GapMind for Amino acid biosynthesis

 

Aligments for a candidate for lysT in Pedobacter sp. GW460-11-11-14-LB5

Align Probable methanogen homoaconitase large subunit; HACN; EC 4.2.1.114; Homoaconitate hydratase (uncharacterized)
to candidate CA265_RS15830 CA265_RS15830 3-isopropylmalate dehydratase large subunit

Query= curated2:O27668
         (428 letters)



>lcl|FitnessBrowser__Pedo557:CA265_RS15830 CA265_RS15830
           3-isopropylmalate dehydratase large subunit
          Length = 470

 Score =  221 bits (562), Expect = 5e-62
 Identities = 147/442 (33%), Positives = 225/442 (50%), Gaps = 62/442 (14%)

Query: 30  VDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPA--------NTIGAA 81
           +D  + H+ TSP   + F  +  RG P  V+ P++ V   DHNVP           +   
Sbjct: 27  IDTHLIHEVTSP---QAFDGLRKRGLP--VFRPKQTVATADHNVPTLNQLLPIKEELSRY 81

Query: 82  EFQRVTR---EFARE-QGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFG 137
           +   +T+   EF  E  G+ + FQ   GI H + PE G   PG  +V  DSHT T+GAFG
Sbjct: 82  QVDMLTKNCAEFGVELYGLGHPFQ---GIVHVIGPELGITLPGKTMVCGDSHTSTHGAFG 138

Query: 138 AFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATY 197
           A A G+G + +  VFAT       P+ M+IEV GE    V AKD+IL+II +I   G T 
Sbjct: 139 AIAFGIGTSQVEQVFATQCLLQSKPKTMKIEVNGELGKGVGAKDIILYIIAKISAAGGTG 198

Query: 198 RSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYVRAR----TGRE---- 249
             +E+ G  IE++ +  RMTICNM++EMGA+ G++ P++ T DY++ R     G E    
Sbjct: 199 YFIEYAGSAIEALSMEARMTICNMSIEMGARGGLIAPDQTTFDYIKGREFAPAGEEWDKA 258

Query: 250 ---FRVYSSDEDSQYLEDHHFDVSDLEPQV--------------------ACPDDVDNVY 286
              ++   SD D+++     FD +D+ P +                    A P+     Y
Sbjct: 259 LAYWKTLYSDADAKFDSVLTFDAADIAPMITYGTNPGMGMGIQEHIPATGAQPEKEKLSY 318

Query: 287 PV----------HRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPAS 336
                         + G  +D  F+GSCTN R EDL+  A+ + D+R  ++V   + P S
Sbjct: 319 QKALDYMGFDDDSSLIGKPVDYVFIGSCTNSRIEDLREVADFVKDKRKADNVTVWIVPGS 378

Query: 337 REIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDP 396
           +++  +A  +G+ + F  AG  +  PGC  CLG +   +  G+  ++T+NRNF GR G  
Sbjct: 379 KQVEQQAKNEGLDKIFEAAGFQLREPGCSACLGMNEDKIPAGKYCVSTSNRNFEGRQGQN 438

Query: 397 ASSVYLANPAVVAESAIEGVIS 418
           A ++ LA+P   A +A+ G I+
Sbjct: 439 ARTL-LASPLTAAAAAVTGKIT 459


Lambda     K      H
   0.320    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 506
Number of extensions: 29
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 428
Length of database: 470
Length adjustment: 33
Effective length of query: 395
Effective length of database: 437
Effective search space:   172615
Effective search space used:   172615
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory