMAT 201

Query: 183 MLSVVLCDAAVTPEAWQRLFLDAVDRTFNRVTVDGDTSTNDTVFGLANGASGVTVEGEDL 242
           ML+ VLCDA V P AWQ LF DAVD TFNRVTVDGDTSTNDTVFGLANGASGV   G DL
Sbjct: 202 MLATVLCDADVEPAAWQALFRDAVDATFNRVTVDGDTSTNDTVFGLANGASGVAARGADL 261

Query: 243 AKLGEALTDVLARLAYMLVQDGEGATKVMRVKVSGAVDDAEAEAVARTVGHSQLVKTAMY 302
             LG ALT VL  +AYMLV+DGEGATKV+ + V+GA DDA+AE  ARTVGHSQLVKTAMY
Sbjct: 262 TVLGAALTGVLGDVAYMLVKDGEGATKVIHINVAGARDDADAELAARTVGHSQLVKTAMY 321

Query: 303 GRDANWGRIVAAVGRSGASFKAEDVVVTLCGVELFRNGQPTDLDFDTLLREPLKGRDVTV 362
           GRDANWGRIVAA+GRSGA+F    V+V+LCGVELFR GQPT+LDFD LL+EPL GRD+ V
Sbjct: 322 GRDANWGRIVAALGRSGATFNPAAVIVSLCGVELFRGGQPTNLDFDALLKEPLAGRDIVV 381

Query: 363 DIELGAGTGHYELLASDLTHDYVNCNADYRS 393
           +I LG G G Y LLASDLTHDY++CNADYRS
Sbjct: 382 NIMLGNGPGSYRLLASDLTHDYISCNADYRS 412


Lambda     K      H
   0.318    0.132    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 577
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 412
Length adjustment: 31
Effective length of query: 362
Effective length of database: 381
Effective search space:   137922
Effective search space used:   137922
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis