Align Homoserine O-acetyltransferase; HAT; Homoserine transacetylase; HTA; EC 2.3.1.31 (characterized)
to candidate GFF5222 PS417_26740 homoserine acetyltransferase
Query= SwissProt::F7XKY8 (492 letters) >FitnessBrowser__WCS417:GFF5222 Length = 379 Score = 372 bits (955), Expect = e-107 Identities = 188/372 (50%), Positives = 257/372 (69%), Gaps = 6/372 (1%) Query: 5 SLGIVETKYYHLEEELKLESGRKISDVTIAYEAYGTLNRDKNNVILVCHALTGDAHAAGW 64 S+G+V + H E L L GR + + YE YG LN +N +L+CHAL+G HAAG+ Sbjct: 9 SVGLVVPQVAHFSEPLALACGRALPAYDLIYETYGQLNATASNAVLICHALSGHHHAAGF 68 Query: 65 HKGDN-KPGWWDILIGPGKCLDTDKYFIVCSNVIGGCRGSTGPSSIDPETGKPYGLSFPV 123 H D KPGWWD IGPGK +DT+K+F+V N +GGC GSTGPSSI+PETGKP+G FPV Sbjct: 69 HSVDERKPGWWDSCIGPGKPIDTNKFFVVSLNNLGGCNGSTGPSSINPETGKPFGADFPV 128 Query: 124 ITIKDMVNAQKKLLDHLGISSIYAVIGGSMGGLQVLQWSVSYPDYINKAIALAASAYSSP 183 +T++D V++Q +L D LGI+ AVIGGS+GG+Q LQW+++YPD + +A+A++ S Sbjct: 129 LTVEDWVHSQARLADLLGINQWAAVIGGSLGGMQALQWTITYPDRVRHCLAIASAPKLSA 188 Query: 184 QQIAFNEVARIAIISDPEWNKGNYYYSRQ-PSHGLALARMIGHITYLSDESMREKFGREL 242 Q IAFNEVAR AI++DPE++ G++ + P GL LARM+GHITYLSD+SM EKFGR L Sbjct: 189 QNIAFNEVARQAILTDPEFHGGSFQEAGVIPKRGLMLARMVGHITYLSDDSMGEKFGRGL 248 Query: 243 QDRDRYNYDL-SMDFQVESYLHYKGRSFTERFDANSYLYITKAVDYFDLTEN--GSLIDG 299 + ++ NYD S++FQVESYL Y+G F+ RFDAN+YL +TKA+DYFD N L Sbjct: 249 KS-EKLNYDFHSVEFQVESYLRYQGEEFSGRFDANTYLLMTKALDYFDPAANFDDDLAKT 307 Query: 300 LKDIKAKCLIIAVTSDWLYPPYQSKDIVMALNANNVDVTYREIESNYGHDAFLLEAGQLN 359 + AK +++ T+DW + P +S+++V AL A DV Y EI++ GHDAFL+ + Sbjct: 308 FEGATAKFCVMSFTTDWRFSPARSRELVDALMAARKDVCYLEIDAPQGHDAFLIPIPRYL 367 Query: 360 YVIGGFLNNITV 371 G ++N ITV Sbjct: 368 QAFGNYMNRITV 379 Lambda K H 0.318 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 510 Number of extensions: 24 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 492 Length of database: 379 Length adjustment: 32 Effective length of query: 460 Effective length of database: 347 Effective search space: 159620 Effective search space used: 159620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate GFF5222 PS417_26740 (homoserine acetyltransferase)
to HMM TIGR01392 (metX: homoserine O-acetyltransferase (EC 2.3.1.31))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01392.hmm # target sequence database: /tmp/gapView.13902.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01392 [M=351] Accession: TIGR01392 Description: homoserO_Ac_trn: homoserine O-acetyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.3e-141 455.2 0.0 8.4e-141 455.0 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF5222 PS417_26740 homoserine acetyltra Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF5222 PS417_26740 homoserine acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 455.0 0.0 8.4e-141 8.4e-141 3 345 .. 22 369 .. 20 375 .. 0.97 Alignments for each domain: == domain 1 score: 455.0 bits; conditional E-value: 8.4e-141 TIGR01392 3 eeltlesGevlsevevayktyGtlnaerdNavlvcHaltgsahvagkadeedk..GWWdellGpgraldtsryfv 75 e+l l +G l+ +++ y+tyG+lna +Navl+cHal+g++h+ag ++ +++ GWWd+ +Gpg+++dt+++fv lcl|FitnessBrowser__WCS417:GFF5222 22 EPLALACGRALPAYDLIYETYGQLNATASNAVLICHALSGHHHAAGFHSVDERkpGWWDSCIGPGKPIDTNKFFV 96 789*******************************************998777678******************** PP TIGR01392 76 vclNvlGsckGstgPlsinpetgkpygaefPlvtirDlvkaqkalldsLgveklaavvGgSlGGmqalewalsyp 150 v+lN+lG+c+GstgP+sinpetgkp+ga+fP++t++D+v+ q++l+d Lg++++aav+GgSlGGmqal+w+++yp lcl|FitnessBrowser__WCS417:GFF5222 97 VSLNNLGGCNGSTGPSSINPETGKPFGADFPVLTVEDWVHSQARLADLLGINQWAAVIGGSLGGMQALQWTITYP 171 *************************************************************************** PP TIGR01392 151 ervkkivvlatsarasaqaiafnevqrqailsDpeyndGeyaeee.qPekGLalARmlalltYrseesleerfgr 224 +rv++++++a+++++saq+iafnev+rqail+Dpe+++G+++e + P++GL lARm++++tY+s++s+ e+fgr lcl|FitnessBrowser__WCS417:GFF5222 172 DRVRHCLAIASAPKLSAQNIAFNEVARQAILTDPEFHGGSFQEAGvIPKRGLMLARMVGHITYLSDDSMGEKFGR 246 *********************************************9***************************** PP TIGR01392 225 eakseeslassleeefsvesylryqgkkfverFdAnsYllltkaldthdlargrrdslkealkkikapvlvvgie 299 kse+ ++ ef+vesylryqg++f rFdAn+Yll+tkald++d a++ +d+l+++++ ++a+++v++++ lcl|FitnessBrowser__WCS417:GFF5222 247 GLKSEKLNYDFHSVEFQVESYLRYQGEEFSGRFDANTYLLMTKALDYFDPAANFDDDLAKTFEGATAKFCVMSFT 321 ***9977777779************************************************************** PP TIGR01392 300 sDllftleeqeelakalkaakle..yaeieseeGHDaFllekekveel 345 +D++f++++++el++al aa+++ y ei+ ++GHDaFl+ ++ ++ lcl|FitnessBrowser__WCS417:GFF5222 322 TDWRFSPARSRELVDALMAARKDvcYLEIDAPQGHDAFLIPIPRYLQA 369 *******************999999**************988776665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (351 nodes) Target sequences: 1 (379 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 10.64 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory