Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate SMc00155 SMc00155 DAHP synthetase prtein
Query= BRENDA::P29976 (525 letters) >FitnessBrowser__Smeli:SMc00155 Length = 457 Score = 531 bits (1367), Expect = e-155 Identities = 260/454 (57%), Positives = 330/454 (72%), Gaps = 2/454 (0%) Query: 72 ALKWTPESWKLKKALQLPDYPNANELESVLKTIEAFPPIVFAGEARNLEERLADAAVGKA 131 A WTP SW+ K Q+PDYP+ L+SV + +PP+VFAGEAR L+ LA+ A G+ Sbjct: 2 AQTWTPNSWRQKPIQQVPDYPDLAVLDSVEARLAKYPPLVFAGEARRLKSALANVAEGRG 61 Query: 132 FLLQGGDCAESFKEFNATNIRDTFRVLLQMSIVLTFGGQVPVIKVGRMAGQFAKPRSDAF 191 FLLQGGDCAESF E A IRD FR LQM++VLTFG Q PV+KVGR+AGQFAKPRS Sbjct: 62 FLLQGGDCAESFAEHGADTIRDFFRAFLQMAVVLTFGAQQPVVKVGRIAGQFAKPRSSGV 121 Query: 192 EEKDGVKLPSYKGDNINGDTFDEKSRIPDPNRMIRAYTQSAATLNLLRAFATGGYAAIQR 251 E++ V LPSY+GD ING F E++R+P+P R + AY QSAATLNLLRAFA GGYA ++ Sbjct: 122 EKQGDVTLPSYRGDIINGIEFTEEARVPNPERQVMAYRQSAATLNLLRAFAMGGYANLEN 181 Query: 252 VTQWNLDFVEQSEQADRYQELANRVDEALGFMSACGL-GTDHPLMTTTDFYTSHECLLLP 310 V QW L FV+ S QA+RY++LA+R+ E + FM A G+ +HP + TDF+TSHE LLL Sbjct: 182 VHQWMLGFVKDSPQAERYRKLADRISETMDFMKAIGITAENHPSLRETDFFTSHEALLLG 241 Query: 311 YEQSLTRLDSTSGLYYDCSAHMVWCGERTRQLDGAHVEFLRGIANPLGIKVSNKMDPFEL 370 YEQ+LTR+DSTSG +Y S HM+W G+RTRQ D AH+E+ RGI NPLG+K + L Sbjct: 242 YEQALTRVDSTSGDWYATSGHMIWIGDRTRQPDHAHIEYCRGIKNPLGLKCGPSLTADGL 301 Query: 371 VKLVEILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRRSGQIVTWVCDPMHGNTIKAP 430 ++L++ILNP N+ GR+T+I R G + + LP LIRAV R G+ V W CDPMHGNTI Sbjct: 302 LELIDILNPANEAGRLTLICRFGHDKVAEHLPRLIRAVEREGKKVVWSCDPMHGNTITLN 361 Query: 431 CGLKTRAFDSILAEVRAFLDVHEQEGSHAGGIHLEMTGQNVTECIGGSRTVTYDDLSSRY 490 KTR F+ IL+EV +F +H EGSH GGIH+EMTG +VTEC GG+R ++ DDL+ RY Sbjct: 362 -NYKTRPFERILSEVESFFQIHRAEGSHPGGIHIEMTGNDVTECTGGARALSGDDLADRY 420 Query: 491 HTHCDPRLNASQSLELAFIVAERLRKRRTGSQRV 524 HTHCDPRLNA Q+LELAF++AER++ R + V Sbjct: 421 HTHCDPRLNADQALELAFLLAERMKGGRDEKKMV 454 Lambda K H 0.318 0.132 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 651 Number of extensions: 25 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 525 Length of database: 457 Length adjustment: 34 Effective length of query: 491 Effective length of database: 423 Effective search space: 207693 Effective search space used: 207693 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate SMc00155 SMc00155 (DAHP synthetase prtein)
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01358.hmm # target sequence database: /tmp/gapView.15022.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01358 [M=443] Accession: TIGR01358 Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-216 704.0 0.0 3.5e-216 703.8 0.0 1.0 1 lcl|FitnessBrowser__Smeli:SMc00155 SMc00155 DAHP synthetase prtein Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Smeli:SMc00155 SMc00155 DAHP synthetase prtein # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 703.8 0.0 3.5e-216 3.5e-216 1 440 [. 5 445 .. 5 448 .. 0.99 Alignments for each domain: == domain 1 score: 703.8 bits; conditional E-value: 3.5e-216 TIGR01358 1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGdcaesfkevead 75 w+++swr+kp +q+P+yPd + l++v++ l+++PPlv+age+++lk+ la+va+G++fllqgGdcaesf e ad lcl|FitnessBrowser__Smeli:SMc00155 5 WTPNSWRQKPIQQVPDYPDLAVLDSVEARLAKYPPLVFAGEARRLKSALANVAEGRGFLLQGGDCAESFAEHGAD 79 89************************************************************************* PP TIGR01358 76 nirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsyrGdvingaafdeaarvpdperl 150 +ird +r++lqmavvlt+ga+ PvvkvgriaGq+akPrs+ +e++++vtlpsyrGd+ing +f+e+arvp+per lcl|FitnessBrowser__Smeli:SMc00155 80 TIRDFFRAFLQMAVVLTFGAQQPVVKVGRIAGQFAKPRSSGVEKQGDVTLPSYRGDIINGIEFTEEARVPNPERQ 154 *************************************************************************** PP TIGR01358 151 vrayaksaatlnllraltsgGyadlkkvheWnlefvkkspagaryeklaeeidealrfmsavgvaeae..alkrv 223 v ay +saatlnllra++ gGya+l++vh+W l fvk+sp+++ry+kla++i+e++ fm+a+g++ ++ +l+++ lcl|FitnessBrowser__Smeli:SMc00155 155 VMAYRQSAATLNLLRAFAMGGYANLENVHQWMLGFVKDSPQAERYRKLADRISETMDFMKAIGITAENhpSLRET 229 ***************************************************************99888799**** PP TIGR01358 224 elytsheallldyeealtrvdskegqlfdlsahllWiGertrqldgahveflrgvknPigikvgpsmeadellkl 298 +++tshealll ye+altrvds++g+++ +s+h++WiG+rtrq d+ah+e+ rg+knP+g+k+gps++ad ll+l lcl|FitnessBrowser__Smeli:SMc00155 230 DFFTSHEALLLGYEQALTRVDSTSGDWYATSGHMIWIGDRTRQPDHAHIEYCRGIKNPLGLKCGPSLTADGLLEL 304 *************************************************************************** PP TIGR01358 299 ievldPenePGrltlisrlGaekiaeklPelleavkaaGravvWvtdpmhGntleaasGyktrrfddilsevkef 373 i++l+P ne Grltli r+G +k+ae+lP+l++av+++G++vvW +dpmhGnt++ + yktr f++ilsev++f lcl|FitnessBrowser__Smeli:SMc00155 305 IDILNPANEAGRLTLICRFGHDKVAEHLPRLIRAVEREGKKVVWSCDPMHGNTIT-LNNYKTRPFERILSEVESF 378 *****************************************************96.577**************** PP TIGR01358 374 fevhkaeGthpGGvhleltGedvteclGGareitetdlasryetacdPrlnaeqslelaflvaeklr 440 f++h+aeG+hpGG+h+e+tG+dvtec+GGar+++ +dla+ry+t+cdPrlna+q+lelafl+ae+++ lcl|FitnessBrowser__Smeli:SMc00155 379 FQIHRAEGSHPGGIHIEMTGNDVTECTGGARALSGDDLADRYHTHCDPRLNADQALELAFLLAERMK 445 ****************************************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (457 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.43 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory