Align periplasmic dehydroquinate dehydratase (EC 4.2.1.10) (characterized)
to candidate PP_2407 PP_2407 3-dehydroquinate dehydratase 2, type II
Query= metacyc::MONOMER-15328 (160 letters) >FitnessBrowser__Putida:PP_2407 Length = 149 Score = 182 bits (463), Expect = 2e-51 Identities = 85/145 (58%), Positives = 107/145 (73%) Query: 14 RPLITVLNGPNLNMLGLRQPGIYGHATLDDVEQVCIQAAERLDVAIDFRQTNGEGELVSW 73 +PLI VLNGPNLNMLG R+P YGH TL D+ Q C A + I+FRQTN EGEL+ W Sbjct: 2 KPLILVLNGPNLNMLGTREPAQYGHETLADLAQGCADTAHAHGLEIEFRQTNHEGELIDW 61 Query: 74 VQECRGRADGIVINPAAYGHTSIALLDALLAVELPVIEVHISNIHRREPFRHHTYVSQAA 133 + RGR GIVINP A+ HTS+A+ DAL+A ELPVIEVH+SN+H+REPFRH ++VS A Sbjct: 62 IHAARGRCAGIVINPGAWTHTSVAIRDALVASELPVIEVHLSNVHKREPFRHLSFVSSIA 121 Query: 134 IGVICGLGVRGYAHALQAITDMIED 158 +GVICGLG GY AL +++++ Sbjct: 122 VGVICGLGSHGYRMALSHFAELLQE 146 Lambda K H 0.322 0.139 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 124 Number of extensions: 2 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 160 Length of database: 149 Length adjustment: 17 Effective length of query: 143 Effective length of database: 132 Effective search space: 18876 Effective search space used: 18876 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 43 (21.2 bits)
Align candidate PP_2407 PP_2407 (3-dehydroquinate dehydratase 2, type II)
to HMM TIGR01088 (aroQ: 3-dehydroquinate dehydratase, type II (EC 4.2.1.10))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01088.hmm # target sequence database: /tmp/gapView.30283.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01088 [M=141] Accession: TIGR01088 Description: aroQ: 3-dehydroquinate dehydratase, type II Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.3e-64 200.2 0.0 6e-64 200.0 0.0 1.0 1 lcl|FitnessBrowser__Putida:PP_2407 PP_2407 3-dehydroquinate dehydra Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Putida:PP_2407 PP_2407 3-dehydroquinate dehydratase 2, type II # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 200.0 0.0 6e-64 6e-64 2 139 .. 5 142 .. 4 144 .. 0.98 Alignments for each domain: == domain 1 score: 200.0 bits; conditional E-value: 6e-64 TIGR01088 2 ilvlnGPnlnlLGkrepkvyGsltleeieelleeaakelevevevfqsnsegelidkihealeqvdgivinpaal 76 ilvlnGPnln+LG+rep+ yG+ tl ++ + + ++a+++++e+e++q+n+egelid+ih a ++ givinp+a+ lcl|FitnessBrowser__Putida:PP_2407 5 ILVLNGPNLNMLGTREPAQYGHETLADLAQGCADTAHAHGLEIEFRQTNHEGELIDWIHAARGRCAGIVINPGAW 79 89************************************************************************* PP TIGR01088 77 thtsvalrDalaavslPvvevhlsnvhareefrkksvlaevakGvivGlGakgyklalealve 139 thtsva+rDal+a +lPv+evhlsnvh+re+fr+ s+++ +a Gvi+GlG +gy++al+ +e lcl|FitnessBrowser__Putida:PP_2407 80 THTSVAIRDALVASELPVIEVHLSNVHKREPFRHLSFVSSIAVGVICGLGSHGYRMALSHFAE 142 **********************************************************98876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (141 nodes) Target sequences: 1 (149 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 4.62 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory