Align Homoserine kinase; HK; HSK; EC 2.7.1.39 (characterized)
to candidate PP_0121 PP_0121 homoserine kinase
Query= SwissProt::P29364 (316 letters) >FitnessBrowser__Putida:PP_0121 Length = 316 Score = 457 bits (1175), Expect = e-133 Identities = 223/316 (70%), Positives = 257/316 (81%) Query: 1 MSVFTPLERSTLEAFLAPYDLGRLRDFRGIAEGSENSNFFVSLEHGEFVLTLVERGPVQD 60 MSVFTP+ R LE FLAPY+LGRL DF+GIA G+ENSNFFVSLE GEFVLTL+ERGP +D Sbjct: 1 MSVFTPVTRPELETFLAPYELGRLLDFQGIAAGTENSNFFVSLEQGEFVLTLIERGPSED 60 Query: 61 LPFFIELLDVLHEDGLPVPYALRTRDGEALRRLEGKPALLQPRLAGRHERQPNAHHCQEV 120 +PFFIELLD LH +PVPYA+R RDG LR L GKPALLQPRL+G+H + PN HC +V Sbjct: 61 MPFFIELLDTLHGADMPVPYAIRDRDGNGLRELCGKPALLQPRLSGKHIKAPNNQHCAQV 120 Query: 121 GDLLGHLHAATRGRILERPSDRGLPWMLEQGANLAPRLPEQARALLAPALAEIAALDAER 180 G+LL H+H ATR I+ER +DRGL WML GA L PRL + ALL PAL EI+A A+ Sbjct: 121 GELLAHIHLATREHIIERRTDRGLDWMLASGAELLPRLTAEQAALLQPALDEISAHKAQI 180 Query: 181 PALPRANLHADLFRDNVLFDGPHLAGLIDFYNACSGWMLYDLAITLNDWCSNTDGSLDPA 240 ALPRANLHADLFRDNV+F+G HL G+IDFYNACSG MLYD+AIT+NDWC + G++D Sbjct: 181 LALPRANLHADLFRDNVMFEGTHLTGVIDFYNACSGPMLYDIAITVNDWCLDEQGAVDVP 240 Query: 241 RARALLAAYANRRPFTALEAEHWPSMLRVACVRFWLSRLIAAEAFAGQDVLIHDPAEFEI 300 RA+ALLAAYA RPFTA EAE WP MLRV CVRFWLSRLIAAE+FAG DV+IHDP+EFE+ Sbjct: 241 RAQALLAAYAALRPFTAAEAELWPEMLRVGCVRFWLSRLIAAESFAGMDVMIHDPSEFEV 300 Query: 301 RLAQRQNVEIHLPFAL 316 RLAQRQ V +HLPFAL Sbjct: 301 RLAQRQQVALHLPFAL 316 Lambda K H 0.324 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 382 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 316 Length of database: 316 Length adjustment: 27 Effective length of query: 289 Effective length of database: 289 Effective search space: 83521 Effective search space used: 83521 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 48 (23.1 bits)
Align candidate PP_0121 PP_0121 (homoserine kinase)
to HMM TIGR00938 (thrB: homoserine kinase (EC 2.7.1.39))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00938.hmm # target sequence database: /tmp/gapView.18909.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00938 [M=307] Accession: TIGR00938 Description: thrB_alt: homoserine kinase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.3e-93 297.0 0.0 9.5e-93 296.8 0.0 1.0 1 lcl|FitnessBrowser__Putida:PP_0121 PP_0121 homoserine kinase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Putida:PP_0121 PP_0121 homoserine kinase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 296.8 0.0 9.5e-93 9.5e-93 1 306 [. 1 303 [. 1 304 [. 0.96 Alignments for each domain: == domain 1 score: 296.8 bits; conditional E-value: 9.5e-93 TIGR00938 1 mavytsvsdeeleafLegydlGellslkGiaeGvensnyllttdkgryvLtlyekrvkaeeLPfflellthLaer 75 m+v+t v+ ele+fL y+lG+ll+++Gia G ensn+++ ++g +vLtl e+ +e++Pff+ell+ L lcl|FitnessBrowser__Putida:PP_0121 1 MSVFTPVTRPELETFLAPYELGRLLDFQGIAAGTENSNFFVSLEQGEFVLTLIERGP-SEDMPFFIELLDTLHGA 74 9*****************************************************999.9**************** PP TIGR00938 76 glpvakpvksrdGralseLaGkPaalvefLkGssvakPtaercrevgevlaklhlagadfkeerkndlrleaWsi 150 ++pv+ +++ rdG+ l eL GkPa l L+G+ ++ P ++c++vge la++hla+ ++ er+ r +W lcl|FitnessBrowser__Putida:PP_0121 75 DMPVPYAIRDRDGNGLRELCGKPALLQPRLSGKHIKAPNNQHCAQVGELLAHIHLATREHIIERRT-DRGLDWML 148 *********************************************************887777777.48889*99 PP TIGR00938 151 laakkfkvleqleeelaalldkeldalkkflpr..dLPrgvihadlfkdnvlldgdklkgvidfyfaCedallyd 223 + ++ +l+ l +e+aall+ ld ++ ++ LPr+ +hadlf+dnv+++g +l+gvidfy aC++ +lyd lcl|FitnessBrowser__Putida:PP_0121 149 ASGAE--LLPRLTAEQAALLQPALDEISAHKAQilALPRANLHADLFRDNVMFEGTHLTGVIDFYNACSGPMLYD 221 88888..******************999887763379************************************** PP TIGR00938 224 laiavndWcfeaddkldaaaakallkgyeavrpLseeekaafpvllrgaalrfllsrlldlvftqagelvvakdP 298 +ai+vndWc+++++ +d+ +a+all +y a rp+++ e + +p +lr++++rf+lsrl + ag v++ dP lcl|FitnessBrowser__Putida:PP_0121 222 IAITVNDWCLDEQGAVDVPRAQALLAAYAALRPFTAAEAELWPEMLRVGCVRFWLSRLIAAE-SFAGMDVMIHDP 295 **********************************************************9765.57899999**** PP TIGR00938 299 aeferkLk 306 efe +L+ lcl|FitnessBrowser__Putida:PP_0121 296 SEFEVRLA 303 ****9986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (307 nodes) Target sequences: 1 (316 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 10.46 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory