Alignments for a candidate for aroE in Dechlorosoma suillum PS

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroE in Dechlorosoma suillum PS

Align shikimate dehydrogenase (NADP+) (EC 1.1.1.25) (characterized)
to candidate Dsui_0443 Dsui_0443 shikimate 5-dehydrogenase

Query= BRENDA::Q88RQ5
         (274 letters)



>FitnessBrowser__PS:Dsui_0443
          Length = 271

 Score =  308 bits (788), Expect = 1e-88
 Identities = 170/270 (62%), Positives = 192/270 (71%), Gaps = 4/270 (1%)

Query: 2   DQYVVFGNPIGHSKSPLIHRLFAEQTGQDLEYATLLAPLDEFSDCARGFFKQGS-GGNVT 60
           D+Y VFGNPIGHSKSPLIH LFA    QD+ Y  LLAPLD F+   R F   G  G NVT
Sbjct: 3   DRYAVFGNPIGHSKSPLIHSLFAVACAQDMSYEALLAPLDGFAAALRAFADGGGRGANVT 62

Query: 61  VPFKEEAFRLCDSLTPRARRAGAVNTLSKLADGTLQGDNTDGAGLVRDLTVNAGVELAGK 120
           VPFKEEAFRL DSLTPRA RAGAVNTL  L  G + GDNTDGAGLV+DL VN  + LAGK
Sbjct: 63  VPFKEEAFRLADSLTPRAARAGAVNTLV-LEGGRILGDNTDGAGLVQDLQVNQSLPLAGK 121

Query: 121 RILILGAGGAVRGVLEPILAHKPQSLVIANRTVEKAEQLAREFDELGPVVASGFAWLQ-E 179
            IL+LGAGGA RG L P+LA  P  L IANRT  KA  LAR+F +LGPV   G+  L  +
Sbjct: 122 AILLLGAGGASRGALAPLLAAGPCRLHIANRTAAKAADLARDFADLGPVSGGGYGELAGQ 181

Query: 180 PVDVIINATSASLAGELPPIADSLVEAGRTVCYDMMYGKEPTPFCQWATKLGAAKVLDGL 239
             DV+INATSASLAGELPP+   +   G  + YDMMYG+  TPF  +A   GA  ++DGL
Sbjct: 182 GFDVVINATSASLAGELPPLPPGIFNGG-ALAYDMMYGRGETPFLAFARLEGATHLVDGL 240

Query: 240 GMLAEQAAEAFFIWRGVRPDTAPVLAELRR 269
           GML EQAAEAF +WRGVRP TAPVL  LR+
Sbjct: 241 GMLVEQAAEAFQLWRGVRPATAPVLDRLRQ 270


Lambda     K      H
   0.319    0.137    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 310
Number of extensions: 11
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 274
Length of database: 271
Length adjustment: 25
Effective length of query: 249
Effective length of database: 246
Effective search space:    61254
Effective search space used:    61254
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate Dsui_0443 Dsui_0443 (shikimate 5-dehydrogenase)
to HMM TIGR00507 (aroE: shikimate dehydrogenase (EC 1.1.1.25))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00507.hmm
# target sequence database:        /tmp/gapView.21540.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00507  [M=270]
Accession:   TIGR00507
Description: aroE: shikimate dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
    4.4e-86  274.3   0.0    4.9e-86  274.2   0.0    1.0  1  lcl|FitnessBrowser__PS:Dsui_0443  Dsui_0443 shikimate 5-dehydrogen


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__PS:Dsui_0443  Dsui_0443 shikimate 5-dehydrogenase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  274.2   0.0   4.9e-86   4.9e-86       3     265 ..       5     268 ..       3     271 .] 0.97

  Alignments for each domain:
  == domain 1  score: 274.2 bits;  conditional E-value: 4.9e-86
                         TIGR00507   3 lgviGnpikhSksplihnaalkqlgleleYlafeveieelekalsgikalglkGvnvTvPfKeevlellDeieesak 79 
                                       ++v+Gnpi hSksplih  ++    +++ Y a+ ++++ +  al ++   g +G+nvTvPfKee+++l+D ++++a+
  lcl|FitnessBrowser__PS:Dsui_0443   5 YAVFGNPIGHSKSPLIHSLFAVACAQDMSYEALLAPLDGFAAALRAFADGGGRGANVTVPFKEEAFRLADSLTPRAA 81 
                                       9**************************************************************************** PP

                         TIGR00507  80 ligavNTlkledgklvgynTDgiGlvssLek.lsklksekrvliiGAGGaakavaleLlka.dkeviiaNRtvekae 154
                                        +gavNTl+le+g+++g+nTDg Glv++L+  +s + ++k +l++GAGGa+++++ +Ll a ++++ iaNRt +ka 
  lcl|FitnessBrowser__PS:Dsui_0443  82 RAGAVNTLVLEGGRILGDNTDGAGLVQDLQVnQSLPLAGKAILLLGAGGASRGALAPLLAAgPCRLHIANRTAAKAA 158
                                       ******************************98888888*********************9989************** PP

                         TIGR00507 155 elaerlqelgeilalsleevelkkvdliinatsaglsgeideaevkaellkegklvvDlvynpletpllkeakkkg. 230
                                       +la  ++ lg +      e+  + +d++inatsa+l ge+  +++++++++ g+l++D++y  +etp+l+ a+ +g 
  lcl|FitnessBrowser__PS:Dsui_0443 159 DLARDFADLGPVSGGGYGELAGQGFDVVINATSASLAGEL--PPLPPGIFNGGALAYDMMYGRGETPFLAFARLEGa 233
                                       ****************************************..**********************************9 PP

                         TIGR00507 231 tkvidGlgMlvaQaalsFelwtgvepdvekvfeal 265
                                       t+ +dGlgMlv+Qaa +F+lw+gv p    v ++l
  lcl|FitnessBrowser__PS:Dsui_0443 234 THLVDGLGMLVEQAAEAFQLWRGVRPATAPVLDRL 268
                                       9*************************998887776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (271 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 10.80
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis