Align Acetolactate synthase isozyme 2 large subunit; AHAS-II; ALS-II; Acetohydroxy-acid synthase II large subunit; EC 2.2.1.6 (characterized)
to candidate 6939187 Sama_3281 acetolactate synthase 2 catalytic subunit (RefSeq)
Query= SwissProt::P0DP90 (548 letters) >FitnessBrowser__SB2B:6939187 Length = 556 Score = 729 bits (1883), Expect = 0.0 Identities = 358/547 (65%), Positives = 434/547 (79%) Query: 1 MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLCRHEQGAAMAAIGYARA 60 M GA V+ L A GVNTVFGYPGGAIMP+YDALY VEH LCRHEQGA AA+GYARA Sbjct: 6 MRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAVGYARA 65 Query: 61 TGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAPFIGTDAFQEVDVLGLSLA 120 +GKTGVC ATSGPGATNLIT LADALLDS+PVVAITGQVS IGTDAFQE+DVLG+SL+ Sbjct: 66 SGKTGVCFATSGPGATNLITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVLGMSLS 125 Query: 121 CTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKDIQLASGDLEPWFTTVENEVT 180 CTKHSF+VQ+++EL + AF++A SGRPGPVLVDIPKDIQ+A + V +E Sbjct: 126 CTKHSFMVQTVDELVPTLYRAFELAASGRPGPVLVDIPKDIQIAKLEYRAPLLAVADEPK 185 Query: 181 FPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALREFLAATKMPATCTLKGLGAVEAD 240 A+++ AR ++A A+KPMLYVGGGVGMA AV LR F+ AT MP+ TLKGLGA+ Sbjct: 186 VQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPSVATLKGLGAIPHG 245 Query: 241 YPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDRVTGKLNTFAPHASVIHMDIDPAEM 300 P YLGMLGMHG KAAN AVQECDLL+ VGARFDDRVTG+L +FAP+A V+H+DID AE+ Sbjct: 246 TPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDIDAAEL 305 Query: 301 NKLRQAHVALQGDLNALLPALQQPLNQYDWQQHCAQLRDEHSWRYDHPGDAIYAPLLLKQ 360 KLRQ VA+ +L +LP L+ L+ W+ L EH W Y+HPG IYAP +L++ Sbjct: 306 GKLRQPDVAIAAELRVVLPMLEMQLDIDPWRAEVEALAAEHRWDYNHPGSLIYAPAMLRR 365 Query: 361 LSDRKPADCVVTTDVGQHQMWAAQHIAHTRPENFITSSGLGTMGFGLPAAVGAQVARPND 420 L+++ P D VV+ DVGQHQMW AQH+ RPE+ ++S+GLGTMGFGLPAA+GAQ+ARP+ Sbjct: 366 LANKLPEDSVVSCDVGQHQMWVAQHMHFRRPEDHLSSAGLGTMGFGLPAAIGAQMARPDA 425 Query: 421 TVVCISGDGSFMMNVQELGTVKRKQLPLKIVLLDNQRLGMVRQWQQLFFQERYSETTLTD 480 TVV +SGDGSFMMNVQEL T+KR++LP+KI+L+DNQRLGMV+QWQQLFF+ERYSET L+D Sbjct: 426 TVVAVSGDGSFMMNVQELTTIKRRKLPVKILLIDNQRLGMVKQWQQLFFEERYSETNLSD 485 Query: 481 NPDFLMLASAFGIHGQHITRKDQVEAALDTMLNSDGPYLLHVSIDELENVWPLVPPGASN 540 NPDF+ LASAF I G+ I ++VE AL ML S GPYLLHV+ID+ NVWPLVPPGASN Sbjct: 486 NPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFNVWPLVPPGASN 545 Query: 541 SEMLEKL 547 S+M+E++ Sbjct: 546 SDMMEEM 552 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 899 Number of extensions: 26 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 548 Length of database: 556 Length adjustment: 36 Effective length of query: 512 Effective length of database: 520 Effective search space: 266240 Effective search space used: 266240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
Align candidate 6939187 Sama_3281 (acetolactate synthase 2 catalytic subunit (RefSeq))
to HMM TIGR00118 (ilvB: acetolactate synthase, large subunit, biosynthetic type (EC 2.2.1.6))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00118.hmm # target sequence database: /tmp/gapView.8064.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00118 [M=557] Accession: TIGR00118 Description: acolac_lg: acetolactate synthase, large subunit, biosynthetic type Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.5e-213 694.3 0.1 6.4e-213 694.1 0.1 1.0 1 lcl|FitnessBrowser__SB2B:6939187 Sama_3281 acetolactate synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6939187 Sama_3281 acetolactate synthase 2 catalytic subunit (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 694.1 0.1 6.4e-213 6.4e-213 1 556 [. 6 551 .. 6 552 .. 0.97 Alignments for each domain: == domain 1 score: 694.1 bits; conditional E-value: 6.4e-213 TIGR00118 1 lkgaeilveslkkegvetvfGyPGGavlpiydalydselehilvrheqaaahaadGyarasGkvGvvlatsGPGatn 77 ++ga+++++ l ++gv+tvfGyPGGa++piydaly se+eh l rheq+a aa GyarasGk+Gv++atsGPGatn lcl|FitnessBrowser__SB2B:6939187 6 MRGADAVIKVLAAHGVNTVFGYPGGAIMPIYDALYGSEVEHTLCRHEQGAGFAAVGYARASGKTGVCFATSGPGATN 82 79*************************************************************************** PP TIGR00118 78 lvtgiatayldsvPlvvltGqvatsliGsdafqeidilGitlpvtkhsflvkkaedlpeilkeafeiastGrPGPvl 154 l+t++a+a ldsvP+v++tGqv+ts+iG+dafqeid+lG++l++tkhsf+v+ +++l +l +afe+a++GrPGPvl lcl|FitnessBrowser__SB2B:6939187 83 LITALADALLDSVPVVAITGQVSTSVIGTDAFQEIDVLGMSLSCTKHSFMVQTVDELVPTLYRAFELAASGRPGPVL 159 ***************************************************************************** PP TIGR00118 155 vdlPkdvteaeieleveekvelpgykptvkghklqikkaleliekakkPvllvGgGviiaeaseelkelaerlkipv 231 vd+Pkd++ a++e++ + l + + + k + + i +a li++akkP+l+vGgGv +a+a e l+++ +++ +p lcl|FitnessBrowser__SB2B:6939187 160 VDIPKDIQIAKLEYRAP----LLAVADEPKVQDADIDAARALIAAAKKPMLYVGGGVGMAGAVEPLRHFIKATYMPS 232 ********999999887....555555556678899***************************************** PP TIGR00118 232 tttllGlGafpedhplalgmlGmhGtkeanlavseadlliavGarfddrvtgnlakfapeakiihididPaeigknv 308 ++tl GlGa+p+ p lgmlGmhG k+anlav+e+dll+ vGarfddrvtg la+fap+ak++h+did ae+gk + lcl|FitnessBrowser__SB2B:6939187 233 VATLKGLGAIPHGTPGYLGMLGMHGGKAANLAVQECDLLMVVGARFDDRVTGRLASFAPNAKVLHLDIDAAELGKLR 309 ***************************************************************************** PP TIGR00118 309 kvdipivGdakkvleellkklkeeekkekeWlekieewkkeyilkldeeeesikPqkvikelskllkdeaivttdvG 385 + d++i + + vl l +l + W +++e + +e+ +++ + i +++++l + l+++++v+ dvG lcl|FitnessBrowser__SB2B:6939187 310 QPDVAIAAELRVVLPMLEMQLD---ID--PWRAEVEALAAEHRWDYNHPGSLIYAPAMLRRLANKLPEDSVVSCDVG 381 ************9987755442...22..3*************99**999999999********************* PP TIGR00118 386 qhqmwaaqfyktkkprkfitsgGlGtmGfGlPaalGakvakpeetvvavtGdgsfqmnlqelstiveydipvkivil 462 qhqmw+aq+ ++++p+ +++s+GlGtmGfGlPaa+Ga++a p++tvvav+Gdgsf+mn+qel+ti++ ++pvki+++ lcl|FitnessBrowser__SB2B:6939187 382 QHQMWVAQHMHFRRPEDHLSSAGLGTMGFGLPAAIGAQMARPDATVVAVSGDGSFMMNVQELTTIKRRKLPVKILLI 458 ***************************************************************************** PP TIGR00118 463 nnellGmvkqWqelfyeerysetklaselpdfvklaeayGvkgiriekpeeleeklkealeskepvlldvevdkeee 539 +n+ lGmvkqWq+lf+eeryset+l+ ++pdfv+la a+ + g +i ee+ee+l+e+l+sk+p+ll v +d+ + lcl|FitnessBrowser__SB2B:6939187 459 DNQRLGMVKQWQQLFFEERYSETNLS-DNPDFVALASAFDIPGRTIFAAEEVEEALTEMLTSKGPYLLHVAIDDAFN 534 **************************.6************************************************* PP TIGR00118 540 vlPmvapGagldelvee 556 v+P+v+pGa++++++ee lcl|FitnessBrowser__SB2B:6939187 535 VWPLVPPGASNSDMMEE 551 *************9975 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (557 nodes) Target sequences: 1 (556 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 12.50 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory