Alignments for a candidate for glnA in Pseudomonas putida KT2440

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Pseudomonas putida KT2440

Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate PP_4399 PP_4399 putative Glutamine synthetase

Query= BRENDA::O33342
         (457 letters)



>FitnessBrowser__Putida:PP_4399
          Length = 413

 Score =  186 bits (471), Expect = 2e-51
 Identities = 129/377 (34%), Positives = 190/377 (50%), Gaps = 19/377 (5%)

Query: 85  GDMVMTPDLSTLRLIPWLP-GTALVIADLVWADGSEVAVSPRSILRRQLDRLKARGLVAD 143
           GD+ +  + S +  +PW   G AL I D    DG   A+S R  L+  + R  A GL   
Sbjct: 38  GDLALVAEPSQVHRLPWSDDGRALAICDANELDGRPSALSTRGQLKAVIARYAALGLAPV 97

Query: 144 VATELEFIVF------DQPYRQAWAS-GYRGLTPASDYNIDYAILASSRMEPLLRDIRLG 196
           VATELEF VF       QP+     S G R L  ++     +++ +++ + P  +++   
Sbjct: 98  VATELEFFVFAPNTDPQQPFLPPVGSDGRRELGHSA-----FSVSSNNGLRPFFKEVYHC 152

Query: 197 MAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGKSLTFMAKY 256
           MA  GL  +    E  + Q EI   + + L+  D   ++K+  KE+A +HG ++  MAK 
Sbjct: 153 MAALGLPRDTFMHEMGVSQFEINLLHGDPLLLADQTFLFKHLLKEVALKHGLTVVCMAKP 212

Query: 257 DERE-GNSCHIHVSL-RGTDGSAVFADSNGPHGMSSMFRSFVAGQLATLREFTLCYAPTI 314
             R  G+S HIH SL     G  VF+D  G    +  F  F+ G  A + +FT  +AP +
Sbjct: 213 LARTPGSSMHIHQSLVEAGSGRNVFSDEQGLP--TETFHHFIGGLQACMADFTALFAPNV 270

Query: 315 NSYKRFADSSFAPTALAWGLDNRTCALRVVGHGQNI-RVECRVPGGDVNQYLAVAALIAG 373
           NSY+R      +P    W  DNR   LR+        RVE R+PG D N YLA+AA +A 
Sbjct: 271 NSYQRLCHPYASPNNACWSEDNRAAGLRIPASAPVARRVENRLPGADANPYLAIAASLAA 330

Query: 374 GLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGEDVVAHYLNN 433
           GL+GIE+ LQ P P +   ++  +   LP TL  A    + S L RE FG + +  Y+ +
Sbjct: 331 GLHGIEQRLQ-PSPAIQGEFEVPEHLSLPCTLHAALERLKRSTLARELFGSEFIEGYIAS 389

Query: 434 ARVELAAFNAAVTDWER 450
             +EL+ F   +T WER
Sbjct: 390 KTLELSDFFDEITPWER 406


Lambda     K      H
   0.321    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 497
Number of extensions: 38
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 413
Length adjustment: 32
Effective length of query: 425
Effective length of database: 381
Effective search space:   161925
Effective search space used:   161925
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis