Align Phosphoserine phosphatase; PSP; EC 3.1.3.3 (characterized)
to candidate GFF5391 PS417_27600 HAD family hydrolase
Query= SwissProt::Q72H00 (249 letters) >FitnessBrowser__WCS417:GFF5391 Length = 231 Score = 101 bits (252), Expect = 1e-26 Identities = 88/254 (34%), Positives = 118/254 (46%), Gaps = 44/254 (17%) Query: 1 MKLLLLDLDDTLLQDLPVSRAVLEDLGRKAGVEGFFARVKARAEALFREAPFYPW----A 56 +KL+ DLDDTL ++PV + AEA RE W A Sbjct: 3 IKLITFDLDDTLWDNVPVIIS---------------------AEASMRE-----WLTVNA 36 Query: 57 EAIGHSALEALWARYSTPGLEA---LAAWAGPFRERVFREALEEAGGAPERARELA---- 109 +G LE +A LE L R RV A EEAG A E+A Sbjct: 37 AKVGDLPLEH-FASLRQQVLERHPELKHRISLLRHRVLMHAFEEAGYPQPEATEMADVCF 95 Query: 110 EAFFRERRRYPLYPEAEAFLAEARRRGLALALLTNGVPDLQREKLVGAGLAHHFSLVLIS 169 EAF R + +PEAE L +A R+ L ++TNG D+QR GLA +F L + Sbjct: 96 EAFIHARHQLAPFPEAEPML-KALRQHFLLGVITNGNADVQR-----VGLADYFHFALRA 149 Query: 170 GEVGIGKPDPRLFRMALCAFGVAPEEAAMVGDNPQKDVRGARLAGVRAVWVDRGLRPEDP 229 ++GI KPD RLF+ AL GV A +GD+P D+ GA+ AG+RAVW + + + Sbjct: 150 EDIGIAKPDARLFQEALQRGGVDASAAVHIGDHPGDDIAGAQQAGLRAVWFNPTGKVWEA 209 Query: 230 EASPDLRVGDLREV 243 + PD V L E+ Sbjct: 210 DKRPDAEVRSLAEL 223 Lambda K H 0.322 0.140 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 159 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 249 Length of database: 231 Length adjustment: 23 Effective length of query: 226 Effective length of database: 208 Effective search space: 47008 Effective search space used: 47008 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory