Alignments for a candidate for gltX in Synechococcus elongatus PCC 7942

GapMind for Amino acid biosynthesis

Alignments for a candidate for gltX in Synechococcus elongatus PCC 7942

Align Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 (characterized)
to candidate Synpcc7942_2393 Synpcc7942_2393 glutamyl-tRNA synthetase

Query= SwissProt::Q8DLI5
         (485 letters)



>FitnessBrowser__SynE:Synpcc7942_2393
          Length = 481

 Score =  614 bits (1583), Expect = e-180
 Identities = 309/482 (64%), Positives = 366/482 (75%), Gaps = 2/482 (0%)

Query: 1   MTVRVRLAPSPTGNLHIGTARTAVFNWLYARHRGGKFILRIEDTDRERSRPEYTENILEG 60
           ++VRVR+APSPTGNLHIGTARTAVFNWL+AR   G+FILRIEDTD ERSR EYT+NIL G
Sbjct: 1   VSVRVRIAPSPTGNLHIGTARTAVFNWLFARRHQGQFILRIEDTDLERSRSEYTDNILTG 60

Query: 61  LQWLGLTWDEGPYFQSDRLDLYRQAIQTLLDKGLAYYCYCTPEELEALRAEQKAKGQAPR 120
           LQWLGL WDEGP++Q+ RLDLY+ A+Q LLD G AY CYCT  ELEALR  Q+A+ +APR
Sbjct: 61  LQWLGLNWDEGPFYQTQRLDLYKAAVQQLLDSGKAYRCYCTEAELEALRESQRARNEAPR 120

Query: 121 YDNRHRHLTPEEQAAFEAAGRTPVIRFKIEDDRQIEWQDLVRGRVSWQGADLGGDMVIAR 180
           YDNRHR LTPE++AAF+A GR  VIRF+I+DDR+I W DLVR RV W+G+DLGGDMVIAR
Sbjct: 121 YDNRHRDLTPEQEAAFQAEGREAVIRFRIDDDREIAWTDLVRDRVVWKGSDLGGDMVIAR 180

Query: 181 AAPRGEIGYPLYNLVVVVDDIAMGITDVIRGEDHIGNTPKQILLYEALGATPPNFAHTPL 240
            +P G IG PLYNL VVVDDI M I+ VIRGEDHI NT KQILLYEALGA  P FAHTPL
Sbjct: 181 RSPAGTIGQPLYNLAVVVDDIDMTISHVIRGEDHIANTAKQILLYEALGAAVPEFAHTPL 240

Query: 241 ILNSTGQKLSKRDGVTSISDFRAMGYLAPALANYMTLLGWSPPEGVGELFTLDLAAKHFS 300
           ILN  G+KLSKRDGVTSISDF+ +GYL  A+ANYMTLLGWSP EG+ E F+L  AA  F 
Sbjct: 241 ILNKEGRKLSKRDGVTSISDFQNLGYLPEAIANYMTLLGWSPVEGMDERFSLAEAATVFD 300

Query: 301 FERINKAGARFDWDKLNWLNRQYIQQLEPEEFLAELIPLWQGAGYAFDEERDRPWLFDLA 360
           F+R+NKAGA+FDWDKLNWLN Q I++    E +A L P W  AG          WL +LA
Sbjct: 301 FDRVNKAGAKFDWDKLNWLNSQVIKEKSASELVALLQPFWSKAG-VDTAAYPAAWLEELA 359

Query: 361 QLLQPGLNTLREAIDQGAVFFIPSVTFDSEAMAQLGQPQSATILAYLLEHLPAEPALTVA 420
            LL P L TL + + Q  +FF   +    +A+AQLGQ  S  +L  +LE LP+E ALT+ 
Sbjct: 360 TLLGPSLVTLTDIVGQSQLFFSQGIELQEDAIAQLGQAGSKAVLQQILEALPSE-ALTLE 418

Query: 421 MGQQLIQQAAKAAGVKKGATMRTLRAALTGAVHGPDLMAAWQILHQRGWDEPRLAAALKQ 480
           + + LI QA KAAGVKKG  MR+LRAAL G++ GPDL+ +W +LHQ G  +PRL AA+  
Sbjct: 419 VAKGLIDQAVKAAGVKKGIGMRSLRAALMGSMQGPDLLTSWVLLHQAGQAQPRLQAAIAA 478

Query: 481 AQ 482
           AQ
Sbjct: 479 AQ 480


Lambda     K      H
   0.320    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 740
Number of extensions: 30
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 481
Length adjustment: 34
Effective length of query: 451
Effective length of database: 447
Effective search space:   201597
Effective search space used:   201597
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis