Align acetohydroxyacid synthase subunit B (EC 2.2.1.6) (characterized)
to candidate 8499376 DvMF_0153 acetolactate synthase, large subunit, biosynthetic type (RefSeq)
Query= metacyc::MONOMER-18810 (585 letters) >FitnessBrowser__Miya:8499376 Length = 562 Score = 605 bits (1559), Expect = e-177 Identities = 313/565 (55%), Positives = 402/565 (71%), Gaps = 7/565 (1%) Query: 18 EMIGAEILVHALAEEGVEYVWGYPGGAVLYIYDELHKQTKFEHILVRHEQAAVHAADGYA 77 E+ GA IL+ L EGV+ ++GYPGGAV+ IYDEL + HILVRHEQAAVHAADG+A Sbjct: 2 ELNGARILLECLKREGVDVLFGYPGGAVIDIYDELPRHD-IRHILVRHEQAAVHAADGFA 60 Query: 78 RATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVVITGNVPTHAIGQDAFQECDTVGIT 137 RA+GKVGV LVTSGPG TN VTGIATAY DSIPMVV+TG VPT IG DAFQE D VGIT Sbjct: 61 RASGKVGVCLVTSGPGATNTVTGIATAYADSIPMVVLTGQVPTPLIGNDAFQEVDIVGIT 120 Query: 138 RPIVKHNFLVKDVRDLAATIKKAFFIAATGRPGPVVVDIPKDVSRNACKYEYPKSIDMRS 197 RP KHN+LVKDVRDLA +++AF++A +GRPGPV+VD+PKDV + ++ +P+ + +RS Sbjct: 121 RPCTKHNYLVKDVRDLARVLRQAFYLARSGRPGPVLVDLPKDVMQAKTEFVWPEDVKLRS 180 Query: 198 YNPVNKGHSGQIRKAVALLQGAERPYIYTGGGVVLANASDELRQLAALTGHPVTNTLMGL 257 YNP + + Q+R+AV L A+RP + GGGV++++AS+EL LA PVT+TLMGL Sbjct: 181 YNPTYRPNLNQLRRAVEALIEAKRPLFFAGGGVIMSDASEELGWLARTLSIPVTSTLMGL 240 Query: 258 GAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGNPAHFTSQARKIIHI 317 GAFPG ++GM+GMHGTY N A+ N D ++A+GARFDDRV G + F S AR IIHI Sbjct: 241 GAFPGDDPLWLGMIGMHGTYAGNKAVNNADCIVAVGARFDDRVTGRLSAFASGAR-IIHI 299 Query: 318 DIDPSSISKRVKVDIPIVGNVKDVLQELIAQIKASDIKPKREALA--KWWEQIEQWRSVD 375 DIDP+SI K V+VDIP+VG+ + L I +I + + K A A W Q+ WR+ Sbjct: 300 DIDPTSIRKNVQVDIPVVGDCRKALAG-IREIATTRLAEKDWATAHEPWLTQVSAWRTEQ 358 Query: 376 CLKYDRSSEIIKPQYVVEKIWELTKGDAFICSDVGQHQMWAAQFYKFDEPRRWINSGGLG 435 L Y ++ IKPQ VVE I+ +T+GDA + ++VGQ+QMW AQF+ F +PR + SGGLG Sbjct: 359 PLTYVKNGS-IKPQQVVETIFSITRGDAIVTTEVGQNQMWTAQFFTFRKPRTLLTSGGLG 417 Query: 436 TMGVGLPYAMGIKKAFPEKEVVTITGEGSIQMCIQELSTCLQYDTPVKICSLNNGYLGMV 495 TMG G P A+G + AFP+K V+ + G+GSIQM IQEL+T + P+KI LNNGYLGMV Sbjct: 418 TMGYGFPAAIGAQFAFPDKLVIDMAGDGSIQMNIQELATAVCNKLPIKIVILNNGYLGMV 477 Query: 496 RQWQEIEYDNRYSHSYMDALPDFVKLAEAYGHVGMRVEKTSDVEPALREAFRLKDRTVFL 555 RQWQE+ Y Y + M+A PDFVKLAEAYG G R+ + ++E LR AF T + Sbjct: 478 RQWQELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITEAGELESTLRAAF-ASPHTAVI 536 Query: 556 DFQTDPTENVWPMVQAGKGISEMLL 580 D + + ENV+PMV AG + EMLL Sbjct: 537 DVRVEREENVYPMVPAGAALDEMLL 561 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 866 Number of extensions: 33 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 585 Length of database: 562 Length adjustment: 36 Effective length of query: 549 Effective length of database: 526 Effective search space: 288774 Effective search space used: 288774 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate 8499376 DvMF_0153 (acetolactate synthase, large subunit, biosynthetic type (RefSeq))
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.5617.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-256 837.1 0.0 3.6e-256 836.9 0.0 1.0 1 lcl|FitnessBrowser__Miya:8499376 DvMF_0153 acetolactate synthase, Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Miya:8499376 DvMF_0153 acetolactate synthase, large subunit, biosynthetic type (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 836.9 0.0 3.6e-256 3.6e-256 1 554 [. 3 560 .. 3 562 .] 0.97 Alignments for each domain: == domain 1 score: 836.9 bits; conditional E-value: 3.6e-256 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydalydselehilvrheqaaahaadGyarasGkvGvvlatsGPGatn 77 l+ga+il+e lk+egv+++fGyPGGav++iyd+l +++hilvrheqaa+haadG+arasGkvGv+l+tsGPGatn lcl|FitnessBrowser__Miya:8499376 3 LNGARILLECLKREGVDVLFGYPGGAVIDIYDELPRHDIRHILVRHEQAAVHAADGFARASGKVGVCLVTSGPGATN 79 579************************************************************************** PP TIGR00118 78 lvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeilkeafeiastGrPGPvl 154 +vtgiatay+ds+P+vvltGqv+t liG+dafqe+di+Git+p+tkh++lvk+++dl+++l++af++a +GrPGPvl lcl|FitnessBrowser__Miya:8499376 80 TVTGIATAYADSIPMVVLTGQVPTPLIGNDAFQEVDIVGITRPCTKHNYLVKDVRDLARVLRQAFYLARSGRPGPVL 156 ***************************************************************************** PP TIGR00118 155 vdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllvGgGviiaeaseelkelaerlkipv 231 vdlPkdv++a++e+ +e+v+l++y+pt +++ +q+++a+e++ +ak+P+ ++GgGvi+++aseel la +l ipv lcl|FitnessBrowser__Miya:8499376 157 VDLPKDVMQAKTEFVWPEDVKLRSYNPTYRPNLNQLRRAVEALIEAKRPLFFAGGGVIMSDASEELGWLARTLSIPV 233 ***************************************************************************** PP TIGR00118 232 tttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGarfddrvtgnlakfapeakiihididPaeigknv 308 t+tl+GlGafp d+pl lgm+GmhGt++ n av++ad+++avGarfddrvtg l+ fa+ a+iihididP++i knv lcl|FitnessBrowser__Miya:8499376 234 TSTLMGLGAFPGDDPLWLGMIGMHGTYAGNKAVNNADCIVAVGARFDDRVTGRLSAFASGARIIHIDIDPTSIRKNV 310 ***************************************************************************** PP TIGR00118 309 kvdipivGdakkvleellkklkee......ekkeke.WlekieewkkeyilkldeeeesikPqkvikelskllkdea 378 +vdip+vGd++k l+ + + ++++ + +e Wl +++ w++e++l++ +++sikPq+v++++ +++++a lcl|FitnessBrowser__Miya:8499376 311 QVDIPVVGDCRKALAGIREIATTRlaekdwA-TAHEpWLTQVSAWRTEQPLTY-VKNGSIKPQQVVETIFSITRGDA 385 **************98876655544444533.33346*************876.56799****************** PP TIGR00118 379 ivttdvGqhqmwaaqfyktkkprkfitsgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydi 455 ivtt+vGq+qmw+aqf++++kpr+ +tsgGlGtmG+G+Paa+Ga+ a p++ v++++Gdgs+qmn+qel+t+v ++ lcl|FitnessBrowser__Miya:8499376 386 IVTTEVGQNQMWTAQFFTFRKPRTLLTSGGLGTMGYGFPAAIGAQFAFPDKLVIDMAGDGSIQMNIQELATAVCNKL 462 ***************************************************************************** PP TIGR00118 456 pvkivilnnellGmvkqWqelfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldv 532 p+kivilnn +lGmv+qWqelfy++ y +t++++ +pdfvklaeayG++g ri++ ele+ l+ a++s + +++dv lcl|FitnessBrowser__Miya:8499376 463 PIKIVILNNGYLGMVRQWQELFYQRNYCSTCMEA-QPDFVKLAEAYGAEGYRITEAGELESTLRAAFASPHTAVIDV 538 *********************************5.****************************************** PP TIGR00118 533 evdkeeevlPmvapGagldelv 554 +v++ee+v+Pmv+ Ga+lde++ lcl|FitnessBrowser__Miya:8499376 539 RVEREENVYPMVPAGAALDEML 560 *********************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (562 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 7.90 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory