Align Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B; Asp/Glu-ADT subunit B; EC 6.3.5.- (characterized)
to candidate GFF968 PGA1_c09840 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Query= SwissProt::Q9RF06 (475 letters) >FitnessBrowser__Phaeo:GFF968 Length = 504 Score = 397 bits (1021), Expect = e-115 Identities = 211/484 (43%), Positives = 310/484 (64%), Gaps = 16/484 (3%) Query: 3 FETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAYPGVLPVVNKRAVDWAMRA 62 +E VIG+EVH ++ +++K+FS + FGAEPNSN +D A PG+LPV+N V+ A+R Sbjct: 21 WELVIGMEVHAQVSSNAKLFSGASTKFGAEPNSNVAFVDAAMPGMLPVINDYCVEQAVRT 80 Query: 63 AMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGENGYIDIEV-DGETKRIGITRL 121 + L +I S FDRKNYFYPD P+ YQISQ PI G + +E+ DG + + + R+ Sbjct: 81 GLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTARNVRVERI 140 Query: 122 HMEEDAGKSTHKGE--YSLVDLNRQGTPLIEIVSEPDIRSPKEAYAYLEKLRSIIQYTGV 179 HME+DAGKS H + S VDLNR G L+EIVS PDIR P+EA AY+ KLR I+QY G Sbjct: 141 HMEQDAGKSIHDMDPNMSFVDLNRTGVCLMEIVSRPDIRGPEEAAAYIAKLRQIMQYLGT 200 Query: 180 SDVKMEEGSLRCDANISL-RPYGQEKF---------GTKAELKNLNSFNYVRKGLEYEEK 229 D M+ G+LR D N+S+ RP EK+ GT+ E+KN+NS ++++ ++ E + Sbjct: 201 CDGNMQNGNLRADVNVSVCRPGQYEKYQETQDFSHLGTRCEIKNMNSMRFIQQAIDVEAR 260 Query: 230 RQEEELLSGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIVPLYIDDAWKERVR 289 RQ + +GG I QETR +D G+T MR KE + DYRYFP+PD++PL I+ AW + + Sbjct: 261 RQIAIIEAGGTIDQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDDIA 320 Query: 290 QTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHGADVKLTSNWLMGGVN 349 +PELPDE+KA+++ + GL YDA VLT E + FFE T + G KL +NW++ + Sbjct: 321 ANLPELPDEKKARFIKDFGLSDYDASVLTADVESATFFEQTAK-GRSGKLAANWVINELF 379 Query: 350 EYLNKNQ-VELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPELAAKGGNAKQIMEDNG 408 L K++ ++ D+ ++P L G+I LI +S KIAK +F + +GG+ QI+E+ G Sbjct: 380 GRLKKDEDKQITDSPVSPAQLGGIIDLISSDAISGKIAKDLFEIVYTEGGDPAQIVEERG 439 Query: 409 LVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLL 468 + Q++D + ++E + N VE K K G+ VGQ+MKA+ G+ANP+ VN+L+ Sbjct: 440 MKQVTDTGAIEAALDEIIAANPAQVEKAKVNP-KLAGWFVGQVMKATGGKANPKAVNELV 498 Query: 469 KQEL 472 ++L Sbjct: 499 AKKL 502 Lambda K H 0.313 0.133 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 654 Number of extensions: 31 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 475 Length of database: 504 Length adjustment: 34 Effective length of query: 441 Effective length of database: 470 Effective search space: 207270 Effective search space used: 207270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 52 (24.6 bits)
Align candidate GFF968 PGA1_c09840 (aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B)
to HMM TIGR00133 (gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00133.hmm # target sequence database: /tmp/gapView.28736.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00133 [M=481] Accession: TIGR00133 Description: gatB: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-156 507.2 0.0 2.4e-156 506.9 0.0 1.0 1 lcl|FitnessBrowser__Phaeo:GFF968 PGA1_c09840 aspartyl/glutamyl-tR Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Phaeo:GFF968 PGA1_c09840 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 506.9 0.0 2.4e-156 2.4e-156 3 480 .. 20 502 .. 18 503 .. 0.95 Alignments for each domain: == domain 1 score: 506.9 bits; conditional E-value: 2.4e-156 TIGR00133 3 eyelviGlEvHvqlntksKlFckcsneaqeakpNtnvcpvclglPGalPvlNkeavkkAlklalalnskivsevsvF 79 ++elviG+EvH q+ ++KlF+ +s+++ +pN+nv v+ ++PG lPv+N v++A+ +l l++ i+ s F lcl|FitnessBrowser__Phaeo:GFF968 20 DWELVIGMEVHAQVSSNAKLFSGASTKFGA-EPNSNVAFVDAAMPGMLPVINDYCVEQAVRTGLGLKADIN-LWSAF 94 789*************************99.**************************************76.5**** PP TIGR00133 80 dRKhYfYpDlPkgyqitqqdlPiaedGkleieleeke.keigierlhlEeDtgksqykesdkdkqslvDfNRsgvPL 155 dRK+YfYpDlP+gyqi+q + Pi+ +G++ +el ++ +++++er+h+E+D+gks + + +++s+vD+NR+gv L lcl|FitnessBrowser__Phaeo:GFF968 95 DRKNYFYPDLPQGYQISQLYHPIVGEGEVLVELGDGTaRNVRVERIHMEQDAGKSIHDMD--PNMSFVDLNRTGVCL 169 ********************************9765449******************966..79************* PP TIGR00133 156 lEiVtkPdlksakearaflkklrqilryleisdgdleeGsmRvDvNvsirlkGq..........ekegtrvEiKNln 222 +EiV++Pd++ ++ea+a++ klrqi++yl+ dg++++G++R+DvNvs+ Gq ++ gtr EiKN+n lcl|FitnessBrowser__Phaeo:GFF968 170 MEIVSRPDIRGPEEAAAYIAKLRQIMQYLGTCDGNMQNGNLRADVNVSVCRPGQyekyqetqdfSHLGTRCEIKNMN 246 *************************************************98877222222222235679******** PP TIGR00133 223 slksiekaieyEieRqkkllkkgeevkqetrafdekksitvslRkKeeseDYRYfpePdlppieideevvkekveek 299 s++ i++ai+ E Rq+++++ g ++ qetr +d k t s+R+Kee++DYRYfp+Pdl p+ei++ +v++ ++++ lcl|FitnessBrowser__Phaeo:GFF968 247 SMRFIQQAIDVEARRQIAIIEAGGTIDQETRLYDPDKGETRSMRSKEEAHDYRYFPDPDLLPLEIEQAWVDD-IAAN 322 ***********************************************************************9.**** PP TIGR00133 300 lpelPeakrirlkkeyglseedakvlvsdlelldafeevvklikepklavnWileellgeLnk.kkislaeallkpe 375 lpelP++k++r+ k++gls++da vl++d+e + fe+ +k + kla+nW+++el g+L+k ++ +++++ ++p lcl|FitnessBrowser__Phaeo:GFF968 323 LPELPDEKKARFIKDFGLSDYDASVLTADVESATFFEQTAKGRSG-KLAANWVINELFGRLKKdEDKQITDSPVSPA 398 **************************************9998777.***************8735799********* PP TIGR00133 376 elaeliklikegkisqksakelleellenkkdpkklieklgliqisdekelvkiveevikenpkevekyksgkekal 452 +l +i+li+ + is+k+ak+l+e + ++++dp +++e++g+ q++d+ ++ ++++e+i+ np +vek k + k+ lcl|FitnessBrowser__Phaeo:GFF968 399 QLGGIIDLISSDAISGKIAKDLFEIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKVN-PKLA 474 *******************************************************************98755.5788 PP TIGR00133 453 kflvGqvmkktkgradpkevekllkell 480 +++vGqvmk t g+a+pk v++l+ ++l lcl|FitnessBrowser__Phaeo:GFF968 475 GWFVGQVMKATGGKANPKAVNELVAKKL 502 9***********************9987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (481 nodes) Target sequences: 1 (504 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.35 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory