Alignments for a candidate for cmutase in Acidovorax sp. GW101-3H11

GapMind for Amino acid biosynthesis

Alignments for a candidate for cmutase in Acidovorax sp. GW101-3H11

Align Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 (characterized)
to candidate Ac3H11_2574 Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51)

Query= SwissProt::P27603
         (365 letters)



>FitnessBrowser__acidovorax_3H11:Ac3H11_2574
          Length = 351

 Score =  290 bits (743), Expect = 3e-83
 Identities = 157/354 (44%), Positives = 220/354 (62%), Gaps = 10/354 (2%)

Query: 12  VRIDSLDERILDLISERARCAQEVARVKTASWPKAEEAVFYRPEREAWVLKHIMELNKGP 71
           V+IDS+D+++L L+++RA  A++V  VK     K E   F+RP+R A V+  I   N GP
Sbjct: 1   VQIDSIDQQLLTLLNQRALVAEQVGEVK-----KREGTPFFRPDRVAQVIDKITHANPGP 55

Query: 72  LDNEEMARLFREIMSSCLALEQPLRVAYLGPEGTFSQAAALKHFGHSVISKPMAAIDEVF 131
           L    +A ++REIMS+CLALE P RVA LGPEGTF + AA++ FG +      A  DEVF
Sbjct: 56  LKGPHVAAIWREIMSACLALESPQRVAVLGPEGTFCEQAAIEFFGGAADLMYCANFDEVF 115

Query: 132 REVVAGAVNFGVVPVENSTEGAVNHTLDSFLEHDIVICGEVELRIHHHLLVGETTKTDRI 191
               AG+  +GVV VENSTEG V  +LD FL     + GEV L + H+LL   T   D I
Sbjct: 116 HATAAGSAQYGVVGVENSTEGVVTRSLDLFLHTPTHVVGEVSLLVRHNLL-RTTNSLDGI 174

Query: 192 TRIYSHAQSLAQCRKWLDAHYPNVERVAVSSNADAAKRVKSEWNSAAIAGDMAAQLYGLS 251
             + +H Q+LAQC+ WL  H P+ ER  VSSNA+ A+   +    AA++ + AA  +GL 
Sbjct: 175 EAVLAHPQALAQCQAWLSKHLPHAERRPVSSNAEGARLATTNPAWAALSSERAATQFGLH 234

Query: 252 KLAEKIEDRPVNSTRFLII----GSQEVPPTGDDKTSIIVSMRNKPGALHELLMPFHSNG 307
            ++  I+D   N TRF II      Q  PP+G D TS++VS+ N+PGA+H+LL+P  ++G
Sbjct: 235 IVSHAIQDDAYNRTRFAIICLPHTLQTPPPSGKDCTSLVVSVPNQPGAVHDLLVPLKTHG 294

Query: 308 IDLTRIETRPSRSGKWTYVFFIDCMGHHQDPLIKNVLEKIGHEAVALKVLGSYP 361
           + +TR E+RP+R+G+W Y F+ID  GH   P +   LE++       KVLG+YP
Sbjct: 295 VSMTRFESRPARTGQWEYYFYIDLDGHPAQPHVARALEELRSLCAFYKVLGTYP 348


Lambda     K      H
   0.319    0.133    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 350
Number of extensions: 12
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 365
Length of database: 351
Length adjustment: 29
Effective length of query: 336
Effective length of database: 322
Effective search space:   108192
Effective search space used:   108192
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate Ac3H11_2574 (Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51))
to HMM TIGR01807 (pheA: chorismate mutase (EC 5.4.99.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01807.hmm
# target sequence database:        /tmp/gapView.27871.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01807  [M=76]
Accession:   TIGR01807
Description: CM_P2: chorismate mutase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                        -----------
    8.7e-28   82.7   0.0    1.5e-27   82.0   0.0    1.4  1  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2574  Chorismate mutase I (EC 5.4.99.5


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2574  Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !   82.0   0.0   1.5e-27   1.5e-27       7      76 .]       2      69 ..       1      69 [. 0.99

  Alignments for each domain:
  == domain 1  score: 82.0 bits;  conditional E-value: 1.5e-27
                                        TIGR01807  7 kiDaiDdrildLlseRaklakavgelKkksaseaviYRPeREaavlrrlkelnkGpLdqeavar 70
                                                     +iD+iD+++l+Ll++Ra +a++vge+Kk+  ++++++RP+R a+v+ ++ ++n+GpL+   va+
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2574  2 QIDSIDQQLLTLLNQRALVAEQVGEVKKR--EGTPFFRPDRVAQVIDKITHANPGPLKGPHVAA 63
                                                     8****************************..********************************* PP

                                        TIGR01807 71 ifrEim 76
                                                     i+rEim
  lcl|FitnessBrowser__acidovorax_3H11:Ac3H11_2574 64 IWREIM 69
                                                     *****9 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (76 nodes)
Target sequences:                          1  (351 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 9.52
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis