GapMind for Amino acid biosynthesis

 

Aligments for a candidate for proC in Synechococcus elongatus PCC 7942

Align Pyrroline-5-carboxylate reductase; P5C reductase; P5CR; PCA reductase; EC 1.5.1.2 (characterized)
to candidate Synpcc7942_2058 Synpcc7942_2058 pyrroline-5-carboxylate reductase

Query= SwissProt::Q9HH99
         (270 letters)



>lcl|FitnessBrowser__SynE:Synpcc7942_2058 Synpcc7942_2058
           pyrroline-5-carboxylate reductase
          Length = 264

 Score =  205 bits (522), Expect = 7e-58
 Identities = 115/263 (43%), Positives = 162/263 (61%), Gaps = 3/263 (1%)

Query: 6   IGFIGAGKMGSALMQGTIKAGIVTPENIGASDVYEPFLKDLQAKLGIRVSTDNAVIVRES 65
           +G IG G MG AL+   +  G V    +  S+ +    + L  + G+R   DNA    ++
Sbjct: 5   LGIIGGGVMGEALLARLLAQGQVAATAVTVSEPFAARCEFLNDRYGVRCVDDNAEAA-QA 63

Query: 66  DILILAVKPQTLSSVLSNLKNEITSEKLVISIAAGVPLSTYEDALLEGTRVVRVMPNIAA 125
           D ++LA+KPQ     ++ L  ++ S++L++SI AGVPL+  E A      V+R MPN  A
Sbjct: 64  DWVLLAIKPQVFDPAVAGLA-DLRSDRLILSILAGVPLAKLE-AAFPHEAVIRAMPNTPA 121

Query: 126 TVSEAASGIAPGKNATPEDLKAALEIFSAVGTAVQVPESLMDAVTGLSGSGPAFIFPVIE 185
           TV    + IA G+  T + L  A E F+AVG  V VPES +DAVTGLSGSGP ++  ++E
Sbjct: 122 TVGAGVTAIAAGQQVTADQLSQAQEFFAAVGRVVTVPESQLDAVTGLSGSGPGYVALIVE 181

Query: 186 AMADGAVLEGMDRKSALTLAAQTVLGAAKMALETGMHPGELKDMVTSPAGTTIQGIHSLE 245
           A++DG V  G+ R  A  LA QTV G A++  ETG HPGELKD V SP GTTI G+ +LE
Sbjct: 182 ALSDGGVAAGLPRAIATELAIQTVRGTAELLQETGWHPGELKDRVCSPGGTTIAGVATLE 241

Query: 246 EAGIRAAFMNAVIRASERSKELG 268
           + G+R+A + AV  A+ RS++LG
Sbjct: 242 QNGLRSALIEAVKAAALRSQQLG 264


Lambda     K      H
   0.313    0.130    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 193
Number of extensions: 10
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 270
Length of database: 264
Length adjustment: 25
Effective length of query: 245
Effective length of database: 239
Effective search space:    58555
Effective search space used:    58555
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate Synpcc7942_2058 Synpcc7942_2058 (pyrroline-5-carboxylate reductase)
to HMM TIGR00112 (proC: pyrroline-5-carboxylate reductase (EC 1.5.1.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00112.hmm
# target sequence database:        /tmp/gapView.22020.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00112  [M=263]
Accession:   TIGR00112
Description: proC: pyrroline-5-carboxylate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    1.3e-91  292.9   3.1    1.5e-91  292.7   3.1    1.0  1  lcl|FitnessBrowser__SynE:Synpcc7942_2058  Synpcc7942_2058 pyrroline-5-carb


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__SynE:Synpcc7942_2058  Synpcc7942_2058 pyrroline-5-carboxylate reductase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  292.7   3.1   1.5e-91   1.5e-91       1     263 []       5     263 ..       5     263 .. 0.98

  Alignments for each domain:
  == domain 1  score: 292.7 bits;  conditional E-value: 1.5e-91
                                 TIGR00112   1 iaiiGaGnmgeallsgllkkgakakkeilvierseeklaalakelgvevtsdaeeavkeadvvllavKP 69 
                                               ++iiG+G mgeall+ ll++g+ a++ + v e+ +++ + l +++gv+ + d++ea+ +ad+vlla+KP
  lcl|FitnessBrowser__SynE:Synpcc7942_2058   5 LGIIGGGVMGEALLARLLAQGQVAATAVTVSEPFAARCEFLNDRYGVRCVDDNAEAA-QADWVLLAIKP 72 
                                               68**************************************************99888.788******** PP

                                 TIGR00112  70 qdleevlaelkseektkeklliSilAGvtiekleqlleaekrvvRvmPNtaakvgagvtaiaassevse 138
                                               q++++++a l++   + ++l++SilAGv+++kle ++++ ++v+R+mPNt+a+vgagvtaiaa+++v++
  lcl|FitnessBrowser__SynE:Synpcc7942_2058  73 QVFDPAVAGLAD--LRSDRLILSILAGVPLAKLEAAFPH-EAVIRAMPNTPATVGAGVTAIAAGQQVTA 138
                                               ********9998..5579*******************97.78*************************** PP

                                 TIGR00112 139 eqkelveellkavGkvveveeklldavtalsGSgPAfvflliealadagvklGLpreeakelaaqtlkG 207
                                               +q ++++e ++avG vv+v+e++ldavt+lsGSgP +v+l++eal d+gv++GLpr+ a+ela qt+ G
  lcl|FitnessBrowser__SynE:Synpcc7942_2058 139 DQLSQAQEFFAAVGRVVTVPESQLDAVTGLSGSGPGYVALIVEALSDGGVAAGLPRAIATELAIQTVRG 207
                                               ********************************************************************* PP

                                 TIGR00112 208 aaklleesgehpalLkdkVtsPgGtTiaglavLeekgvrsavieaveaavkrseeL 263
                                               +a+ll+e+g hp +Lkd+V+sPgGtTiag+a+Le++g+rsa+ieav+aa+ rs++L
  lcl|FitnessBrowser__SynE:Synpcc7942_2058 208 TAELLQETGWHPGELKDRVCSPGGTTIAGVATLEQNGLRSALIEAVKAAALRSQQL 263
                                               ******************************************************98 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (263 nodes)
Target sequences:                          1  (264 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 6.25
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory