Alignments for a candidate for ilvD in Phaeobacter inhibens BS107

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvD in Phaeobacter inhibens BS107

Align Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 (uncharacterized)
to candidate GFF2757 PGA1_c28000 phosphogluconate dehydratase Edd

Query= curated2:A4YEN4
         (557 letters)



>FitnessBrowser__Phaeo:GFF2757
          Length = 607

 Score =  250 bits (638), Expect = 1e-70
 Identities = 178/532 (33%), Positives = 270/532 (50%), Gaps = 40/532 (7%)

Query: 16  KAPNRAFLKAMG------LTDDDISKPL-------VGVAVAWNEAGPCNIHLLGLSQVVK 62
           K P RA L   G       T +D   PL       +G+  A+N+    +       Q ++
Sbjct: 35  KGPARAHLSCSGQAHAYAATGED-QMPLAEGTAGHLGIVTAYNDMLSAHQPFETYPQRIR 93

Query: 63  EGIRELGGTPRTFTA-PVLIDGIAMGSESMKYSLVSREVIANTVELTVNGHGYDGFVALG 121
           + +R +GGT +     P + DG+  G   M+ SL SR+ IA    + ++ + +D  V LG
Sbjct: 94  DAVRRVGGTAQVAGGVPAMCDGVTQGEAGMELSLFSRDTIAMATGIALSHNVFDATVYLG 153

Query: 122 GCDKTQPGLMMSMARL-NIPSVYMYGGTTLPGNFRGRDIAIGDVYEAVGAFSAGKITAED 180
            CDK  PGL++      ++P+V++  G    G      I+  D  +    F+AG+I  ++
Sbjct: 154 VCDKIVPGLVIGAQVFGHLPAVFLPAGPMTSG------ISNDDKAKVRQKFAAGEIGRDE 207

Query: 181 LRIMEDNAIPGPGACGGLYTANTMAMLSEALGLSLPGSSAPPAVSSDRTKFAKETGRTLM 240
           L   E  A  GPG C    TANT  ML E +GL LPGSS     +  R    +E  R  +
Sbjct: 208 LLKSEMAAYHGPGTCTFYGTANTNQMLMEFMGLHLPGSSFVNPGTDMRAALTEEGARRAL 267

Query: 241 KVMEIG---LKPRDILTFEAFENGIALLMASGGSTNGVLHLLAIAHEAGVSLTLDDFDRI 297
            +  +G       DIL   A+ NGI  LM +GGSTN ++HL+A+A   G+ L   DF  +
Sbjct: 268 ALSALGNDYTPVCDILDERAYVNGIVGLMTTGGSTNLLIHLIAMARAGGIILDWQDFSEL 327

Query: 298 SKKVPEIVNMKPGGDYVMADLYRVGGTPVILKKLLDRGLLHGDTITVTGKTMAQNLSEYK 357
           S  VP +  + P G   +   +  GG   ++ +LL+ G LH DT TV G  +    +E  
Sbjct: 328 SDVVPLLARVYPNGLADVNHFHAAGGLGYVIGQLLNSGHLHSDTRTVAGSGLESYTAEPF 387

Query: 358 I-----------PEFKHDHIVRDLSNPFLPSGGIRILKGSLAPEGSVVKLSASKIKYHRG 406
           +            E  +D I+R  S PF P+GG+  L G+L      +   A + +    
Sbjct: 388 LSDDGLVWKPGAAESLNDKIIRPASAPFQPTGGLARLSGNLGTGVMKISAVAPEHRMVEA 447

Query: 407 PARVFNSEEEAFETVLKKKINEGD-VVVIRYEGPKGGPGMREMLAVTSAI-VGQGLGEKV 464
           PARVF+ +EE        ++++GD ++V+R++GPK   GM E+ ++T  + + QG G+KV
Sbjct: 448 PARVFHDQEEVKAAFKAGELDKGDMIIVVRFQGPKAN-GMPELHSMTPFLGIMQGRGQKV 506

Query: 465 ALVTDGRFSGATRGLMVG-HVAPEAAVGGPIALIRDGDTIVIDGEKGRLDVE 515
           ALVTDGR SGA+  +    HV PEA  GGPI+ +RDGD + +D   G L+V+
Sbjct: 507 ALVTDGRMSGASGKIPSAIHVVPEALDGGPISKLRDGDMVRVDALTGTLEVK 558


Lambda     K      H
   0.316    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 932
Number of extensions: 47
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 557
Length of database: 607
Length adjustment: 36
Effective length of query: 521
Effective length of database: 571
Effective search space:   297491
Effective search space used:   297491
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis