Align 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7) (characterized)
to candidate 6937767 Sama_1908 dihydrodipicolinate synthase (RefSeq)
Query= BRENDA::A5F699 (292 letters) >FitnessBrowser__SB2B:6937767 Length = 293 Score = 344 bits (882), Expect = 2e-99 Identities = 169/289 (58%), Positives = 217/289 (75%) Query: 1 MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVV 60 M +GSIVALITP DG VD+ SL+ LV+FH+ GTDAIV+VGTTGESATL ++EH+ VV Sbjct: 1 MINGSIVALITPMNSDGSVDFASLESLVEFHIANGTDAIVAVGTTGESATLPMKEHINVV 60 Query: 61 AKTVEFAEGRLPIIAGTGANATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYN 120 AKTVEFA+GR+P+I G GANAT EA+ ++ L+ TG+A L VTPYYNKPT +GL HY Sbjct: 61 AKTVEFAKGRIPVIGGNGANATAEAIELTQALSATGVAAMLGVTPYYNKPTPKGLVAHYK 120 Query: 121 AIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVALKDATGDLSRVAKHREMCKEG 180 A+A TDIP ILYNVPGRTA+D++PETVA L ++ NI+ +K+ATGD+SRVA+ RE+C Sbjct: 121 AVAAATDIPQILYNVPGRTALDLKPETVAELCDVANIIGIKEATGDVSRVARLRELCGPD 180 Query: 181 FVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH 240 F+L SGDDAT EF+ GG GVISV NNI A KM LAL G + + + ++ + L+ Sbjct: 181 FLLYSGDDATAREFLLAGGDGVISVANNIVPAQFKKMCALALAGDIEGSLAADEPMRGLY 240 Query: 241 KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEA 289 LF E++PIPVKWAAH+MGLI G +RLPLT+LSE ++ A+ +A Sbjct: 241 STLFCEANPIPVKWAAHRMGLIKEGFIRLPLTELSESCHGLLLDAMKKA 289 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 327 Number of extensions: 8 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 292 Length of database: 293 Length adjustment: 26 Effective length of query: 266 Effective length of database: 267 Effective search space: 71022 Effective search space used: 71022 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
Align candidate 6937767 Sama_1908 (dihydrodipicolinate synthase (RefSeq))
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00674.hmm # target sequence database: /tmp/gapView.6087.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00674 [M=286] Accession: TIGR00674 Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2.2e-110 354.0 0.0 2.5e-110 353.8 0.0 1.0 1 lcl|FitnessBrowser__SB2B:6937767 Sama_1908 dihydrodipicolinate sy Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SB2B:6937767 Sama_1908 dihydrodipicolinate synthase (RefSeq) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 353.8 0.0 2.5e-110 2.5e-110 1 284 [. 4 287 .. 4 289 .. 0.98 Alignments for each domain: == domain 1 score: 353.8 bits; conditional E-value: 2.5e-110 TIGR00674 1 gvltAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvknrvpviaGtgsn 77 g+++AliTP+++dgsvdfa+le+l+e++i++g+daiv+vGtTGEsatL ++E+++v+ ++ve++k+r+pvi G g+n lcl|FitnessBrowser__SB2B:6937767 4 GSIVALITPMNSDGSVDFASLESLVEFHIANGTDAIVAVGTTGESATLPMKEHINVVAKTVEFAKGRIPVIGGNGAN 80 589************************************************************************** PP TIGR00674 78 ateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfkaiaeevelPiilYnvPsRtgvslepetvkrLaeevei 154 at+eaielt+ ++ +gv+++l vtPyYnkPt +Gl++h+ka+a+++++P+ilYnvP+Rt+ +l+petv++L + +i lcl|FitnessBrowser__SB2B:6937767 81 ATAEAIELTQALSATGVAAMLGVTPYYNKPTPKGLVAHYKAVAAATDIPQILYNVPGRTALDLKPETVAELCDVANI 157 ***************************************************************************** PP TIGR00674 155 vaiKeasgdlervseikaeakedfkvlsGdDaltleilalGakGviSVasnvapkelkemvkaalegdteeareihq 231 ++iKea+gd++rv ++++ + df ++sGdDa++ e+l+ G+ GviSVa+n++p ++k+m++ al+gd+e + ++ lcl|FitnessBrowser__SB2B:6937767 158 IGIKEATGDVSRVARLRELCGPDFLLYSGDDATAREFLLAGGDGVISVANNIVPAQFKKMCALALAGDIEGSLAADE 234 ***************************************************************************** PP TIGR00674 232 kllklfkalfietNPipvKtalallgliekdelRlPLtelseekkeklkevlk 284 + l+++lf+e+NPipvK+a++ +gli++ +RlPLtelse+ + l +++k lcl|FitnessBrowser__SB2B:6937767 235 PMRGLYSTLFCEANPIPVKWAAHRMGLIKEGFIRLPLTELSESCHGLLLDAMK 287 ******************************************98877777666 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (286 nodes) Target sequences: 1 (293 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 6.97 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory