Align 3-isopropylmalate dehydrogenase (EC 1.1.1.85) (characterized)
to candidate 5211055 Shew_3471 3-isopropylmalate dehydrogenase (RefSeq)
Query= BRENDA::D2YZL2 (364 letters) >FitnessBrowser__PV4:5211055 Length = 364 Score = 647 bits (1669), Expect = 0.0 Identities = 318/362 (87%), Positives = 343/362 (94%) Query: 1 MSYQIAVLAGDGIGPEVMAEARKVLAAVEKRFDLSIEYSEYDVGGAAIDNHGCPLPEATL 60 MSYQ+AVLAGDGIGPEVMAEARKVL AVE+RF L IEYSEYDVGGAAID+HGCPLPE+TL Sbjct: 1 MSYQVAVLAGDGIGPEVMAEARKVLKAVEERFSLDIEYSEYDVGGAAIDHHGCPLPESTL 60 Query: 61 KGCEAADAVLFGSVGGPKWEHLPPNDQPERGALLPLRGHFELFCNMRPAKLHPGLEHMSP 120 KGCE ADA+LFGSVGGPKWEHLPPNDQPERGALLPLRGHFELFCNMRPAKLH GLEHMSP Sbjct: 61 KGCEEADAILFGSVGGPKWEHLPPNDQPERGALLPLRGHFELFCNMRPAKLHVGLEHMSP 120 Query: 121 LRSDISEKGFDILCVRELTGGIYFGKPKGRQGEGENEEAFDTMRYSRKEIRRIAKIAFES 180 LRSDIS +GFD+LCVRELTGGIYFGKPKGRQGEGE EEAFDTMRYSR+E+RRIAKIAFE+ Sbjct: 121 LRSDISAQGFDVLCVRELTGGIYFGKPKGRQGEGEQEEAFDTMRYSRREVRRIAKIAFEA 180 Query: 181 AQGRRKKVTSVDKANVLACSVLWREVVEEVAKDYPDVELEHIYIDNATMQLLRRPNEFDV 240 A+GRRKKVTSVDKANVLACSVLWREVVEEVAK++PDV LEHIYIDNATMQLLRRP +FDV Sbjct: 181 ARGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPDVALEHIYIDNATMQLLRRPFDFDV 240 Query: 241 MLCSNLFGDIVSDEIAMLTGSMGLLASISMNSQGFGMYEPAGGSAPDIAGQGIANPVAQI 300 MLCSNLFGDI+SDEIAMLTGSMGLL+S+S+NS GFG+YEPAGGSAPDIAGQGIANP+AQI Sbjct: 241 MLCSNLFGDIISDEIAMLTGSMGLLSSVSLNSDGFGLYEPAGGSAPDIAGQGIANPIAQI 300 Query: 301 LSAALLLRHSLKLEDAALAIEAAVSKALSDGYLTCELLPASERSQAKSTSQMGDYIAQAI 360 LSAALLLRHSLK E AA AIE AV+KALSDGYLT ELLPA +R +AKST +MGDYIAQA+ Sbjct: 301 LSAALLLRHSLKEEAAAKAIEDAVAKALSDGYLTGELLPADQRDKAKSTREMGDYIAQAV 360 Query: 361 AE 362 E Sbjct: 361 RE 362 Lambda K H 0.317 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 609 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 364 Length of database: 364 Length adjustment: 29 Effective length of query: 335 Effective length of database: 335 Effective search space: 112225 Effective search space used: 112225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate 5211055 Shew_3471 (3-isopropylmalate dehydrogenase (RefSeq))
to HMM TIGR00169 (leuB: 3-isopropylmalate dehydrogenase (EC 1.1.1.85))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00169.hmm # target sequence database: /tmp/gapView.8007.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00169 [M=349] Accession: TIGR00169 Description: leuB: 3-isopropylmalate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.9e-160 519.6 0.0 2.1e-160 519.4 0.0 1.0 1 lcl|FitnessBrowser__PV4:5211055 Shew_3471 3-isopropylmalate dehy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__PV4:5211055 Shew_3471 3-isopropylmalate dehydrogenase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 519.4 0.0 2.1e-160 2.1e-160 1 347 [. 4 354 .. 4 356 .. 0.97 Alignments for each domain: == domain 1 score: 519.4 bits; conditional E-value: 2.1e-160 TIGR00169 1 kiavLpGDgiGpevvaealkvLkaveerfelklefeealiGGaaidatgePlpeetlkackeadavLlgavGGpkWdn 78 ++avL+GDgiGpev+aea kvLkaveerf l++e++e+++GGaaid++g Plpe tlk+c+eada+L+g+vGGpkW++ lcl|FitnessBrowser__PV4:5211055 4 QVAVLAGDGIGPEVMAEARKVLKAVEERFSLDIEYSEYDVGGAAIDHHGCPLPESTLKGCEEADAILFGSVGGPKWEH 81 59**************************************************************************** PP TIGR00169 79 lprdvrPek.gLLklrkeldlfanLrPaklfksLeklsplkeeiv.kgvDlvvvreLtgGiYfGepkereeaeeekka 154 lp++ +Pe+ +LL+lr +++lf+n+rPakl+ +Le++spl+++i +g+D+++vreLtgGiYfG+pk+r++++e+++a lcl|FitnessBrowser__PV4:5211055 82 LPPNDQPERgALLPLRGHFELFCNMRPAKLHVGLEHMSPLRSDISaQGFDVLCVRELTGGIYFGKPKGRQGEGEQEEA 159 *********88*********************************77******************************** PP TIGR00169 155 ldtekYtkeeieriarvafelarkrrkkvtsvDkanvLessrlWrktveeiakeyPdvelehlyiDnaamqLvksPeq 232 +dt++Y+++e++ria++afe+ar rrkkvtsvDkanvL+ s lWr++vee+ake+Pdv+leh+yiDna+mqL+++P lcl|FitnessBrowser__PV4:5211055 160 FDTMRYSRREVRRIAKIAFEAARGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPDVALEHIYIDNATMQLLRRPFD 237 ****************************************************************************** PP TIGR00169 233 ldvvvtsnlfGDilsDeasvitGslGlLPsaslsskglalfepvhgsapdiagkgianpiaailsaalllryslnlee 310 +dv+++snlfGDi+sDe +++tGs+GlL s sl+s+g++l+ep++gsapdiag+gianpia+ilsaalllr+sl+ e lcl|FitnessBrowser__PV4:5211055 238 FDVMLCSNLFGDIISDEIAMLTGSMGLLSSVSLNSDGFGLYEPAGGSAPDIAGQGIANPIAQILSAALLLRHSLKEEA 315 ****************************************************************************** PP TIGR00169 311 aaeaieaavkkvleegkrtedla..seattavstkevee 347 aa+aie+av+k+l++g+ t +l ++ +a st+e+++ lcl|FitnessBrowser__PV4:5211055 316 AAKAIEDAVAKALSDGYLTGELLpaDQRDKAKSTREMGD 354 ******************999861144456667777766 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (349 nodes) Target sequences: 1 (364 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.01 # Mc/sec: 10.57 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory