Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EPSP phospholyase; EC 4.2.3.5 (characterized)
to candidate 5209968 Shew_2416 chorismate synthase (RefSeq)
Query= SwissProt::P12008 (361 letters) >FitnessBrowser__PV4:5209968 Length = 364 Score = 552 bits (1422), Expect = e-162 Identities = 275/359 (76%), Positives = 311/359 (86%) Query: 1 MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR 60 M+GN+IGQ F VTTFGESHG ALGCI+DG PPG+ + EAD+QHDLDRRRPGTSRYTT RR Sbjct: 1 MSGNSIGQNFVVTTFGESHGKALGCIIDGCPPGLAIDEADMQHDLDRRRPGTSRYTTARR 60 Query: 61 EPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDY 120 EPDQVKILSGVFEG TTGTSIGL+IENTDQRSQDYS IKD FRPGHADYTY+QKYGLRDY Sbjct: 61 EPDQVKILSGVFEGQTTGTSIGLVIENTDQRSQDYSNIKDQFRPGHADYTYQQKYGLRDY 120 Query: 121 RGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIKDWSQVEQNPFFC 180 RGGGRSSARETAMRVAAGA+AKKYL + GIEI+G L+Q+G I +I D+ QVE N FF Sbjct: 121 RGGGRSSARETAMRVAAGAVAKKYLKQVHGIEIKGYLSQLGPICAEILDFDQVEHNAFFF 180 Query: 181 PDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAHALMSINAV 240 PD K++ LDE MR LKK GDSIGAKV+VVA+ VP GLGEPVFDRLDADIAHALM INAV Sbjct: 181 PDAAKLEQLDEYMRELKKSGDSIGAKVSVVATNVPVGLGEPVFDRLDADIAHALMGINAV 240 Query: 241 KGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGILGGISSGQQIIAHMALKPTSSITV 300 KGVEIGDGF VV +GS++RD ++ +GF SNHAGGILGGISSGQ I+AH+A+KPTSSI+V Sbjct: 241 KGVEIGDGFAVVNQKGSEHRDLMSPEGFASNHAGGILGGISSGQPIVAHIAMKPTSSISV 300 Query: 301 PGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQRAQNADVKTDIP 359 PG ++ GE E++TKGRHDPCVGIRAVPIAEAMLAIVLMDHLLR RAQN +V++ P Sbjct: 301 PGESMTVQGERAEVVTKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRHRAQNMNVESITP 359 Lambda K H 0.319 0.138 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 468 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 361 Length of database: 364 Length adjustment: 29 Effective length of query: 332 Effective length of database: 335 Effective search space: 111220 Effective search space used: 111220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate 5209968 Shew_2416 (chorismate synthase (RefSeq))
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00033.hmm # target sequence database: /tmp/gapView.27334.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00033 [M=351] Accession: TIGR00033 Description: aroC: chorismate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.8e-145 469.6 0.5 3.2e-145 469.5 0.5 1.0 1 lcl|FitnessBrowser__PV4:5209968 Shew_2416 chorismate synthase (R Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5209968 Shew_2416 chorismate synthase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 469.5 0.5 3.2e-145 3.2e-145 1 350 [. 10 350 .. 10 351 .. 0.97 Alignments for each domain: == domain 1 score: 469.5 bits; conditional E-value: 3.2e-145 TIGR00033 1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDeveilsGvfeGkTtGaPiallikN 78 + +ttfGeSHgkalg+iidG+P+gl ++e+d+q++l+rRrpg+sr+t+ r+E D+v+ilsGvfeG+TtG+ i l+i+N lcl|FitnessBrowser__PV4:5209968 10 FVVTTFGESHGKALGCIIDGCPPGLAIDEADMQHDLDRRRPGTSRYTTARREPDQVKILSGVFEGQTTGTSIGLVIEN 87 569*************************************************************************** PP TIGR00033 79 kdvrskdyedikelpRPgHadytylkKYgikdregggrsSaReTaarvaaGavakklLketagieivayvvklgevel 156 +d+rs+dy++ik+++RPgHadyty++KYg++d++gggrsSaReTa+rvaaGavakk+Lk+ +giei++y+++lg +++ lcl|FitnessBrowser__PV4:5209968 88 TDQRSQDYSNIKDQFRPGHADYTYQQKYGLRDYRGGGRSSARETAMRVAAGAVAKKYLKQVHGIEIKGYLSQLGPICA 165 *****************************************************************************9 PP TIGR00033 157 eeesakeiskerldkspvrcpdaeaekemeeeidkakkdgdsvGgvvevvvsnvpvglGeplfdkldaelasallsin 234 e ++ +++++ +++pda + +++ e++ ++kk+gds+G++v+vv++nvpvglGep+fd+lda +a+al++in lcl|FitnessBrowser__PV4:5209968 166 EILDFD-----QVEHNAFFFPDAAKLEQLDEYMRELKKSGDSIGAKVSVVATNVPVGLGEPVFDRLDADIAHALMGIN 238 965544.....69999************************************************************** PP TIGR00033 235 AvKgveiGdGFeaasvrGseanDelvleddkirrktnnsGGieGGitnGedirvriavKpiptikkplktvdletkek 312 AvKgveiGdGF+ ++++Gse+ D + + + +n+ GGi+GGi+ G++i+ +ia+Kp+++i+ p ++++++++ + lcl|FitnessBrowser__PV4:5209968 239 AVKGVEIGDGFAVVNQKGSEHRDLMS--PE--GFASNHAGGILGGISSGQPIVAHIAMKPTSSISVPGESMTVQGERA 312 ***********************554..33..589**************************************99999 PP TIGR00033 313 akatkgRhDpcvvpravpvvEamvalvladallekras 350 +tkgRhDpcv +ravp++Eam+a+vl+d+ll++ra+ lcl|FitnessBrowser__PV4:5209968 313 EVVTKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRHRAQ 350 9999*******************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (351 nodes) Target sequences: 1 (364 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.97 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory