GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapX in Acidovorax sp. GW101-3H11

Align Probable N-acetyl-LL-diaminopimelate aminotransferase; Putative aminotransferase A; EC 2.6.1.- (characterized)
to candidate Ac3H11_1602 Aspartate aminotransferase (EC 2.6.1.1)

Query= SwissProt::P16524
         (393 letters)



>lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_1602 Aspartate
           aminotransferase (EC 2.6.1.1)
          Length = 408

 Score =  201 bits (511), Expect = 3e-56
 Identities = 131/373 (35%), Positives = 202/373 (54%), Gaps = 20/373 (5%)

Query: 21  SNLVAQHEDVISLTIGQPDFFTPHHVKAAAKKAIDENVTSYTPNAGYLELRQAVQLYMKK 80
           + L  Q + VI L +G+PDF TP H+  AA++A+    T YT   G  EL+ A+Q   K 
Sbjct: 32  NQLKRQGQPVIVLGLGEPDFDTPAHILEAAQQAMARGETHYTVLDGTAELKAAIQHKFKH 91

Query: 81  KADFNYDAESEIIITTGASQAIDAAFRTILSPGDEVIMPGPIYPGYEPIINLCGAKPVIV 140
               ++   +EI    GA Q +  A    ++PGDEVI+P P +  Y  ++ + G  PV+V
Sbjct: 92  YNGLDFQL-NEITAGAGAKQILYNALMASVNPGDEVILPAPYWTSYADMVLIAGGVPVVV 150

Query: 141 DTT-SHGFKLTARLIEDALTPNTKCVVLPYPSNPTGVTLSEEELKSIAALLKGR-NVFVL 198
             T ++GF++T   +E A+TP T+ V +  PSNP+G   S E+L+ +  +++    V++L
Sbjct: 151 PCTEANGFRITPEQLEAAITPRTRWVFINSPSNPSGAAYSAEQLRPVLEVVERHPQVWLL 210

Query: 199 SDEIYSELTYDRPHYS----IATYLRDQTIVINGLSKSHSMTGWRIGFLFAPKDIAKHIL 254
           +D+IY  + YD   ++    +   LRD+T+ +NG+SK+++MTGWR+G+   PK +   + 
Sbjct: 211 ADDIYEHILYDGRAFATPAAVLPSLRDRTLTVNGVSKAYAMTGWRLGYGAGPKALIAAMA 270

Query: 255 KVHQYNVSCASSISQKAALEAVTNGFDDALIMREQYKKRLDYVYDRL-VSMGLDVVKPSG 313
            V     SC SSISQ AA+ A+T   D      + ++ R D V   L VS GL    P G
Sbjct: 271 VVQSQATSCPSSISQAAAVAALTGPQDVVRERCQAFQDRRDLVVAALNVSPGLRCRVPEG 330

Query: 314 AFYIFPSIK-SFGMTS---------FDFSMALLEDAGVALVPGSSFSTYGEGYVRLSFAC 363
           AFY F S + + G T+          DF   LL +  VA+VPG         Y R+S+A 
Sbjct: 331 AFYTFASCEGALGRTTPGGLLLRTDADFCAYLLREHHVAVVPGGVLGL--APYFRISYAA 388

Query: 364 SMDTLREGLDRLE 376
           S   L+E   R++
Sbjct: 389 STADLQEACARIQ 401


Lambda     K      H
   0.319    0.135    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 353
Number of extensions: 16
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 393
Length of database: 408
Length adjustment: 31
Effective length of query: 362
Effective length of database: 377
Effective search space:   136474
Effective search space used:   136474
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory