Align argininosuccinate lyase (EC 4.3.2.1) (characterized)
to candidate GFF4252 PS417_21780 argininosuccinate lyase
Query= BRENDA::P9WPY7 (470 letters) >FitnessBrowser__WCS417:GFF4252 Length = 475 Score = 462 bits (1188), Expect = e-134 Identities = 239/457 (52%), Positives = 316/457 (69%), Gaps = 1/457 (0%) Query: 1 MSTNEGSLWGGRFAGGPSDALAALSKSTHFDWVLAPYDLTASRAHTMVLFRAGLLTEEQR 60 MS LWG RF GPS ALAALS+ + L PYDL SRAH L RAGLL E + Sbjct: 1 MSQPTDRLWGARFKTGPSAALAALSRCPERYFRLTPYDLAGSRAHARELQRAGLLDESET 60 Query: 61 DGLLAGLDSLAQDVADGSFGPLVTDEDVHAALERGLIDRVGPDLGGRLRAGRSRNDQVAA 120 L LDS+ D A G+ P + DEDVH +ER L +R+G LGG+LRAGRSRNDQ A Sbjct: 61 LRTLEALDSIGMDFAAGTLHPTLDDEDVHTFIERVLTERLGA-LGGKLRAGRSRNDQTAN 119 Query: 121 LFRMWLRDAVRRVATGVLDVVGALAEQAAAHPSAIMPGKTHLQSAQPILLAHHLLAHAHP 180 R++LRD R + T VL + AL +QA H ++ PG THLQ AQPI+ AHHLLAHA Sbjct: 120 DLRLFLRDHARTITTEVLGLQQALVDQAEQHIESVCPGFTHLQQAQPIVFAHHLLAHAQS 179 Query: 181 LLRDLDRIVDFDKRAAVSPYGSGALAGSSLGLDPDAIAADLGFSAAADNSVDATAARDFA 240 +LRD+ R+VD+D R A+SP G+ A+AGS++ P+ A ++G++ +NS+DA A+RD Sbjct: 180 MLRDVQRLVDWDARTALSPLGAAAMAGSAIARQPEHSAKEMGYTGPCENSIDAVASRDHV 239 Query: 241 AEAAFVFAMIAVDLSRLAEDIIVWSSTEFGYVTLHDSWSTGSSIMPQKKNPDIAELARGK 300 AE FV M+ V++SRL+E+ +WSS +F +V L D+++TGSSIMPQKKNPDIAELARGK Sbjct: 240 AEFLFVAGMLGVNISRLSEEFCLWSSRQFRWVVLDDAYATGSSIMPQKKNPDIAELARGK 299 Query: 301 SGRLIGNLAGLLATLKAQPLAYNRDLQEDKEPVFDSVAQLELLLPAMAGLVASLTFNVQR 360 +GRLIGNL GL++TLK+ PL+YNRDL EDK V DSV L L+LPAMAG+VA++ V+ Sbjct: 300 AGRLIGNLTGLMSTLKSLPLSYNRDLSEDKHSVLDSVDTLLLVLPAMAGMVATMKVQVEE 359 Query: 361 MAELAPAGYTLATDLAEWLVRQGVPFRSAHEAAGAAVRAAEQRGVGLQELTDDELAAISP 420 + AP G+TLAT++A+WL +GVPF+ AHE GA V+A E+ + L E + LA + Sbjct: 360 LRRQAPMGFTLATEVADWLATRGVPFKEAHEITGALVQACEKHEIELWEASSAMLAEVDA 419 Query: 421 ELTPQVREVLTIEGSVSARDCRGGTAPGRVAEQLNAI 457 L P+VR+ L++E +++AR GGTAP RV EQ+ + Sbjct: 420 RLLPEVRDCLSLEAAIAARSGWGGTAPERVREQIGRL 456 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 554 Number of extensions: 21 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 470 Length of database: 475 Length adjustment: 33 Effective length of query: 437 Effective length of database: 442 Effective search space: 193154 Effective search space used: 193154 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate GFF4252 PS417_21780 (argininosuccinate lyase)
to HMM TIGR00838 (argH: argininosuccinate lyase (EC 4.3.2.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00838.hmm # target sequence database: /tmp/gapView.19543.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00838 [M=455] Accession: TIGR00838 Description: argH: argininosuccinate lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-158 515.0 0.0 1.2e-158 514.8 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF4252 PS417_21780 argininosuccinate ly Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF4252 PS417_21780 argininosuccinate lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 514.8 0.0 1.2e-158 1.2e-158 1 453 [. 8 459 .. 8 461 .. 0.99 Alignments for each domain: == domain 1 score: 514.8 bits; conditional E-value: 1.2e-158 TIGR00838 1 lwggRlkkeldkavaefnaslsfDkelaeaDiegsiahtkaLakagilteeeakklieaLeelkeevkegklele 75 lwg R+k +++ a+a++++ + +l+ +D+ gs ah++ L++ag+l e e+ + eaL+++ + ++g+l+++ lcl|FitnessBrowser__WCS417:GFF4252 8 LWGARFKTGPSAALAALSRCPERYFRLTPYDLAGSRAHARELQRAGLLDESETLRTLEALDSIGMDFAAGTLHPT 82 7************************************************************************** PP TIGR00838 76 vdaeDiHlavErelidkvgedvgkklhtgrsRnDqvatdlrlylrdkvkelaealkdllkalvekAekevetlmp 150 d+eD+H+ +Er l++++g +g+kl++grsRnDq a dlrl+lrd+ ++++++++ l++alv++Ae+++e + p lcl|FitnessBrowser__WCS417:GFF4252 83 LDDEDVHTFIERVLTERLG-ALGGKLRAGRSRNDQTANDLRLFLRDHARTITTEVLGLQQALVDQAEQHIESVCP 156 *******************.******************************************************* PP TIGR00838 151 gytHLqrAqPitlaHhllayaemlerDleRlldalkRvnksPlGsgAlagtsfeidrellaelLgFdavvensld 225 g+tHLq+AqPi +aHhlla+a+++ rD++Rl+d R++ sPlG++A+ag++ + ++e +a+ +g+ +ens+d lcl|FitnessBrowser__WCS417:GFF4252 157 GFTHLQQAQPIVFAHHLLAHAQSMLRDVQRLVDWDARTALSPLGAAAMAGSAIARQPEHSAKEMGYTGPCENSID 231 *************************************************************************** PP TIGR00838 226 avsdRDfiiEllsaaallmvhlsrlaEelilfsseEfgfvelsdevssgssimPqKKnpDvaEliRgktgrviGn 300 av++RD+++E+l++a +l v++srl+Ee+ l+ss+ f +v l+d++++gssimPqKKnpD+aEl+Rgk+gr+iGn lcl|FitnessBrowser__WCS417:GFF4252 232 AVASRDHVAEFLFVAGMLGVNISRLSEEFCLWSSRQFRWVVLDDAYATGSSIMPQKKNPDIAELARGKAGRLIGN 306 *************************************************************************** PP TIGR00838 301 ltglltilKalPlaYnkDlqEdkealfdalktveellevvtgllkelkvnkerleeaakknfalatdlAdylvrk 375 ltgl+ +lK+lPl+Yn+Dl Edk ++ d+++t+ +l +++g+++++kv+ e+l+++a +f+lat++Ad+l+++ lcl|FitnessBrowser__WCS417:GFF4252 307 LTGLMSTLKSLPLSYNRDLSEDKHSVLDSVDTLLLVLPAMAGMVATMKVQVEELRRQAPMGFTLATEVADWLATR 381 *************************************************************************** PP TIGR00838 376 GvPFReaheivGevvakaiekGkkleeltleelqklsekleedvlevldleeavekrdakGGtakeevekaieea 450 GvPF+eahei+G++v++++++ ++l e + + l++++ +l ++v++ l+le a+++r+ GGta+e+v+++i + lcl|FitnessBrowser__WCS417:GFF4252 382 GVPFKEAHEITGALVQACEKHEIELWEASSAMLAEVDARLLPEVRDCLSLEAAIAARSGWGGTAPERVREQIGRL 456 ************************************************************************998 PP TIGR00838 451 kae 453 k lcl|FitnessBrowser__WCS417:GFF4252 457 KIA 459 865 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (455 nodes) Target sequences: 1 (475 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 7.72 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory