Align 3-isopropylmalate dehydratase small subunit; EC 4.2.1.33 (characterized, see rationale)
to candidate Dsui_3199 Dsui_3199 3-isopropylmalate dehydratase, small subunit
Query= uniprot:Q845W4 (216 letters) >FitnessBrowser__PS:Dsui_3199 Length = 212 Score = 327 bits (837), Expect = 1e-94 Identities = 154/210 (73%), Positives = 174/210 (82%) Query: 1 MEKFTVHTGVVAPLDRENVDTDAIIPKQFLKSIKRTGFGPNAFDEWRYLDHGEPGQDNSK 60 M+ FT G+VAPLDR NVDTDAIIPKQFLKSIKR+GFGPNAFDEWRY+DHGEPG DNSK Sbjct: 1 MQAFTKLDGLVAPLDRNNVDTDAIIPKQFLKSIKRSGFGPNAFDEWRYMDHGEPGMDNSK 60 Query: 61 RPLNPDFVLNQPRYQGASILVTRKNFGCGSSREHAPWALQQYGFRAIIAPSFADIFFNNC 120 RPLNP+FVLNQ RYQGAS+L+TR NFGCGSSREHAPWAL YGFR +IA SFADIFFNNC Sbjct: 61 RPLNPNFVLNQQRYQGASVLLTRSNFGCGSSREHAPWALLDYGFRVVIAESFADIFFNNC 120 Query: 121 FKNGLLPIVLTEQQVDHLINETVAFNGYQLTIDLEAQVVRTPDGRDYPFEITAFRKYCLL 180 FKNG+LPIVL + ++D L T G++L +DLE Q V PDG PFE+ AFR+ CLL Sbjct: 121 FKNGILPIVLPKTEIDALFGLTEYTPGFKLVVDLEQQKVVRPDGHAIPFEVDAFRRECLL 180 Query: 181 NGFDDIGLTLRHADKIRQFEAERLAKQPWL 210 NG+DDIGLTLRHADKI+ FE R A+QPWL Sbjct: 181 NGWDDIGLTLRHADKIKAFEERRRAEQPWL 210 Lambda K H 0.322 0.141 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 236 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 216 Length of database: 212 Length adjustment: 22 Effective length of query: 194 Effective length of database: 190 Effective search space: 36860 Effective search space used: 36860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 45 (21.9 bits)
Align candidate Dsui_3199 Dsui_3199 (3-isopropylmalate dehydratase, small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00171.hmm # target sequence database: /tmp/gapView.5498.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00171 [M=188] Accession: TIGR00171 Description: leuD: 3-isopropylmalate dehydratase, small subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.3e-85 270.1 0.0 6.2e-85 269.8 0.0 1.0 1 lcl|FitnessBrowser__PS:Dsui_3199 Dsui_3199 3-isopropylmalate dehy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PS:Dsui_3199 Dsui_3199 3-isopropylmalate dehydratase, small subunit # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 269.8 0.0 6.2e-85 6.2e-85 1 188 [] 1 196 [. 1 196 [. 0.94 Alignments for each domain: == domain 1 score: 269.8 bits; conditional E-value: 6.2e-85 TIGR00171 1 mkefkkltGlvvpldkanvdtdaiipkqflkkikrtGfgkhlfyewrylde..kGkep.....npefvlnvpqyqga 70 m+ f kl+Glv+pld+ nvdtdaiipkqflk ikr Gfg ++f ewry+d+ G ++ np+fvln+++yqga lcl|FitnessBrowser__PS:Dsui_3199 1 MQAFTKLDGLVAPLDRNNVDTDAIIPKQFLKSIKRSGFGPNAFDEWRYMDHgePGMDNskrplNPNFVLNQQRYQGA 77 899*********************************************9852234332222359************* PP TIGR00171 71 sillarenfGcGssrehapwalkdyGfkviiapsfadifynnsfkngllpirlseeeveellalvk.nkglkltvdl 146 s+ll+r nfGcGssrehapwal dyGf+v+ia sfadif+nn+fkng+lpi l++ e++ l+ l + +g kl vdl lcl|FitnessBrowser__PS:Dsui_3199 78 SVLLTRSNFGCGSSREHAPWALLDYGFRVVIAESFADIFFNNCFKNGILPIVLPKTEIDALFGLTEyTPGFKLVVDL 154 *****************************************************************999********* PP TIGR00171 147 eaqkvkdsegkvysfeidefrkhcllnGldeigltlqkedei 188 e+qkv +g+ + fe+d+fr++cllnG+d+igltl++ d+i lcl|FitnessBrowser__PS:Dsui_3199 155 EQQKVVRPDGHAIPFEVDAFRRECLLNGWDDIGLTLRHADKI 196 ****************************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (188 nodes) Target sequences: 1 (212 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 6.63 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory