Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate PP_0931 PP_0931 Glutamyl-tRNA(Gln) amidotransferase subunit A
Query= SwissProt::O06491 (485 letters) >FitnessBrowser__Putida:PP_0931 Length = 483 Score = 494 bits (1272), Expect = e-144 Identities = 245/480 (51%), Positives = 335/480 (69%), Gaps = 3/480 (0%) Query: 6 HKIT--ELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDEAVD 63 H++T E+ + + K +L RI+ +D ++ +F+++ E+ A A+ D A Sbjct: 2 HQLTLAEIARGLADKSFSSEELTGALLARIKQLDPQINSFISVTEDLALGQARAAD-ARR 60 Query: 64 GRSEHGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIGKLN 123 E L G PI KD T G+RT+C SK+L+NF YDATVV +L +A VT+GK N Sbjct: 61 AAGETSALLGAPIAHKDLFCTNGVRTSCGSKMLDNFKAPYDATVVAKLAEAGMVTLGKTN 120 Query: 124 MDEFAMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIRQPA 183 MDEFAMGS+ E+S Y KNPWNL+ VPGGSSGGSAAAVAA +P + G+DTGGSIRQPA Sbjct: 121 MDEFAMGSANESSHYGAVKNPWNLEHVPGGSSGGSAAAVAARLLPATTGTDTGGSIRQPA 180 Query: 184 SFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTSANV 243 + + GLKPTYGRVSR+G++A+ASSLDQ GP+ RT ED A LLQ ++G D DSTS Sbjct: 181 ALTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCALLLQGMAGFDAKDSTSIEE 240 Query: 244 DVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLPHSK 303 VPD+ +SL ++GL+I +PKEY G G+ + V A++K LE LGA +E+SLP+ + Sbjct: 241 PVPDYSASLNASLQGLRIGLPKEYFGAGLDPRIADLVQASVKELEKLGAVVKEISLPNMQ 300 Query: 304 YALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIMLGT 363 +A+ YY+++ +EAS+NL+RFDG+R+GYR + +L DLYK++R EGFG EV+RRIM+GT Sbjct: 301 HAIPAYYVIAPAEASSNLSRFDGVRFGYRCEEPKDLTDLYKRSRGEGFGVEVQRRIMVGT 360 Query: 364 FALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLTMYA 423 +ALS+GYYDAYY KAQ++R LIK DF F D+I+GPTTP PA+K+G + DP+ Y Sbjct: 361 YALSAGYYDAYYVKAQQIRRLIKNDFMAAFNDVDLILGPTTPNPAWKLGAKSSDPVAAYL 420 Query: 424 NDILTIPVNLAGVPGISVPCGLADGLPLGLQIIGKHFDESTVYRVAHAFEQATDHHKAKP 483 D+ TI NLAG+PG+S+P G DGLP+G+Q++ +F E + VAH ++Q TD H P Sbjct: 421 EDVYTITANLAGLPGLSMPAGFVDGLPVGVQLLAPYFQEGRLLNVAHRYQQVTDWHTRAP 480 Lambda K H 0.315 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 558 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 483 Length adjustment: 34 Effective length of query: 451 Effective length of database: 449 Effective search space: 202499 Effective search space used: 202499 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 52 (24.6 bits)
Align candidate PP_0931 PP_0931 (Glutamyl-tRNA(Gln) amidotransferase subunit A)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00132.hmm # target sequence database: /tmp/gapView.24142.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00132 [M=466] Accession: TIGR00132 Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.9e-180 585.5 0.0 4.4e-180 585.3 0.0 1.0 1 lcl|FitnessBrowser__Putida:PP_0931 PP_0931 Glutamyl-tRNA(Gln) amido Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Putida:PP_0931 PP_0931 Glutamyl-tRNA(Gln) amidotransferase subunit A # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 585.3 0.0 4.4e-180 4.4e-180 5 465 .. 13 474 .. 9 475 .. 0.98 Alignments for each domain: == domain 1 score: 585.3 bits; conditional E-value: 4.4e-180 TIGR00132 5 lkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdniavkdiett 77 l +k++s++e++ ++l+ri++ + +in+f++vt++ al +a++ d++ a e + l g pia Kd +++++++t+ lcl|FitnessBrowser__Putida:PP_0931 13 LADKSFSSEELTGALLARIKQLDPQINSFISVTEDLALGQARAADARRAaGEtSALLGAPIAHKDLFCTNGVRTS 87 678899999************************************998876647********************* PP TIGR00132 78 caSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervpGGSsgGsaaavaad 152 c+Sk+L+n+++pydatVv +l eag++++GktN+DEFamGs+ e+S +g++knP+n e+vpGGSsgGsaaavaa lcl|FitnessBrowser__Putida:PP_0931 88 CGSKMLDNFKAPYDATVVAKLAEAGMVTLGKTNMDEFAMGSANESSHYGAVKNPWNLEHVPGGSSGGSAAAVAAR 162 *************************************************************************** PP TIGR00132 153 lvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkvedialvldvisgkDkkDsts 227 l p + g+DTGgSiRqPA++++ GlKPtYG+vSR+G++ayasSldq G+la++ ed al+l+ ++g D+kDsts lcl|FitnessBrowser__Putida:PP_0931 163 LLPATTGTDTGGSIRQPAALTNLTGLKPTYGRVSRWGMIAYASSLDQGGPLARTAEDCALLLQGMAGFDAKDSTS 237 *************************************************************************** PP TIGR00132 228 levkveelleelkkdlkglkvgvvkelseesldkevkekfekllekleelgaeivevslpsvklalaiYyiisps 302 +e +v+++ +l++ l+gl++g+ ke+++ +ld ++++ +++ +++le+lga ++e+slp+++ a+++Yy+i+p+ lcl|FitnessBrowser__Putida:PP_0931 238 IEEPVPDYSASLNASLQGLRIGLPKEYFGAGLDPRIADLVQASVKELEKLGAVVKEISLPNMQHAIPAYYVIAPA 312 *************************************************************************** PP TIGR00132 303 EassnlarydgiryGkrveelkslkelyaktRsegfgeevkrRimlGayalskeyydkyykkAqkvrtliidefe 377 Eassnl+r+dg+r+G+r ee+k+l++ly+++R egfg ev+rRim+G+yals++yyd+yy kAq++r+li+++f lcl|FitnessBrowser__Putida:PP_0931 313 EASSNLSRFDGVRFGYRCEEPKDLTDLYKRSRGEGFGVEVQRRIMVGTYALSAGYYDAYYVKAQQIRRLIKNDFM 387 *************************************************************************** PP TIGR00132 378 klfeevDvivsptaptlafklgekaedplemylsDvltvpanlaGlpaisvPlgkkekglpiGlqiigkafddkk 452 +f++vD+i++pt+p a klg+k +dp++ yl+Dv+t++anlaGlp++s+P+g +glp+G+q+ + +f++ + lcl|FitnessBrowser__Putida:PP_0931 388 AAFNDVDLILGPTTPNPAWKLGAKSSDPVAAYLEDVYTITANLAGLPGLSMPAGFV-DGLPVGVQLLAPYFQEGR 461 ********************************************************.7***************** PP TIGR00132 453 llsvakaleqald 465 ll+va+ ++q +d lcl|FitnessBrowser__Putida:PP_0931 462 LLNVAHRYQQVTD 474 ********99876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (466 nodes) Target sequences: 1 (483 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 10.37 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory