Align Acetate--CoA ligase CCL3; HlCCL3; 2-methylbutanoate--CoA ligase CCL4; 2-methylpropanoate--CoA ligase CCL4; Butanoate--CoA ligase CCL3; Hexanoate--CoA ligase CCL3; Isovalerate--CoA ligase CCL3; Pentanoate--CoA ligase CCL3; Propionate--CoA ligase CCL3; EC 6.2.1.1; EC 6.2.1.-; EC 6.2.1.17 (characterized)
to candidate GFF1184 PGA1_c11990 acyl-CoA synthetase
Query= SwissProt::M4IS88 (568 letters) >FitnessBrowser__Phaeo:GFF1184 Length = 542 Score = 490 bits (1262), Expect = e-143 Identities = 263/555 (47%), Positives = 342/555 (61%), Gaps = 19/555 (3%) Query: 7 DIDDLPKNAANYTALTPLWFLERAATVHPTRTSVIHGSRHYTWLQTYHRCRQFASALNNH 66 D L K AANY LTPL L RAA V +V++G+ T+ Y RC + ASAL Sbjct: 6 DESGLEKTAANYVPLTPLSHLRRAAHVFADVPAVVYGNHRKTYAAYYDRCTRLASALAGM 65 Query: 67 SIGLGSTVAVIAPNVPALYEAHFAVPMAGAVVNCVNIRLNASTIAFLLGHSSAAAVMVDQ 126 + G VA + PN+PA EAHF VP GAV+N +N RL+ ST+A++ H A V+VD Sbjct: 66 GVRPGEVVATLIPNLPAQAEAHFGVPACGAVLNTINTRLDVSTVAYIFDHGEAKVVLVDS 125 Query: 127 EFFSLAEEALKILAQESKSHYKPPLLVVIGDESCDPKTLEYALKTGAIEYEKFLEGGDPE 186 +F +LAEEA + + P ++ V D++ P + + + YE L + Sbjct: 126 QFLTLAEEA-----KAACDGLGPLIIEVPDDQASYPASGRHPI------YEDILAAAAHD 174 Query: 187 FDWKPPEDEWQSISLGYTSGTTASPKGVVLSHRGAYLMSLSASVVWGINEGAIYLWTLPM 246 FDW P+DEW+S++L YTSGTT PKGVV HRGAYLM++ + W + YL +P+ Sbjct: 175 FDWIMPQDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVISWRMVMQPKYLAIVPL 234 Query: 247 FHCNGWCYTWGMAAFCGTNICLRQVTAKGVYSAIAKYGVTHFCAAPVVLNTIVNAPPEEA 306 FHCNGW +TW M GT IC R +TA +Y AIA G THF AP+VLN +VNA E+ Sbjct: 235 FHCNGWNHTWMMPVLGGTLICCRDITAPAIYGAIADEGATHFGGAPIVLNMLVNAAEEDR 294 Query: 307 IIPLPHLVHVMTAGAAPPPSVLFAMSEKGFKVAHTYGLSETYGPSTICAWKPE-WDSLPP 365 H V V TAGA P P+ L + GF V YGL+ETYG T C WK + WD+L Sbjct: 295 R-QFDHTVEVFTAGAPPAPATLEKIEALGFHVTQVYGLTETYGHVTECLWKGDSWDTLDQ 353 Query: 366 IKQARLNARQGVRYIALEGLDVVDTKTMKPVPADGTTMGEIVMRGNAVMKGYLKNPKANE 425 +A + ARQGV + ++ + V+ M+ + +G GEIVMRGN+VMKGYLKNP A Sbjct: 354 QGRAAIKARQGVAFPMMDHITVM-RDDMQQIAMNGQDQGEIVMRGNSVMKGYLKNPDATA 412 Query: 426 ESFADGWFHSGDLAVKHPDGYIEIKDRSKDIIISGGENISSLEVENTLYLHPAVLEVSVV 485 E+F G+FHSGD+AV+HPDGYI+I DR+KDIIISGGENISS+EVE L HP V +VV Sbjct: 413 EAFQGGYFHSGDIAVQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVV 472 Query: 486 ARPDERWGESPCAFVTLKPNIDKSNEQVLAEDIIKFCKSKMPAYWVPKSVVFGPLPKTAT 545 A+PD++WGE PCAFV LKP V D+I+F + + + PK VVF LPKT+T Sbjct: 473 AKPDDKWGEVPCAFVELKP-----GATVDPADLIRFARETLAGFKAPKQVVFQELPKTST 527 Query: 546 GKIQKHVLRAKAKEM 560 GKIQK LR +AK + Sbjct: 528 GKIQKFELRQQAKAL 542 Lambda K H 0.318 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 884 Number of extensions: 27 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 568 Length of database: 542 Length adjustment: 36 Effective length of query: 532 Effective length of database: 506 Effective search space: 269192 Effective search space used: 269192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory