Alignments for a candidate for trpA in Shewanella amazonensis SB2B

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpA in Shewanella amazonensis SB2B

Align tryptophan synthase subunit α (EC 4.1.2.8) (characterized)
to candidate 6938001 Sama_2134 tryptophan synthase subunit alpha (RefSeq)

Query= metacyc::MONOMER-3561
         (251 letters)



>FitnessBrowser__SB2B:6938001
          Length = 266

 Score =  157 bits (398), Expect = 2e-43
 Identities = 84/250 (33%), Positives = 144/250 (57%), Gaps = 5/250 (2%)

Query: 4   KGSLIPYLTAGDPSVEKTLEFLLA-VEEFAGLIELGIPFSDPMADGKTIQESHYRALRNG 62
           +G+ +P++T GDP ++++L  + A VE  A  +ELG PFSDP+ADG  IQ ++ RAL  G
Sbjct: 16  RGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLADGPVIQGANIRALAAG 75

Query: 63  FKLDDTFRILREFR-RHSSTPVILMTYYNPVFRTGVKKFLGEAKASGADGILVVDLPVSH 121
              D  F+++ + R ++   P+ L+ Y N VF  G++ F   AKA+G D +L+ D+P   
Sbjct: 76  TTPDTCFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKAAGVDSVLIADVPAEE 135

Query: 122 AGEFLDAAKEEGLKTVFLAAPNTPDERLREIDKASTGFVYLISLYGTTGARDRLPETAFE 181
           +  F+ AA+ EG+  +F+A PN   + LR +     G+ YL+S  G TGA ++       
Sbjct: 136 SAPFVAAARAEGIAPIFIAPPNASADTLRLVASLGEGYTYLLSRAGVTGADNKAGMPLDS 195

Query: 182 FVRRARKICNNKLAVGFGVSRREQVEELLKAGADGVVVGSALIELISRSENPVEELRRKV 241
            +   ++       +GFG++   QV+E + AGA G + GSA+++   R E   +++ + +
Sbjct: 196 VLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVK---RIEALKDDMPKLL 252

Query: 242 AELSGYSRAL 251
            EL  ++ A+
Sbjct: 253 TELKSFASAM 262


Lambda     K      H
   0.319    0.138    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 188
Number of extensions: 8
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 251
Length of database: 266
Length adjustment: 24
Effective length of query: 227
Effective length of database: 242
Effective search space:    54934
Effective search space used:    54934
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

Align candidate 6938001 Sama_2134 (tryptophan synthase subunit alpha (RefSeq))
to HMM TIGR00262 (trpA: tryptophan synthase, alpha subunit (EC 4.2.1.20))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00262.hmm
# target sequence database:        /tmp/gapView.8025.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00262  [M=256]
Accession:   TIGR00262
Description: trpA: tryptophan synthase, alpha subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                         Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                         -----------
    2.5e-99  317.3   0.0    2.9e-99  317.1   0.0    1.0  1  lcl|FitnessBrowser__SB2B:6938001  Sama_2134 tryptophan synthase su


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__SB2B:6938001  Sama_2134 tryptophan synthase subunit alpha (RefSeq)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  317.1   0.0   2.9e-99   2.9e-99       1     256 []       8     263 ..       8     263 .. 0.99

  Alignments for each domain:
  == domain 1  score: 317.1 bits;  conditional E-value: 2.9e-99
                         TIGR00262   1 fetlkkkeekafvpFvtagdPdleksleiiktlvkaGadalElGvpfsDPlaDGptiqaaelRAlkagvkvekalel 77 
                                       f+ lk+ +  afvpFvt+gdP +++sl ii+ lv+ Gad lElG+pfsDPlaDGp+iq a+ RAl+ag++++ ++++
  lcl|FitnessBrowser__SB2B:6938001   8 FARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLADGPVIQGANIRALAAGTTPDTCFKM 84 
                                       678999999******************************************************************** PP

                         TIGR00262  78 lkkvrekasniPivlltyynlifkkgveeFyakakeagvdgvlvaDlPleeaddlleaakkhgvkqiflvaPtaeee 154
                                       ++++r+k+++ Pi+ll+y+nl+f++g+e+Fy++ak+agvd+vl+aD+P ee++ ++ aa+ +g+++if+++P+a+ +
  lcl|FitnessBrowser__SB2B:6938001  85 IEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKAAGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASAD 161
                                       ***************************************************************************** PP

                         TIGR00262 155 rlkkiaekseGfvYlvsvaGvtgarerveeevkelikkvkalskkPvlvGFGiskkeqvkelkelgadgvivGsAlv 231
                                       +l+ +a+ +eG++Yl+s+aGvtga +++   +++++  +k++++ P l+GFGi+++ qv+e++++ga+g+i+GsA+v
  lcl|FitnessBrowser__SB2B:6938001 162 TLRLVASLGEGYTYLLSRAGVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVV 238
                                       ***************************************************************************** PP

                         TIGR00262 232 kiieeklddeekaleeleefvkelk 256
                                       k+ie  +dd  k+l el++f++++k
  lcl|FitnessBrowser__SB2B:6938001 239 KRIEALKDDMPKLLTELKSFASAMK 263
                                       *********************9997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (256 nodes)
Target sequences:                          1  (266 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 6.26
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis