Align Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized)
to candidate Ac3H11_1086 Glyoxylate carboligase (EC 4.1.1.47)
Query= curated2:O08353 (599 letters) >FitnessBrowser__acidovorax_3H11:Ac3H11_1086 Length = 596 Score = 321 bits (822), Expect = 6e-92 Identities = 188/592 (31%), Positives = 318/592 (53%), Gaps = 39/592 (6%) Query: 1 MNGAEAMIKALEAEKVEILFGYPGGALLPFYDALH-HSDLIHLLTRHEQAAAHAADGYAR 59 M EA +K +E E V + FG PG A+ P Y AL H + H+L RH + A+H A+GY R Sbjct: 4 MKAIEAAVKVMEKEGVSVAFGVPGAAINPLYAALKAHGGIGHILARHVEGASHMAEGYTR 63 Query: 60 A-SGKVGVCIGTSGPGATNLVTGVATAHSDSSPMVALTGQVPTKLIGNDAFQEIDALGLF 118 A +G +GVCIGTSGP T+++TG+ +A +DS P++ +TGQ P + + FQ +D + Sbjct: 64 AVAGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITGQAPRARLHKEDFQAVDIASIA 123 Query: 119 MPIVKHNFQIQKTCQIPEIFRSAFEIAQTGRPGPVHIDLPKDVQELELDIDKHPIPSKVK 178 + K + + Q+P F+ AF + ++GRPGPV IDLP DVQ E++ D + Sbjct: 124 KSVTKWATTVLEPAQVPGAFQQAFHLMRSGRPGPVLIDLPIDVQLAEIEFDP---DTYEP 180 Query: 179 LIGYNPTTIGHPRQIKKAIKLIASAKRPIILAGGGVLLSGANEELLKLVELLNIPVCTTL 238 L+ Y P QI+KA+ ++ +A+RP+I+AGGG++ + A++ L++ E+L +PV TL Sbjct: 181 LVPYKPAAT--RAQIEKALSMLNAAERPLIVAGGGIINADASDLLVQFAEVLGVPVIPTL 238 Query: 239 MGKGCISENHPLALGMVGMH-GTKPANYCLSESDVLISIGCRFSDRITGDIKSFATNAKI 297 MG G I ++HPL +GM G+ + N L SD ++ IG R+++R TG + + K Sbjct: 239 MGWGSIPDDHPLMVGMCGLQTSQRYGNATLLASDFVLGIGNRWANRHTGSVDVYTKGRKF 298 Query: 298 IHIDIDPAEIGKNVNVDVPIVGDAKLILKEVIKQLDYIINKDSKENNDKENISQWIENVN 357 +H+DI+P +IG+ D IV DA LK ++ + ++ K + S W Sbjct: 299 VHVDIEPTQIGRVFAPDFGIVSDAGAALKLFVE-----VAREWKAVGQLRDRSTWAAECQ 353 Query: 358 SLKKS--SIPVMDYDDIPIKPQKIVKELMAVIDDLNINKNTIITTDVGQNQMWMAHYFKT 415 K S + +D +P+KPQ++ + + + + K+ + +G +Q+ A + Sbjct: 354 GRKHSVDYLRKTHFDSVPMKPQRVYQCM-----NRALGKDVCYVSTIGLSQIAGAQFLHV 408 Query: 416 QTPRSFLSSGGLGTMGFGFPSAIGAKVAKPDSKVICITGDGGFMMNCQELGTIAEYNIPV 475 PR +++ G G +G+ P+A+G +VA P K++ ++GD F +EL A++ +P Sbjct: 409 YQPRHWINCGQAGPLGWTVPAALGVRVADPARKIVALSGDYDFQFMIEELAVGAQFKLPY 468 Query: 476 VICIFDNRTLGMVYQWQNLF------------YGKRQCSVNFGGAPDFIKLAESYGIKAR 523 + + +N LG++ Q Q F + V G D +K+ E G KA Sbjct: 469 LHVLVNNSYLGLIRQAQRTFSIDYCVQLAFDNVNMAEGEVARGYGVDHVKVVEGLGCKAI 528 Query: 524 RIESPNEINEALKEA----INCDEPYLLDFAIDPSSALSMVPPGAKLTNIID 571 R+ P E AL +A P +++ ++ + ++M G ++ NI++ Sbjct: 529 RVHRPEEFAPALAQAEAWMAEHRTPVVIEVILERVTNIAM---GTEIDNIVE 577 Lambda K H 0.319 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 801 Number of extensions: 43 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 599 Length of database: 596 Length adjustment: 37 Effective length of query: 562 Effective length of database: 559 Effective search space: 314158 Effective search space used: 314158 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory