Alignments for a candidate for hom in Pedobacter sp. GW460-11-11-14-LB5

GapMind for Amino acid biosynthesis

Alignments for a candidate for hom in Pedobacter sp. GW460-11-11-14-LB5

Align aspartate kinase; homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (characterized)
to candidate CA265_RS09655 CA265_RS09655 aspartate kinase

Query= reanno::Pedo557:CA265_RS23475
         (817 letters)



>FitnessBrowser__Pedo557:CA265_RS09655
          Length = 437

 Score =  228 bits (580), Expect = 7e-64
 Identities = 162/466 (34%), Positives = 254/466 (54%), Gaps = 38/466 (8%)

Query: 1   MKVLKFGGTSVGSAENIKTLLRLVGEEKQKNSPVVVLSAMSGVTNLLTEMAEMAERGEDY 60
           MK+LKFGGTSVGS E +  LL ++   +++   +VVLSA+SG TN L E+A     G+  
Sbjct: 1   MKILKFGGTSVGSPERMTKLLDIINPNEEQ---IVVLSAVSGTTNSLVEIANYFLAGDKK 57

Query: 61  DTH--LKEIEAKHFAVIRSLLPAAAQNPVFTRLKIFFNELED----LLQAVANLRELSLQ 114
                ++ +  K+   +  LLPAA         +   NE+ D     L  +AN    S++
Sbjct: 58  KGSECVENLYQKYKTFVVELLPAA-------EFQEQGNEVIDYHFGFLAGLANDIFTSIE 110

Query: 115 TKDQILSYGERCSTFMISHIASKNIG-DSIYVNGSDLIKTDSNFGQAKVETELTEMLINN 173
            K  +L+ GE  ST +  HI  K+IG  S+ +   D +KTD +    + +   T   +  
Sbjct: 111 EK-VVLAQGELLSTTLY-HIYLKSIGVPSVLLPALDFMKTDED---NEPDIPFTTKHLTP 165

Query: 174 FYQENKDKVLFVT-GFIASNAAGRVTTLGRGGSDYTAAVWGAALNAEEIEIWTDVNGMMT 232
             +++KD  LF+T G+I  N+ G V  L RGGSDYTA++ GAA+ AEE++IWTD++GM  
Sbjct: 166 LLEQHKDNKLFITQGYICRNSFGEVDNLRRGGSDYTASLLGAAILAEEVQIWTDIDGMHN 225

Query: 233 ADPRMVKKAFSLPELSYTEAMELSYFGAKVIYPPTMIPAFMKKIPIVIKNTFEPDFAGTY 292
            DPR+VK    + +LS+ EA EL+YFGAK+++P ++ PA   KIP+ + NT EP  AGT 
Sbjct: 226 NDPRIVKGTKPIAQLSFDEAAELAYFGAKILHPQSVFPAQKYKIPVRLLNTMEPSAAGTL 285

Query: 293 IKSDVKASSLPIKGISSIDHISIINLTGSGMVGKAGFSGRLFSLLSREQINVVLITQSSS 352
           I  D +     IK I++ D I+ I +  S M+   GF  R+F +  R +  + +IT S  
Sbjct: 286 ITHDSERGK--IKSIAAKDGITAIRIQSSRMLLAYGFLRRVFEVFERYKTPIDMITTSEV 343

Query: 353 EHSITFAVKPTDASQAISLIKKEFELELDAKKLELPEVENNLAVLAIVGENMKRTPGMSG 412
             S+T      D +  +  I +E E           EV+ N +++ +VG+      G + 
Sbjct: 344 AVSLTI-----DETAHLPQIIEELE------SFGTVEVDTNHSIVCVVGDFGSEKHGFAS 392

Query: 413 RLFNALGRNGINVRAIAQGSSEYNISVIISKDDLSKAVNAVHDAFF 458
           R+    G   I +R I+ G S YN+S++I  +  ++A+ ++H+  F
Sbjct: 393 RVLE--GLKHIPIRMISYGGSNYNVSLLILSEYKTEALRSLHNRLF 436


Lambda     K      H
   0.316    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 731
Number of extensions: 42
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 817
Length of database: 437
Length adjustment: 37
Effective length of query: 780
Effective length of database: 400
Effective search space:   312000
Effective search space used:   312000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis