Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate GFF1906 PGA1_c19360 acetolactate synthase isozyme, large subunit
Query= curated2:O08353 (599 letters) >FitnessBrowser__Phaeo:GFF1906 Length = 601 Score = 302 bits (773), Expect = 3e-86 Identities = 183/569 (32%), Positives = 290/569 (50%), Gaps = 38/569 (6%) Query: 4 AEAMIKALEAEKVEILFGYPGGALLPFYDALHHSDLIHLLTRHEQAAAHAADGYARASGK 63 AE ++ L VE +FG G + AL S + + RHEQ A+HAAD YAR +G+ Sbjct: 16 AEHIVDFLGRRNVEHVFGLCGHTNIAVLAALADSPIDFITVRHEQIASHAADAYARVTGR 75 Query: 64 VGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALG------L 117 V + PG TN TGVA A D PMV + G +PT G QE++ + Sbjct: 76 ASVVLSHLSPGLTNCATGVANAALDCVPMVVIAGDIPTHYYGKHPHQEVNLHADAAQWEI 135 Query: 118 FMPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKV 177 + P VK +++ + + EI AF +A++G+PGPV +++P D+ + D Sbjct: 136 YRPFVKRAWRVDRADLMAEILEKAFHLAESGQPGPVLVNVPMDIFSEVISSDTFD----- 190 Query: 178 KLIGYNPTTIGHPRQ----IKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIP 233 I N T+ P ++ + +A+AK P+ GGG+LL+ A+ E+ + + +P Sbjct: 191 -RIASNTKTLVKPSMDDETARRIVSGLAAAKDPVAYIGGGILLAQASAEIEEFATHMGLP 249 Query: 234 VCTTLMGKGCISENHPLALGMVGMHGTKPANYCLSESDVLISIGCRFSDR--------IT 285 + +LMGKG + ++HPL +GM G GT+ N +DV+ ++G RF + T Sbjct: 250 IAHSLMGKGAVRDDHPLVMGMTGFWGTELVNQTCLNADVVFAVGTRFKEADCSSWYPGYT 309 Query: 286 GDIKSFATNAKIIHIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENND 345 +I + + K+IHIDI+P EIG+N ++ +V DAK L+ + + + Sbjct: 310 FNIGAKGNDTKVIHIDIEPQEIGRNYPTEIGVVADAKAALRVLTRVAKDMYPDGFNRTEK 369 Query: 346 KENISQWIENVNSLKKSSIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQN 405 K I+ + E+ K S++ + P+ P++I+ + + D + IITTDVG N Sbjct: 370 KAEIAAFRED---FKASNVEMQTSAAFPMMPERILADTRIALPD-----DAIITTDVGWN 421 Query: 406 QMWMAHYFKTQTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQEL 465 + + F TP S L+ GG TMGFG P+AIGAK+A P+ V+ + GDGGF N L Sbjct: 422 KNGVGQQFDILTPGSILTPGGFATMGFGPPAAIGAKLAAPERVVLSLVGDGGFGQNPSML 481 Query: 466 GTIAEYNIPVVICIFDNRTLGMVYQWQNLFYGKRQCSVNFGGA------PDFIKLAESYG 519 T E N+ ++ + +N G + Q YG + G A P + ++A +YG Sbjct: 482 ATAVELNLGIIWLVMNNNAFGTIAGLQKAHYGLTYGTTFPGSAAAPTNGPGYAEIARAYG 541 Query: 520 IKARRIESPNEINEALKEAINCDEPYLLD 548 + RI S +E+ AL+ AI +P +LD Sbjct: 542 AEGIRISSADELLPALQAAIASGKPTVLD 570 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 844 Number of extensions: 42 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 599 Length of database: 601 Length adjustment: 37 Effective length of query: 562 Effective length of database: 564 Effective search space: 316968 Effective search space used: 316968 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory