Align glutamate N-acetyltransferase/amino-acid acetyltransferase; EC 2.3.1.35 2.3.1.1 (characterized)
to candidate Dsui_0226 Dsui_0226 glutamate N-acetyltransferase/amino-acid acetyltransferase
Query= CharProtDB::CH_000559 (406 letters) >FitnessBrowser__PS:Dsui_0226 Length = 413 Score = 425 bits (1093), Expect = e-123 Identities = 230/415 (55%), Positives = 290/415 (69%), Gaps = 11/415 (2%) Query: 1 MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAGSTVGAVFTTNRFCAAP 60 M VN E L + G+ L TA+AG++K DLT+IA+ GSTV VFT NRFCAAP Sbjct: 1 MPVNYATPAPEALFPVAGVRLGTAEAGIRKANRRDLTVIALDPGSTVAGVFTQNRFCAAP 60 Query: 61 VHIAKSHLFDEDGVRALVINTGNANAGTGAQGRIDALAVCAAAARQIGC-KPNQVMPFST 119 V + + HL E +RALVINTG ANAGTG G A+ CA A +G + Q++PFST Sbjct: 61 VQVCRKHLAGESEIRALVINTGIANAGTGETGLQAAMQTCATLAGLLGLDEDRQILPFST 120 Query: 120 GVILEPLPADKIIAALPK----MQPAFWNEAARAIMTTDTVPKAASREGKVGDQHTVRAT 175 GVILEPLP D++ A LP ++ W+ AA IMTTDTV KAASR+ ++G + +V T Sbjct: 121 GVILEPLPVDRLQAGLPAAIADLKADNWHAAAHGIMTTDTVAKAASRKVEIGGK-SVTIT 179 Query: 176 GIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETFNTITVDGDTSTNDSFVI 235 GI+KG+GMI PNMATMLGF+ATDA + Q +L+ + +E AD++FN ITVDGDTSTNDSF++ Sbjct: 180 GISKGAGMIKPNMATMLGFLATDAGIPQHLLEQVAKEAADQSFNCITVDGDTSTNDSFIV 239 Query: 236 IATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDGEGATKFITVRVENAKTCDE 295 +ATG+ S I + ADP +A LK + ++++ELAQAIVRDGEGATKFITV VE K +E Sbjct: 240 MATGQ-SGLRIKDGADPAWAPLKAAIVAVSIELAQAIVRDGEGATKFITVAVEGGKDLEE 298 Query: 296 ARQAAYAAARSPLVKTAFFASDPNLGKRLAAIGYADVADLDTDLVEMYL----DDILVAE 351 R+ YA SPLVKTAFFASDPNLG+ LAAIGYA + DLD + ++L D +LVAE Sbjct: 299 CRKVGYAIGHSPLVKTAFFASDPNLGRILAAIGYAGITDLDVGQIRVWLASGSDAVLVAE 358 Query: 352 HGGRAASYTEAQGQAVMSKDEITVRIKLHRGQAAATVYTCDLSHGYVSINADYRS 406 GGRAA+Y E G +M EITVR+ L RG A +Y+CD S+ YV INADYRS Sbjct: 359 KGGRAAAYKEEDGSRIMKNAEITVRVDLARGTAQGCIYSCDFSYDYVKINADYRS 413 Lambda K H 0.317 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 440 Number of extensions: 15 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 406 Length of database: 413 Length adjustment: 31 Effective length of query: 375 Effective length of database: 382 Effective search space: 143250 Effective search space used: 143250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory