GapMind for Amino acid biosynthesis

 

Protein Synpcc7942_1896 in Synechococcus elongatus PCC 7942

Annotation: FitnessBrowser__SynE:Synpcc7942_1896

Length: 414 amino acids

Source: SynE in FitnessBrowser

Candidate for 4 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine biosynthesis argA hi Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 (characterized) 56% 88% 439.1 Glutamate N-acetyltransferase; Ornithine acetyltransferase; OATase; Ornithine transacetylase; EC 2.3.1.35 45% 307.8
L-arginine biosynthesis argJ hi Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 (characterized) 56% 88% 439.1
L-proline biosynthesis argA hi Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 (characterized) 56% 88% 439.1 Glutamate N-acetyltransferase; Ornithine acetyltransferase; OATase; Ornithine transacetylase; EC 2.3.1.35 45% 307.8
L-proline biosynthesis argJ hi Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 (characterized) 56% 88% 439.1

Sequence Analysis Tools

View Synpcc7942_1896 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MQAAWQEISGGLTAPRGFQAAGITAGLKASGQPDLALIVSESDAIAAAVFTTSQVRAACV
DFSRQQLDGNSIARAILCNSGQANAATGDQGWSDAVESAEQLAQALQIPSHQVLVASTGV
IGQRIKMEALRTGIPQAIAALSPEGGPAAAQAILTTDLVAKQIALELPLGDRTVRIGGIA
KGSGMIHPNMATMLSFITCDAAVSPQLWQEMLSRAVDRSFNQITVDGDTSTNDCVFALAN
GQSRTPAITERGPIADQLEAMLTAVCQHLAKAIARDGEGATCLIEVQVKGTADDAAARAI
ARTIAGSSLVKSAIFGRDPNWGRIAAAAGRAGVQFNSENLGVRLGEFELLRNGQPLPFDR
NAASQYLRDRAAGAYLQDDTVLIQVDVGAGTGQGVAWGCDLSYDYVRINAEYTT

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory