GapMind for Amino acid biosynthesis

 

Aligments for a candidate for cmutase in Synechococcus elongatus PCC 7942

Align Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 (characterized)
to candidate Synpcc7942_0881 Synpcc7942_0881 prephenate dehydratase

Query= SwissProt::P27603
         (365 letters)



>lcl|FitnessBrowser__SynE:Synpcc7942_0881 Synpcc7942_0881 prephenate
           dehydratase
          Length = 291

 Score =  140 bits (353), Expect = 4e-38
 Identities = 97/273 (35%), Positives = 147/273 (53%), Gaps = 12/273 (4%)

Query: 97  VAYLGPEGTFSQAAALKHFG-----HSVISKPMA--AIDEVFREVVAGAVNFGVVPVENS 149
           +A+LGP GT+++ AAL+            S+ +A  +I    + +  GAV++ VVPVENS
Sbjct: 6   IAHLGPVGTYAEMAALRFQAWLTQQDQQPSRLLACRSIPATLQTLADGAVDYAVVPVENS 65

Query: 150 TEGAVNHTLDSFLE-HDIVICGEVELRIHHHLLVGETTKTDRITRIYSHAQSLAQCRKWL 208
            EG+V  TLDS  +   + I   + L I H L+  E+ +T  I ++ SH Q+LAQC++WL
Sbjct: 66  VEGSVAATLDSLWQLPQLSIQRALILPIAHALISFESDRT-AIRQVLSHPQALAQCQQWL 124

Query: 209 DAHYPNVERVAVSSNADAAKRVKSEWNSAAIAGDMAAQLYGLSKLAEKIEDRPVNSTRFL 268
             H P  E +  +S  +A + ++     A IA   AA+LY +   +  I D P N TRF 
Sbjct: 125 QRHLPQAELIPANSTTEALQDLERHPQRAVIASTRAAELYQMPIQSFPINDSPDNRTRFW 184

Query: 269 IIGSQEVPPTGDDKTSIIVSM-RNKPGALHELLMPFHSNGIDLTRIETRPSRSGKWTYVF 327
           +I     P  G   TS+  S+  N PGAL + L       I+L+RIE+RP++     Y+F
Sbjct: 185 VISRSPTP--GGACTSLNFSLDANVPGALVKPLQILADRRINLSRIESRPTKRSLGEYLF 242

Query: 328 FIDCMGHHQDPLIKNVLEKIGHEAVALKVLGSY 360
           F+D     +DP I   ++ +      L+VLGSY
Sbjct: 243 FLDLEADLRDPAIAAAVQAVADCTEQLRVLGSY 275


Lambda     K      H
   0.319    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 235
Number of extensions: 13
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 365
Length of database: 291
Length adjustment: 28
Effective length of query: 337
Effective length of database: 263
Effective search space:    88631
Effective search space used:    88631
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory