Alignments for a candidate for metY in Dechlorosoma suillum PS

GapMind for Amino acid biosynthesis

Alignments for a candidate for metY in Dechlorosoma suillum PS

Align O-acetylhomoserine aminocarboxypropyltransferase (EC 2.5.1.49) (characterized)
to candidate Dsui_2039 Dsui_2039 OAH/OAS sulfhydrylase

Query= BRENDA::P94890
         (442 letters)



>FitnessBrowser__PS:Dsui_2039
          Length = 428

 Score =  501 bits (1291), Expect = e-146
 Identities = 245/424 (57%), Positives = 309/424 (72%), Gaps = 9/424 (2%)

Query: 16  ETIALHGGQEPDPTTTSRAVPLYQTTSYVFKDTDHAARLFGLQEFGNIYTRLMNPTTDVL 75
           +T++LH GQ PD  T SRA P+YQT +YVF   +HAA LF LQ FGN+Y+RL NPT  VL
Sbjct: 9   DTLSLHAGQVPDAATGSRATPIYQTAAYVFDSPEHAASLFNLQTFGNVYSRLSNPTVAVL 68

Query: 76  EKRVAALEGGVAALATASGQSAEMLALLNIVEAGQEIVASSSLYGGTYNLLHYTFPKLGI 135
           E+R+AALEGG AA+A ASG +A+M+ALLN+ EAG EIVA+ +LYGGT+  L   F KLGI
Sbjct: 69  EERIAALEGGKAAVAVASGMAAQMVALLNLCEAGDEIVAARTLYGGTHTQLDVNFRKLGI 128

Query: 136 KVHFVDQSDPENFRKASNDKTRAFYAETLGNPKLDTLDIAAVSKVAKEVGVPLVIDNTMP 195
              FVD  DPENF KA   +T+  YAET+GNP L+ LDI AV+++A   G+PL +DNT  
Sbjct: 129 NTIFVDSDDPENFAKAITPRTKVIYAETMGNPGLNVLDIEAVARIANAAGLPLFVDNTFA 188

Query: 196 SPYLVNPLKHGADIVVHSLTKFLGGHGTSIGGIIIDGGSFNWGNGKFKNFTEPDPSYHGL 255
           SPYL  P + GA IVVHS TKF+GGHGT++GGII++ G F W NGKF   TEP P YHG+
Sbjct: 189 SPYLCRPFEWGAAIVVHSATKFIGGHGTTLGGIIVESGKFPWDNGKFATMTEPSPGYHGV 248

Query: 256 KFWEVFGKFEPFGGVNIAFILKARVQGLRDLGPAISPFNAWQILQGVETLPLRMERHSGN 315
           KF+E FG        N  F  K R++GLR  GPA+SPFNA+ +LQGVETL LRM+RH  N
Sbjct: 249 KFYETFG--------NFGFTAKCRMEGLRTFGPALSPFNAFLLLQGVETLSLRMDRHCSN 300

Query: 316 ALKVAEFLQKHPKIEWVNYPGLSTDKNYATAKKYHERGLFGAIVGFEIKGGVEKAKKFID 375
           AL +A  L+ HP++EWVNYPGL + K +  A+KY  +G  GA++ F IKGG    ++FID
Sbjct: 301 ALAIARHLEAHPQVEWVNYPGLPSSKYHDLAQKYLPKGA-GAVLTFGIKGGAAAGQEFID 359

Query: 376 GLELFSLLANIGDAKSLAIHPASTTHQQLTGPEQISAGVTPGFVRLSVGLENIDDILVDL 435
            +E  S LANIGDAK+L IHPASTTH+Q+T  +Q +AGV P  +RLSVGLE +DDIL D+
Sbjct: 360 SVEFLSHLANIGDAKTLVIHPASTTHRQMTPEQQTAAGVPPDLIRLSVGLETLDDILWDI 419

Query: 436 EEAL 439
           ++AL
Sbjct: 420 DQAL 423


Lambda     K      H
   0.317    0.137    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 556
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 442
Length of database: 428
Length adjustment: 32
Effective length of query: 410
Effective length of database: 396
Effective search space:   162360
Effective search space used:   162360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis