Align candidate SMc03858 SMc03858 (chorismate mutase)
to HMM TIGR01795 (chorismate mutase (EC 5.4.99.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01795.hmm # target sequence database: /tmp/gapView.4545.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01795 [M=94] Accession: TIGR01795 Description: CM_mono_cladeE: chorismate mutase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-52 162.9 1.3 1.3e-52 162.7 1.3 1.1 1 lcl|FitnessBrowser__Smeli:SMc03858 SMc03858 chorismate mutase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Smeli:SMc03858 SMc03858 chorismate mutase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 162.7 1.3 1.3e-52 1.3e-52 1 92 [. 6 97 .. 6 99 .. 0.98 Alignments for each domain: == domain 1 score: 162.7 bits; conditional E-value: 1.3e-52 TIGR01795 1 vvaelkalrdsidnidaaviallaerfkatkqvGvlkaeaglaPadparedrqiarlralaadakldPefaekflnf 77 +++el++ r+sidnidaa++++laerf++tk+vGvlka+++l+Padpare++qi+rlr la+da+ldP+faekflnf lcl|FitnessBrowser__Smeli:SMc03858 6 IKEELASYRQSIDNIDAALVHMLAERFRCTKAVGVLKAKHQLPPADPAREEYQIERLRHLAKDANLDPDFAEKFLNF 82 689************************************************************************** PP TIGR01795 78 ivkevikrheriaea 92 i+kevi++he ia+ lcl|FitnessBrowser__Smeli:SMc03858 83 IIKEVIRHHEAIAAD 97 ************975 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (94 nodes) Target sequences: 1 (111 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 4.95 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory