Aligments for a candidate for hom in Sinorhizobium meliloti 1021

GapMind for Amino acid biosynthesis

Aligments for a candidate for hom in Sinorhizobium meliloti 1021

Align Homoserine dehydrogenase; EC 1.1.1.3 (characterized, see rationale)
to candidate SMc00293 SMc00293 homoserine dehydrogenase

Query= uniprot:A0A1L6J6Q3
         (430 letters)



>lcl|FitnessBrowser__Smeli:SMc00293 SMc00293 homoserine
           dehydrogenase
          Length = 440

 Score =  375 bits (962), Expect = e-108
 Identities = 203/434 (46%), Positives = 275/434 (63%), Gaps = 7/434 (1%)

Query: 1   MTEPLRVALAGLGTVGAGVIRLIDANAELIARRAGRPIEIVAVSARDRAKDRGVDITRFD 60
           M + L++ +AGLGTVGA ++R++    E +A   GR IEI AV+ARDR+KDRGV +T   
Sbjct: 1   MADALKIGIAGLGTVGASLVRILQDRHETMATTCGRAIEITAVAARDRSKDRGVKLTGIA 60

Query: 61  WVDDMTELARHPKADVVVELIGGSDGPALALARATLAAGKGLVTANKAMIAHHGLELAQV 120
           W +D   LA     DV VEL+GG+  PA +  +A L  G  +VTANKA++A HG+ELA++
Sbjct: 61  WFEDAVSLASEADVDVFVELMGGAGDPAYSCVKAALTRGVHVVTANKALLAAHGIELAEI 120

Query: 121 AEKSDTPMKFEAAVAGGVPVIKGLREGAAANQIDRVYGILNGTCNFILSKMEAEGRDFGE 180
           AE+    + +EAAVAGG+PVIK LRE    N I RVYGI+NGTCN+IL+KME EG  F E
Sbjct: 121 AEQHGALLNYEAAVAGGIPVIKALRESMTGNTISRVYGIMNGTCNYILTKMEKEGLSFEE 180

Query: 181 VLAEAQAAGFAEADPSFDIDGVDAAHKLSILASIAFGTQPAFGDVAIGGIRHLLAADIAE 240
            L EAQ  G+AEADP+FDI+G D AHKLSIL S+AFGT  A  D+ + GI ++   DI  
Sbjct: 181 CLKEAQRLGYAEADPAFDIEGNDTAHKLSILTSLAFGTAIAADDIYLEGITNISIEDIQA 240

Query: 241 AAALGYRIRLLGIADLSGNGLFQRVHPHLVPLSHPLAHVLGPTNAVVAEGNFVGRLLFQG 300
           A+ LGYRI+LLG+A  + +G+ QRVHP +VP    +A V G TNAV  E + +G LL  G
Sbjct: 241 ASDLGYRIKLLGVAQRTDSGIEQRVHPTMVPHDTVIAQVDGVTNAVAIESDILGELLMVG 300

Query: 301 AGAGDGPTASAVVADLIDIARTEFG----PPYAMPATSLAAEPVAPTGERRGRAYLRFTV 356
            GAG   TASAV+ D+ DIA++  G    P +  PA +L     A      G  ++R  V
Sbjct: 301 PGAGGDATASAVLGDIADIAKSRPGAQHVPAFGRPAKALLPYKRARMQSHEGGYFIRLKV 360

Query: 357 ADKVGVLAEIAAAMRDAGVSIESLIQRGA-MADGS--VLVAIVTHEVPERSIAQALEKLR 413
            D+ GV A +A  M +  +S+ES++Q     AD +    + +VTH   E S+ +A+  ++
Sbjct: 361 VDRTGVFANVAKHMAENDISLESIMQHSKHYADPAEPKTIILVTHATSEASVRKAIVSIK 420

Query: 414 GSPSLAGEPMWMHI 427
           G   L GEP  + I
Sbjct: 421 GEGYLVGEPQVIRI 434


Lambda     K      H
   0.319    0.136    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 466
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 440
Length adjustment: 32
Effective length of query: 398
Effective length of database: 408
Effective search space:   162384
Effective search space used:   162384
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis