Align fused aspartate kinase/homoserine dehydrogenase 2 (EC 2.7.2.4; EC 1.1.1.3) (characterized)
to candidate 5208005 Shew_0521 bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq)
Query= ecocyc::ASPKINIIHOMOSERDEHYDROGII-MONOMER (810 letters) >FitnessBrowser__PV4:5208005 Length = 797 Score = 572 bits (1473), Expect = e-167 Identities = 342/804 (42%), Positives = 479/804 (59%), Gaps = 26/804 (3%) Query: 14 LHKFGGSSLADVKCYLRVAGIMAEYSQPDDMMVVSAAGSTTNQLINWLKLSQTDRLSAHQ 73 LHKFGGSSLAD CY RVA I+ + DD++VVSAAG +TN L L+L TD+L + Sbjct: 6 LHKFGGSSLADADCYRRVAHILLTHGHSDDLIVVSAAGKSTNFLYRLLELCDTDQLWQEE 65 Query: 74 VQQTLRRYQCDLISGLLPAEEADSLISAFVSDLERLAALLD-SGINDAVYAEVVGHGEVW 132 +Q L YQ +LI LL E+A SL +D +L +LL +ND +VV GE W Sbjct: 66 LQ-VLVSYQQNLIEQLLSNEQARSLRERLATDKSQLTSLLSLDELNDYQRNQVVSFGERW 124 Query: 133 SARLMSAVLNQQGLPAAWLDAREFLRAERAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTG 192 S+ L++A+L + G+ A +DAR L A+ A PQ+ G S +Q LL HP +RLV+TG Sbjct: 125 SSCLLAALLRESGVAATDVDARTLLVADEGAVPQIRLGESRQKVQALLEAHPQERLVITG 184 Query: 193 FISRNNAGETVLLGRNGSDYSATQIGALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLL 252 FI N G+T+LLGRNGSD+SAT + +LA + RVTIW+DV GV++ADP ++ DA LL + Sbjct: 185 FICANEQGDTLLLGRNGSDFSATLLASLADIERVTIWTDVEGVFNADPNRINDAKLLKSM 244 Query: 253 RLDEASELARLAAPVLHARTLQPVSGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVT 312 L EA+ LA L +PVLH RTLQP+ +E+ L +R SY P T I +S A +VT Sbjct: 245 SLTEANRLAHLGSPVLHNRTLQPLFDTEVSLAVRSSYAPHTDFTLIAPK-SSSASAPVVT 303 Query: 313 SHDDVCLIEFQVPASQDFKLAHKEIDQILK---RAQVRPLAV-GVHNDRQLLQFCYTSEV 368 S V L + E+ Q+L +A + PLA + + R L F + ++ Sbjct: 304 SLSSVSLFSLTISV---------ELPQLLACFAQAGLTPLAYWSLAHGRAELAFTHENQK 354 Query: 369 ADSALKILDEAGLP-GELRLRQGLALVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSD 427 A+ + L EL+LR L LVA+V A + L+ F + L + + Sbjct: 355 QAQAIFEAKRSELGIDELQLRDDLGLVALVSADAS---LYRRGFSRLLSREAHPLC---N 408 Query: 428 DGISLVAVLRTGPTESLIQGLHQSVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSAR 487 DG+SLV ++ L Q +H+ KRIG++L G GNIG W++LF R + L+ Sbjct: 409 DGLSLVTLVPKAQVNLLTQKVHRRCAGPRKRIGVLLLGIGNIGEAWIDLFKRARPVLNKE 468 Query: 488 TGFEFVLAGVVDSRRSLLSYDGLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLD 547 L G++ S+++LLS +D S F + LF ++A D+L+ LD Sbjct: 469 LEVSVELVGLLSSKKALLSETDIDLSSWQQAFEERGTGWQYTHLFEQLQALNCDELIALD 528 Query: 548 VTASQQLADQYLDFASHGFHVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGL 607 ++AS L QY +F + G H++SANKLAG+ Y+++ W YNA+ GAGL Sbjct: 529 ISASADLTLQYPEFFARGIHIVSANKLAGSGPLAFYQELKQQLGNRRLFWRYNASCGAGL 588 Query: 608 PINHTVRDLIDSGDTILSISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDD 667 P+ H + DL +SGD+I ++ GIFSGTL WLF +D PF+ELV +A G+TEPDPRDD Sbjct: 589 PVQHALNDLHNSGDSIEAVGGIFSGTLCWLFEHYDAKKPFSELVLEAKGLGITEPDPRDD 648 Query: 668 LSGKDVMRKLVILAREAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLE 727 LSG+D+ RKL+ILARE GY+IE D + +ESLVP +D F + EL+ +M+Q+ + Sbjct: 649 LSGRDMQRKLLILAREIGYDIELDDIELESLVPTKLAEIPLDDFIKRMAELDGEMLQQYQ 708 Query: 728 AAREMGLVLRYVARFDANG---KARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLV 784 AA E VLRYVA D+ KA+VG++ V HP A+L P DNVF I S +Y+DNPL+ Sbjct: 709 AAAEQNKVLRYVASLDSEQGQLKAKVGIQWVDRSHPYANLTPGDNVFVIRSDFYQDNPLI 768 Query: 785 IRGPGAGRDVTAGAIQSDINRLAQ 808 IRGPGAGR+VTA A+QSD+ ++ + Sbjct: 769 IRGPGAGREVTAAAVQSDLAQICR 792 Lambda K H 0.320 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1467 Number of extensions: 76 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 810 Length of database: 797 Length adjustment: 41 Effective length of query: 769 Effective length of database: 756 Effective search space: 581364 Effective search space used: 581364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 55 (25.8 bits)
Align candidate 5208005 Shew_0521 (bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq))
to HMM TIGR00657 (aspartate kinase (EC 2.7.2.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00657.hmm # target sequence database: /tmp/gapView.6164.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00657 [M=442] Accession: TIGR00657 Description: asp_kinases: aspartate kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.6e-66 210.4 0.0 4.3e-66 209.7 0.0 1.2 1 lcl|FitnessBrowser__PV4:5208005 Shew_0521 bifunctional aspartate Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5208005 Shew_0521 bifunctional aspartate kinase II/homoserine dehydrogenase II (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 209.7 0.0 4.3e-66 4.3e-66 6 394 .. 7 393 .. 4 433 .. 0.78 Alignments for each domain: == domain 1 score: 209.7 bits; conditional E-value: 4.3e-66 TIGR00657 6 qKFGGtSvgnverikkvakivkkekekgnqvvVVvSAmagvTdaLvelaekvsseee.keliekirekhlealeelas 82 KFGG+S+++++ ++va+i++ + +++ ++VvSA +++T+ L l e ++ ++ +e+++++ + + + +e+l s lcl|FitnessBrowser__PV4:5208005 7 HKFGGSSLADADCYRRVAHILLTHGHSD--DLIVVSAAGKSTNFLYRLLELCDTDQLwQEELQVLVSYQQNLIEQLLS 82 6*******************99998555..78999*******************999988888888888888888885 PP TIGR00657 83 qalkeklkallekeleevkk........ereldlilsvGEklSaallaaaleelgvkavsllgaeagiltdsefgrAk 152 ++ + l++ l++ ++ + + ++ ++s+GE+ S+ llaa+l+e gv a + ++a+ +++d+ lcl|FitnessBrowser__PV4:5208005 83 NEQARSLRERLATDKSQLTSllsldelnDYQRNQVVSFGERWSSCLLAALLRESGVAA-TDVDARTLLVADEGAVPQI 159 556666655555555555556788899899*************************633.3445555555554433333 PP TIGR00657 153 vleeikterleklleeg..iivvvaGFiGatekgeittLGRGGSDltAallAaalkAdeveiytDVdGiytaDPrivp 228 ++++++++lle+ v++GFi a+e+g++ +LGR+GSD++A+llA++ + ++v i+tDV+G++ aDP+ ++ lcl|FitnessBrowser__PV4:5208005 160 -RLGESRQKVQALLEAHpqERLVITGFICANEQGDTLLLGRNGSDFSATLLASLADIERVTIWTDVEGVFNADPNRIN 236 .4555666666666666556699******************************************************* PP TIGR00657 229 eArrldeisyeEalELaslGakvLhprtlepamrakipivvkstfnpeaeGTlivakskseeepavkalsldknqalv 306 +A++l+++s Ea La+lG+ vLh+rtl+p+ ++ + v+s++ p+++ Tli +ks+s ++p v++ls+ +l+ lcl|FitnessBrowser__PV4:5208005 237 DAKLLKSMSLTEANRLAHLGSPVLHNRTLQPLFDTEVSLAVRSSYAPHTDFTLIAPKSSSASAPVVTSLSS---VSLF 311 *********************************************************99888888887765...4556 PP TIGR00657 307 svsgttmkpgilaevfgalaeakvnvdlil.qsssetsisfvvdkedadkakellkkkvkeekaleevevekklalvs 383 s+ ++ +++ + a+a+ + + ++ ++f+ ++ ++a+++ ++ ++e + e+++ ++l lv+ lcl|FitnessBrowser__PV4:5208005 312 SLTISVEL----PQLLACFAQAGLTPLAYWsLAHGRAELAFTHEN--QKQAQAIF-EAKRSELGIDELQLRDDLGLVA 382 55555554....566778888887776666133344445555555..55555554.346788999999********** PP TIGR00657 384 lvGagmksapg 394 lv+a+ + + lcl|FitnessBrowser__PV4:5208005 383 LVSADASLYRR 393 **999876544 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (442 nodes) Target sequences: 1 (797 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 17.44 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory