Alignments for a candidate for dapB in Phaeobacter inhibens BS107

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapB in Phaeobacter inhibens BS107

Align 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8) (characterized)
to candidate GFF3239 PGA1_c32920 dihydrodipicolinate reductase DapB

Query= BRENDA::Q2YJN7
         (268 letters)



>FitnessBrowser__Phaeo:GFF3239
          Length = 273

 Score =  306 bits (785), Expect = 2e-88
 Identities = 154/265 (58%), Positives = 194/265 (73%), Gaps = 1/265 (0%)

Query: 2   GLVVVGAGGRMGQTLIRTIQSIEGAKLVGAIERSGSPFLGKDAGEVTGIGTLGVAITDDP 61
           G+V+ GA GRMGQ LI+TI     A LVGA+ER G  ++G+D G   G   +G+ +TDD 
Sbjct: 10  GIVITGASGRMGQMLIKTIADHPRAHLVGAVEREGHDWVGQDVGLAMGGSEIGITVTDDA 69

Query: 62  LPVFAKAHGVLDFTSPAASVEFAGLAAQARIVHVIGTTGCSAEDDEKIRAAARHATIVKS 121
              FAKA  V+DFTSP A++ FAG+AA+A +VHVIGTTG +     ++  A+RH+  +++
Sbjct: 70  PAAFAKAQAVIDFTSPEATIAFAGMAAEAGVVHVIGTTGMNDAQIVQLEPASRHSVQMRA 129

Query: 122 GNMSLGVNLLSVLVQKAAEALGPEDFDIEILEMHHRHKVDAPSGTALLLGEAAARGRDIA 181
           GNMSLGVNLL  L +K A AL  EDFDIE++E HH HKVDAPSGTAL+LGEAAA GR + 
Sbjct: 130 GNMSLGVNLLVQLTKKVAAALD-EDFDIEVIEAHHHHKVDAPSGTALMLGEAAAEGRGVT 188

Query: 182 LADNSVRVRDGYTGPRETGAIGFATLRGGSVIGDHSVILAGTGERVVLSHHAEDRSIFAR 241
           L+D S   RDG TG R+ G IGF+ +RGG ++G+H V+ AG GER+VL H A DR+IFAR
Sbjct: 189 LSDVSDSGRDGITGARKRGDIGFSAIRGGDIVGEHDVLFAGQGERIVLRHLATDRAIFAR 248

Query: 242 GAIKAALWAHGKKPGLYSMLDVLGL 266
           GAIKAALW  GK+PG Y MLDVLGL
Sbjct: 249 GAIKAALWGQGKEPGQYDMLDVLGL 273


Lambda     K      H
   0.318    0.136    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 281
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 268
Length of database: 273
Length adjustment: 25
Effective length of query: 243
Effective length of database: 248
Effective search space:    60264
Effective search space used:    60264
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate GFF3239 PGA1_c32920 (dihydrodipicolinate reductase DapB)
to HMM TIGR00036 (dapB: 4-hydroxy-tetrahydrodipicolinate reductase (EC 1.17.1.8))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00036.hmm
# target sequence database:        /tmp/gapView.28435.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00036  [M=270]
Accession:   TIGR00036
Description: dapB: 4-hydroxy-tetrahydrodipicolinate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
    5.6e-84  267.8   6.0    6.8e-84  267.5   6.0    1.0  1  lcl|FitnessBrowser__Phaeo:GFF3239  PGA1_c32920 dihydrodipicolinate 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__Phaeo:GFF3239  PGA1_c32920 dihydrodipicolinate reductase DapB
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  267.5   6.0   6.8e-84   6.8e-84       4     270 .]      11     272 ..       9     272 .. 0.97

  Alignments for each domain:
  == domain 1  score: 267.5 bits;  conditional E-value: 6.8e-84
                          TIGR00036   4 vavaGaaGrmGrevikavkeaedlelvaalerkgsskqgkDiGelagigkvgvpveddleavkvlaekkadvliDf 79 
                                        ++++Ga+GrmG+ +ik++++++  +lv+a+er+g++ +g+D+G  +g +++g++v+dd  a+      ka+ +iDf
  lcl|FitnessBrowser__Phaeo:GFF3239  11 IVITGASGRMGQMLIKTIADHPRAHLVGAVEREGHDWVGQDVGLAMGGSEIGITVTDDAPAA----FAKAQAVIDF 82 
                                        89********************************************************9987....778899**** PP

                          TIGR00036  80 ttpeavlenvkialekgvrlVvGTTGfseedlkelkdlaekkgvalviapNfaiGvnlllkllekaakvle.dvDi 154
                                        t pea+   + +a+e+gv  V+GTTG++++++ +l+ ++ + +v  + a N+++Gvnll++l++k+a  l+ d+Di
  lcl|FitnessBrowser__Phaeo:GFF3239  83 TSPEATIAFAGMAAEAGVVHVIGTTGMNDAQIVQLEPASRH-SV-QMRAGNMSLGVNLLVQLTKKVAAALDeDFDI 156
                                        *************************************9999.55.569*****************999997256** PP

                          TIGR00036 155 EiiElHHrhKkDaPSGTAlklaeiiakargkdlkeaaveeregltGerkkeeiGiaavRggdvvgehtvlFasdGe 230
                                        E+iE+HH+hK+DaPSGTAl l+e+ a+ rg +l+++  ++r+g+tG+rk+ +iG++a+Rggd+vgeh vlFa++Ge
  lcl|FitnessBrowser__Phaeo:GFF3239 157 EVIEAHHHHKVDAPSGTALMLGEAAAEGRGVTLSDVSDSGRDGITGARKRGDIGFSAIRGGDIVGEHDVLFAGQGE 232
                                        **************************************************************************** PP

                          TIGR00036 231 rleitHkassRaafakGvvrairwledkeekvydledvld 270
                                        r+ ++H a++Ra+fa+G+++a+ w + ke + yd+ dvl+
  lcl|FitnessBrowser__Phaeo:GFF3239 233 RIVLRHLATDRAIFARGAIKAALWGQGKEPGQYDMLDVLG 272
                                        **************************************96 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (273 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 7.90
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis