Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate 5207684 Shew_0206 argininosuccinate lyase (RefSeq)
Query= ecocyc::ARGSUCCINLYA-MONOMER (457 letters) >FitnessBrowser__PV4:5207684 Length = 453 Score = 602 bits (1551), Expect = e-176 Identities = 308/450 (68%), Positives = 352/450 (78%) Query: 1 MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKALVTVGVLTAEEQAQLEE 60 MALWGGRF+Q + FK FNDSL DYRL E+DIVGS+AW+ A+ VG+L+ +E L++ Sbjct: 1 MALWGGRFSQESSALFKLFNDSLPVDYRLVEEDIVGSIAWADAITGVGILSQQECQSLKQ 60 Query: 61 ALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKKLHTGRSRNDQVATDLKLW 120 AL LL +V+ +P+ IL S AED HS+VE LI KVG LGKKLHTGRSRNDQVATDLKLW Sbjct: 61 ALQELLAEVKDQPETILASGAEDTHSFVEQALIAKVGDLGKKLHTGRSRNDQVATDLKLW 120 Query: 121 CKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRAQPVTFAHWCLAYVEMLARDE 180 CK LL QL++AL+ A+ DAVMPGYTHLQRAQPV F HWCLAYVEM RD Sbjct: 121 CKKEGKALLELLAQLKAALLSLAERELDAVMPGYTHLQRAQPVAFGHWCLAYVEMFERDI 180 Query: 181 SRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGFASATRNSLDSVSDRDHVLELLS 240 SRLQD L+RLD PLG GALAGTAY +DR LA LGFAS T NSLDSVSDRDHV+EL S Sbjct: 181 SRLQDCLQRLDTCPLGTGALAGTAYPMDRYALAKSLGFASPTLNSLDSVSDRDHVIELCS 240 Query: 241 AAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSGSSLMPQKKNPDALELIRGKCGRVQ 300 AAI M+HLSR AEDLIFFN+GEAGF+EL D+VTSGSSLMPQKKNPDALELIRGK GRV Sbjct: 241 DAAISMMHLSRMAEDLIFFNSGEAGFIELDDQVTSGSSLMPQKKNPDALELIRGKTGRVY 300 Query: 301 GALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDTWLDCLHMAALVLDGIQVKRPRCQEAA 360 G+L G++ T+K LPLAYNKDMQEDKEGLFD +D+W CL MAALVL G++V R AA Sbjct: 301 GSLMGILTTMKALPLAYNKDMQEDKEGLFDVMDSWSICLEMAALVLSGLKVNRENTLSAA 360 Query: 361 QQGYANATELADYLVAKGVPFREAHHIVGEAVVEAIRQGKPLEDLPLSELQKFSQVIDED 420 QQGYANATELADYLVAKG+PFREAHH+VGE VVEAI Q KP+E+ L+ LQ F+ VI ED Sbjct: 361 QQGYANATELADYLVAKGMPFREAHHVVGEVVVEAIAQKKPIEEFELASLQVFASVIQED 420 Query: 421 VYPILSLQSCLDKRAAKGGVSPQQVAQAIA 450 VY L++ SCL KR A GG S QV A+A Sbjct: 421 VYQCLTIDSCLAKREALGGTSLTQVKAALA 450 Lambda K H 0.318 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 570 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 457 Length of database: 453 Length adjustment: 33 Effective length of query: 424 Effective length of database: 420 Effective search space: 178080 Effective search space used: 178080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate 5207684 Shew_0206 (argininosuccinate lyase (RefSeq))
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00838.hmm # target sequence database: /tmp/gapView.28844.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00838 [M=455] Accession: TIGR00838 Description: argH: argininosuccinate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-191 622.2 0.0 3.7e-191 621.9 0.0 1.0 1 lcl|FitnessBrowser__PV4:5207684 Shew_0206 argininosuccinate lyas Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5207684 Shew_0206 argininosuccinate lyase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 621.9 0.0 3.7e-191 3.7e-191 1 448 [. 3 450 .. 3 452 .. 0.99 Alignments for each domain: == domain 1 score: 621.9 bits; conditional E-value: 3.7e-191 TIGR00838 1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevkeg.klelevd 77 lwggR+++e + ++ fn+sl D++l+e+Di gsia++ a++ +gil+++e + l++aL+el evk++ + l + lcl|FitnessBrowser__PV4:5207684 3 LWGGRFSQESSALFKLFNDSLPVDYRLVEEDIVGSIAWADAITGVGILSQQECQSLKQALQELLAEVKDQpETILASG 80 7*******************************************************************9989999*** PP TIGR00838 78 aeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalvekAekevetlmpgytHL 155 aeD H+ vE+ li+kvg d+gkklhtgrsRnDqvatdl+l+++++ k l+e l +l++al+ +Ae+e +++mpgytHL lcl|FitnessBrowser__PV4:5207684 81 AEDTHSFVEQALIAKVG-DLGKKLHTGRSRNDQVATDLKLWCKKEGKALLELLAQLKAALLSLAERELDAVMPGYTHL 157 *****************.************************************************************ PP TIGR00838 156 qrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidrellaelLgFdavvensldavsdRDfi 233 qrAqP+ ++H++lay+em+erD++Rl+d l+R++++PlG+gAlagt++++dr +la+ LgF++ + nsld+vsdRD++ lcl|FitnessBrowser__PV4:5207684 158 QRAQPVAFGHWCLAYVEMFERDISRLQDCLQRLDTCPLGTGALAGTAYPMDRYALAKSLGFASPTLNSLDSVSDRDHV 235 ****************************************************************************** PP TIGR00838 234 iEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgssimPqKKnpDvaEliRgktgrviGnltglltilKal 311 iEl s+aa+ m+hlsr+aE+li+f+s E+gf+el+d+v+sgss+mPqKKnpD++EliRgktgrv+G+l+g+lt++Kal lcl|FitnessBrowser__PV4:5207684 236 IELCSDAAISMMHLSRMAEDLIFFNSGEAGFIELDDQVTSGSSLMPQKKNPDALELIRGKTGRVYGSLMGILTTMKAL 313 ****************************************************************************** PP TIGR00838 312 PlaYnkDlqEdkealfdalktveellevvtgllkelkvnkerleeaakknfalatdlAdylvrkGvPFReaheivGev 389 PlaYnkD+qEdke+lfd ++ ++ +le+++++l+ lkvn+e++ aa++++a+at+lAdylv+kG+PFReah++vGev lcl|FitnessBrowser__PV4:5207684 314 PLAYNKDMQEDKEGLFDVMDSWSICLEMAALVLSGLKVNRENTLSAAQQGYANATELADYLVAKGMPFREAHHVVGEV 391 ****************************************************************************** PP TIGR00838 390 vakaiekGkkleeltleelqklsekleedvlevldleeavekrdakGGtakeevekaie 448 v++ai++ k +ee l+ lq + + ++edv++ l++ ++++kr+a GGt+ ++v+ a++ lcl|FitnessBrowser__PV4:5207684 392 VVEAIAQKKPIEEFELASLQVFASVIQEDVYQCLTIDSCLAKREALGGTSLTQVKAALA 450 *******************************************************9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (453 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.48 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory