Align ketol-acid reductoisomerase [NAD(P)+] (EC 1.1.1.383) (characterized)
to candidate 6939188 Sama_3282 ketol-acid reductoisomerase (RefSeq)
Query= BRENDA::A0KS29 (492 letters) >FitnessBrowser__SB2B:6939188 Length = 493 Score = 885 bits (2286), Expect = 0.0 Identities = 439/492 (89%), Positives = 462/492 (93%) Query: 1 MANYFNSLNLRQQLAQLGQCRFMDRSEFSDGCNYIKDWNIVILGCGAQGLNQGLNMRDSG 60 MANYFNSLNLRQQLAQL QCRFMDRSEF +GC+YIK WNIVILGCGAQGLNQGLNMRDSG Sbjct: 1 MANYFNSLNLRQQLAQLSQCRFMDRSEFENGCDYIKGWNIVILGCGAQGLNQGLNMRDSG 60 Query: 61 LNIAYALRPEAIAQKRASWQKATDNGFKVGTFEELIPTADLVLNLTPDKQHSNVVSAVMP 120 LNI+YALR EAIA+KRASW+KATDNGF VGTFEELIP ADLVLNLTPDKQHSNVV+ VMP Sbjct: 61 LNISYALRAEAIAEKRASWKKATDNGFTVGTFEELIPQADLVLNLTPDKQHSNVVNTVMP 120 Query: 121 LMKQGATLSYSHGFNIVEEGMQIRPDITVVMVAPKCPGTEVREEYKRGFGVPTLIAVHPE 180 LMKQGATLSYSHGFNIVEEGMQ+RPDITV+MVAPKCPGTEVREEYKRGFGVPTLIAVHPE Sbjct: 121 LMKQGATLSYSHGFNIVEEGMQVRPDITVIMVAPKCPGTEVREEYKRGFGVPTLIAVHPE 180 Query: 181 NDPNGDGLEIAKAYASATGGDRAGVLQSSFIAEVKSDLMGEQTILCGMLQTGAILGYDKM 240 NDP GDGLEIAKAYASATGGDRAGVL SSFIAEVKSDLMGEQTILCGMLQT AILGY+KM Sbjct: 181 NDPRGDGLEIAKAYASATGGDRAGVLHSSFIAEVKSDLMGEQTILCGMLQTSAILGYEKM 240 Query: 241 VADGVEPGYAAKLIQQGWETVTEALKHGGITNMMDRLSNPAKIKAFEIAEDLKEILQPLF 300 VADGVEPGYAAKLIQQG ET+TEALK GGITNMMDRLSNPAKIKAF++AE+LK IL PLF Sbjct: 241 VADGVEPGYAAKLIQQGLETITEALKQGGITNMMDRLSNPAKIKAFDMAEELKGILAPLF 300 Query: 301 EKHMDDIISGEFSRTMMQDWANDDANLLRWRAETAETGFENAPVSSEHIDEQTYFDKGIF 360 EKHMDDIISGEFSRTMM DWANDDANLLRWRAET ET FENAP S+EHIDEQTYFDKGIF Sbjct: 301 EKHMDDIISGEFSRTMMADWANDDANLLRWRAETNETAFENAPSSNEHIDEQTYFDKGIF 360 Query: 361 LVAMIKAGVELAFDTMVSAGIVEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYG 420 LVAMIKAGVELAFDTMV AGI+EESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYG Sbjct: 361 LVAMIKAGVELAFDTMVDAGIIEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYG 420 Query: 421 CYLFNHAAVPMLRDYVNAMSPEYLGAGLKDSSNNVDNLQLIAINDAIRHTSVEYIGAELR 480 CYLFNHAAVP+LRDYV +MS EYLG GLKDSSN VDN++LI +N AIR+T+VE+IG ELR Sbjct: 421 CYLFNHAAVPLLRDYVKSMSAEYLGGGLKDSSNAVDNVRLIEVNQAIRNTAVEFIGEELR 480 Query: 481 GYMTDMKSIVGA 492 GYM DMK I A Sbjct: 481 GYMKDMKRISAA 492 Lambda K H 0.317 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 903 Number of extensions: 17 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 492 Length of database: 493 Length adjustment: 34 Effective length of query: 458 Effective length of database: 459 Effective search space: 210222 Effective search space used: 210222 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate 6939188 Sama_3282 (ketol-acid reductoisomerase (RefSeq))
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00465.hmm # target sequence database: /tmp/gapView.23456.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00465 [M=314] Accession: TIGR00465 Description: ilvC: ketol-acid reductoisomerase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-135 437.5 0.2 2.5e-124 400.3 0.0 2.0 2 lcl|FitnessBrowser__SB2B:6939188 Sama_3282 ketol-acid reductoisom Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6939188 Sama_3282 ketol-acid reductoisomerase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 400.3 0.0 2.5e-124 2.5e-124 2 312 .. 36 367 .. 35 368 .. 0.98 2 ! 40.9 0.0 6.3e-15 6.3e-15 185 311 .. 361 484 .. 359 487 .. 0.86 Alignments for each domain: == domain 1 score: 400.3 bits; conditional E-value: 2.5e-124 TIGR00465 2 kgkkvaiiGyGsqGeaqalnlrdsglnvivglrkea.....aswkkAeedGfkvltveeaikkadlimiLlpDevqk 73 kg +++i+G+G+qG +q+ln+rdsgln++++lr ea aswkkA+++Gf v t+ee+i++adl+++L+pD+ q+ lcl|FitnessBrowser__SB2B:6939188 36 KGWNIVILGCGAQGLNQGLNMRDSGLNISYALRAEAiaekrASWKKATDNGFTVGTFEELIPQADLVLNLTPDK-QH 111 7789*******************************999*99*********************************.88 PP TIGR00465 74 evyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsliAveqd..vtgeak 148 ++++++++pl+k+g++l++sHGfniv+++++++ d++v++vAPK+pG++vReeyk+g+Gvp+liAv+++ + g++ lcl|FitnessBrowser__SB2B:6939188 112 SNVVNTVMPLMKQGATLSYSHGFNIVEEGMQVRPDITVIMVAPKCPGTEVREEYKRGFGVPTLIAVHPEndPRGDGL 188 889999************************************************************988779***** PP TIGR00465 149 eiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyqpelAyfeivhelklivdll 225 eiA+ayA a+Gg ragvl ++F +Ev+sDL+GEq++LcG+l+ ++++++v++G +p +A ++i+++l++i+++l lcl|FitnessBrowser__SB2B:6939188 189 EIAKAYASATGGDRAGVLHSSFIAEVKSDLMGEQTILCGMLQTSAILGYEKMVADGVEPGYAAKLIQQGLETITEAL 265 ***************************************************************************** PP TIGR00465 226 kekGlelmrdavsntAklgalelreilkeelkkemqkilkeiqnGefak....ewalek........eagkpafeea 290 k++G+++m+d++sn+Ak++a++++e lk l++ ++k++++i++Gef++ +wa+++ e++++afe+a lcl|FitnessBrowser__SB2B:6939188 266 KQGGITNMMDRLSNPAKIKAFDMAEELKGILAPLFEKHMDDIISGEFSRtmmaDWANDDanllrwraETNETAFENA 342 *************************************************9999*****9****************** PP TIGR00465 291 rkke...keqeiekvGkelralvka 312 + ++ eq ++++G l a++ka lcl|FitnessBrowser__SB2B:6939188 343 PSSNehiDEQTYFDKGIFLVAMIKA 367 99888999**************986 PP == domain 2 score: 40.9 bits; conditional E-value: 6.3e-15 TIGR00465 185 LcGglealikaafdtLveaGyqpelAyfeivhelklivdllkekGlelmrdavsntAklgalelreilkeelkkemq 261 L+ +++a++++afdt+v+aG +e Ay e +he li++ + +k l +m +s+tA++g + ++ l+ ++ lcl|FitnessBrowser__SB2B:6939188 361 LVAMIKAGVELAFDTMVDAGIIEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYGCYLFNHAAVPLLRDYVK 437 889**********************************************************9988888888887777 PP TIGR00465 262 kilkeiqnGefakewalekeagkpafeearkkekeqeiekvGkelralvk 311 + e G +++++ ++ e +++ +++ +e +G+elr +k lcl|FitnessBrowser__SB2B:6939188 438 SMSAEYLGGGLKD---SSNAVDNVRLIEVNQAIRNTAVEFIGEELRGYMK 484 7777766666544...3445678899999999999999999999998876 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (314 nodes) Target sequences: 1 (493 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.04u 0.01s 00:00:00.05 Elapsed: 00:00:00.05 # Mc/sec: 2.97 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory